FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\βTub85D6
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General Information
Symbol
Dmel\βTub85D6
Species
D. melanogaster
Name
FlyBase ID
FBal0000027
Feature type
allele
Associated gene
Dmel\βTub85D
Associated Insertion(s)
Carried in Construct
Also Known As
B2t6, β2t6
Key Links
Genomic Maps

Allele class

loss of function allele

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Jattani et al., 2009)

loss of function allele

(Hutchens et al., 1997)
Mutagen
ethyl methanesulfonate
(Fackenthal et al., 1995)
Progenitor genotype
Cytology
Description

Amino acid substitution within an internal cluster of variable amino acids that has been identified as an isotype-defining domain in vertebrate β-tubulins. Amino acid replacement: G56D. Nucleotide substitution: G?A.

(Fackenthal et al., 1995)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:9,409,886..9,409,886 [-]
Nucleotide change:

G9409886A

Reported nucleotide change:

G?A

Amino acid change:

G56D | betaTub85D-PA

Reported amino acid change:

G56D

(Fackenthal et al., 1995)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In embryos homozygous for βTub85D6, the distal tip of the salivary gland turns as in wild-type by stage 12. By stage 14, however, only the distal half of the mutant salivary glands are turned posteriorly.

      Homozygous βTub85D6 mutant embryos display defects in fusion competent myoblast migration at stage 14.

      Trans-heterozygous stage 13 βTub85Dn/βTub85D6 embryos display salivary gland migration defects.

      Stage 13 βTub85D6/Df(3R)by10 embryos display salivary gland migration defects.

      (Jattani et al., 2009)

      Misspecification of the morphology of the doublet of the sperm tail axoneme.

      (Fackenthal et al., 1995)

      Affects microtubule function during spermatogenesis.

      (Green et al., 1990)

      βTub85D6/βTub85Dn males are sterile. Chromosome movement during meiosis appears normal in homozygous males, and each nucleus is usually paired with a single, normal-sized mitochondrial derivative in the developing spermatids. Nuclear shaping occurs normally and nuclear alignment occurs relatively normally during spermatogenesis. Mature axonemes appear abnormal, with a core of densely staining material present in the A subfibres of the outer doublet microtubules, in addition to the core of densely staining material that is normally present in the central pair and accessory tubules. Occasionally, filled outer doublet B tubules are also seen.

      (Fuller et al., 1988)

      Young axonemes in the spermatids of homozygous males look morphologically normal. Mature axonemes have a subtle defect - the outer doublet microtubules fill in with the densely staining material normally found only in the central pair and accessory tubules. Nuclear shaping is normal.

      (Fuller, 1986)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Statement
      Reference

      βTub85D6 is a non-suppressor of male sterile phenotype of βTub60D::βTub85Dstar

      (Fackenthal et al., 1995)
      Other
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      βTub85D6 heterozygotes fail to rescue the male sterile phenotype of βTub60D::βTub85Dstar; no sperm is present in the seminal vesicles. βTub60D::βTub85Dstar and βTub85D6 isoforms disrupt a common aspect of axoneme assembly.

      (Fackenthal et al., 1995)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Acidic electrophoretic variant. The protein encoded by βTub85D7 is stable and capable of forming an αβ tubulin heterodimer.

      (Fuller et al., 1988)

      Class II allele. P{β2β3*-pW8} and βTub85D6 isoforms disrupt a common aspect of axoneme assembly.

      (Fackenthal et al., 1995)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      B2t6
      (Hutchens et al., 1997, Fackenthal et al., 1995, Hoyle et al., 1995, Fuller, 1993, Fuller et al., 1988)
      Tβ26
      β2t6
      (Jattani et al., 2009, Fuller, 1986)
      βTub85D6
      Name Synonyms
      Secondary FlyBase IDs
        References (8)