FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\vnddd-4
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General Information
Symbol
Dmel\vnddd-4
Species
D. melanogaster
Name
FlyBase ID
FBal0002307
Feature type
allele
Associated gene
Dmel\vn
Associated Insertion(s)
Carried in Construct
Also Known As
vndddRY, vnRY, veinRY
Key Links
Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Schnepp et al., 1996)
Mutagen
ethyl methanesulfonate
(Simcox et al., 1996, Wurst et al., 1984)
Progenitor genotype
Cytology
Description

Disrupts the vn transcribed region, breaking in the major intron, intron 1.

(Schnepp et al., 1996)

Deletion that removes part of the vn region and extends proximally.

(Simcox et al., 1996)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      vnddd-4/vnL6 third instar larvae exhibit strongly reduced axon wrapping index (proportion of axon clusters wrapped by wrapping glia) relative to controls. In wild-type larvae, subperineurial glial cells form autocellular contacts with pronounced septate junction, the length of these autocellular contacts is significantly reduced in vnddd-4/vnL6 larvae.

      (Matzat et al., 2015)

      Early CNS development is essentially normal in mutant embryos.

      (Skeath, 1998)

      vnddd-3/vnddd-4 embryos exhibit a wild type phenotype. Leg and eye/antenna vnddd-3/vnddd-4 mutant discs can be in vivo cultured. Wing and haltere discs cannot.

      (Simcox, 1997)

      Embryos are shorter and the Keilin's organs and ventral black dots are closer together than wild type. Ventral cuticle is deleted.

      (Schnepp et al., 1996)

      Individuals transheterozygous with a strong EMS vn mutation exhibit small wings. Transheterozygotes with vnL6 are pupal/pharate adult lethal.

      (Simcox et al., 1996)

      vnddd-4/vnddd-5 transheterozygotes show cold sensitive larval lethality - embryos allowed to develop at 17oC die as larvae, but are not missing wing or haltere discs.

      (Wurst et al., 1984)

      embryonic lethal

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Enhancer of
      Statement
      Reference

      vnddd-4 is an enhancer of phenotype of spi1

      (Schnepp et al., 1996)
      NOT Suppressor of
      Statement
      Reference

      vnddd-4 is a non-suppressor of phenotype of Src42ASu(Raf)1-1

      (Zhang et al., 1999)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Clones expressing rhoScer\UAS.cGa under the control of Scer\GAL4αTub84B.PZ induced in the first larval instar can stimulate proliferation in vnL6/vnddd-4 wing discs, irrespective of the position of the clone within the wing disc. Clones expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4αTub84B.PC usually do not stimulate the growth of vnL6/vnddd-4 wing discs. A few discs do show a modest increase in size, but this rescue appears to be due to a cell-autonomous rescue of proliferation within the Ras85DV12.Scer\UAS expressing clone. Clones expressing apScer\UAS.cOa under the control of Scer\GAL4αTub84B.PZ induced in the first larval instar can rescue growth and differentiation vnL6/vnddd-4 wing discs.

      (Zecca and Struhl, 2002)

      Does not suppress the ability of Src42ASu(phl)1-1 to suppress the lethality of phl1/Y flies.

      (Zhang et al., 1999)

      rho7M43, vnddd-4 double mutant embryos show embryonic CNS neuroblast phenotypes that are indistinguishable from those cause by Egfr mutants. Only two neuroblast columns form, RP2 motorneuron almost never forms, half of the MP2s are missing. The 5-2 and MP2 phenotypes are slightly stronger than for Egfrf2.

      (Skeath, 1998)

      Heterozygosity or homozygosity for vnddd-4 or vnL6 enhances the spi larval mutant phenotype of Df(2L)OD12/Df(2L)VA17 or spi1 mutants. Embryos collapse and extrude the head skeleton. Almost all ventral denticles are lacking.

      (Schnepp et al., 1996)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      vnL6/vnddd-4 is rescued by Scer\GAL4αTub84B.PZ/vnUAS.cSa

      (Zecca and Struhl, 2002)
      Comments

      vnL6/vnddd-4 wing discs containing multiple clones expressing vnScer\UAS.cSa under the control of Scer\GAL4αTub84B.PZ appear similar in size to wild-type discs. Single clones expressing vnScer\UAS.cSa under the control of Scer\GAL4αTub84B.PZ can confer at least partial and sometimes extensive rescue of vnL6/vnddd-4 wing discs. Rescue is only seen when the clones are located in or near the prospective dorsal region of the disc.

      (Zecca and Struhl, 2002)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Shearn.

      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (8)
      References (12)