Dmel\P{EPgy2}Arfip^EY11874 Insertion

General Information
Symbol	Dmel\P{EPgy2}Arfip^EY11874	Species	D. melanogaster
Name		FlyBase ID	FBti0057674
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EPgy2}	Expression data
Affected gene(s)	Arfip	Viability / fertility	fertile, viable
Causes allele(s)	Arfip^EY11874	Stock availability	1 publicly available
LINE ID	EY11874
Genomic Location
Chromosomal location	3R ( 86D8 )	Sequence location	3R:7,060,890..7,060,890 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EPgy2}.EY A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w[+mC] and the mini-yellow marker y[+mDint2], and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Bellen et al., 2004, Bellen et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3R:7,060,890..7,060,890 [-] (Gene Disruption Project members, 2001-)
Orientation
Cytological location (computed by FlyBase)	86D8 ( inferred by FlyBase from sequence location )
Cytological location (reported)	86D10 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CL868525 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	P{EPgy2}
Location-dependent role	mobile activating element (UASG) (Bellen et al., 2004)
Size	10.908Kb
Associated alleles	Scer\UAS^14x.P\T TrlBR^cRa w^+mC y^+mDint2
Molecular map
Affected Gene(s)
Insertion may affect gene	Arfip (Gene Disruption Project members, 2001-, Korolchuk et al., 2007, Cheli et al., 2010)
Alleles and Phenotypes
Causes alleles	Arfip^EY11874 (Gene Disruption Project members, 2001-, Korolchuk et al., 2007, Cheli et al., 2010)

Lethality References viable (Gene Disruption Project members, 2001-)
Sterility References fertile (Gene Disruption Project members, 2001-)
Phenotype Manifest In

Detailed Description
Statement Reference Expression of CG17184[EY11874] under the control of Scer\GAL4[GMR.PF] has no effect on the reduced red eye pigment phenotype of blos1[ex2] flies. (Cheli et al., 2010) Primary cultured haemocytes from homozygous larvae internalise maleylated bovine serum albumin (mBSA) normally. (Korolchuk et al., 2007)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EY11874 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	20706 y¹ w^67c23; P{EPgy2}Arfip^EY11874
Linkouts
Comments
	Location 3R:7060890-7060891 confirmed by FlyBase alignment of dbGSS accession CL868525 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{EPgy2}Arfip^EY11874 P{EPgy2}CG17184^EY11874 (Cheli et al., 2010, FlyBase, 1992-, FlyBase, 2005) P{EPgy2}EY11874 (FlyBase, 1992-) P{Epgy2}EY11874 (Korolchuk et al., 2007)
Secondary FlyBase IDs

References ( 8 )
Research paper	Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Cheli et al., 2010, Hum. Mol. Genet. 19(5): 861--878 Genetic modifiers of abnormal organelle biogenesis in a Drosophila model of BLOC-1 deficiency. [FBrf0209903] Korolchuk et al., 2007, J. Cell Sci. 120(24): 4367--4376 Drosophila Vps35 function is necessary for normal endocytic trafficking and actin cytoskeleton organisation. [FBrf0202367] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]
FlyBase analysis	FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EPgy2}ArfipEY11874 Insertion

Dmel\P{EPgy2}Arfip^EY11874 Insertion