[51C2-51C2];[51D1-51D1];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
51C2;51D1
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Df(2R)BSC429 is homozygous lethal.
The presence of P+PBac{XP5.RB3}BSC429 was verified using the PCR methods and primers described in FBrf0175003, with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The cytological breakpoints of Df(2R)BSC429 predicted from the Release 5 genomic coordinates of the progenitor PBac{RB}CR30478e00165 and P{XP}d03333 insertion sites are 51C2;51D1.