- Tools
- Downloads
- Links
- Community
- About
- Help
FB2026_01
,
released March 12, 2026
[39D3-39D3];[39E2-39E2];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
39D3;39E2
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Fails to complement Df(2L)Exel7081. Inferred to overlap with: Df(2L)Exel7081.
Homozygous clones are not recovered in the wing disc.
Four copies of M{3xHisGU.H3.2-H3.3} rescue the formation of homozygous Df(2L)BSC104 clones in the wing disc. Homozygous Df(2L)BSC104 animals carrying four copies of M{3xHisGU.H3.2-H3.3} survive into the first instar larval stage with no obvious patterning defects.
Four copies of M{3xHisGU.ΔH3.2} rescue the formation of homozygous Df(2L)BSC104 clones in the wing disc, although they are severely growth impaired compared to their wild-type twin spots.
Two copies of M{3xHisGU.ΔH3.2} do not rescue the formation of homozygous Df(2L)BSC104 clones in the wing disc in a His3.3AKO His3.3BKO background.
Two or four copies of M{3xHisGU.wt} rescue the formation of homozygous Df(2L)BSC104 clones in the wing disc.
Clones triply homozygous for His3.3AKO, His3.3BKO and Df(2L)BSC104 survive if they are carrying both His3.3BK4R and two copies of M{3xHisGU.H3.2.K4R}, although the clones are smaller than their wild-type twin spots.
Clones triply homozygous for His3.3AKO, His3.3BKO and Df(2L)BSC104 survive if they are carrying both His3.3BK4A and two copies of M{3xHisGU.H3.2.K4A}, although the clones are smaller than their wild-type twin spots.
Presence of P+PBac{XP5.WH5}BSC104 was verified using the PCR methods and primers described in FBrf0175003.
The cytological breakpoints of Df(2L)BSC104 predicted from the PBac{WH}f05491 and P{XP}d03256 Release 3 genome coordinates are 39D5;39E2.