FlyBase Allele Report: Dmel\en[59]

General Information

Symbol

Dmel\en⁵⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0003774

Feature type

allele

Associated gene

Dmel\en

Associated Insertion(s)

Carried in Construct

Also Known As

en^IO, en^IO34, en^I034

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Condie and Brower, 1989)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Condie and Brower, 1989)

Description

29bp deletion around codon 72 causing a frameshift. 41 amino acids are added before a termination codon occurs at codon 113.

(Gustavson et al., 1996)

Deletion of sequences including coding sequence.

(Eaton and Kornberg, 1990)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:11,527,429..11,527,457 [-]

Comment:

Approximate location of a 29bp deletion that begins in the codon for amino acid 72. The deletion causes a frameshift and early translation termination after the addition of 41 additional residues. The exact position of the deletion was not reported (FBrf0086432).

(Gustavson et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal

(Condie and Brower, 1989, Lawrence and Johnston, 1984, Lawrence and Struhl, 1982)

lethal (with en^Adv-1)

(Srivastava et al., 2002)

lethal | recessive

(Locke and Hanna, 1996)

visible

(Campbell and Tomlinson, 2000)

Phenotype Manifest In

anterior-posterior compartment boundary of the wing blade | somatic clone

(Lawrence and Struhl, 1982)

central nervous system | somatic clone

(Lawrence and Johnston, 1984)

cercus | somatic clone

(Lawrence and Struhl, 1982)

embryonic/larval cuticle

(Bejsovec and Martinez Arias, 1991)

embryonic segment posterior compartment

(Eaton and Kornberg, 1990)

epandrial ventral lobe | somatic clone

(Lawrence and Struhl, 1982)

epidermis | posterior | somatic clone

(Lawrence and Johnston, 1984)

hypandrium | somatic clone

(Lawrence and Struhl, 1982)

imaginal disc posterior compartment

(Eaton and Kornberg, 1990)

Keilin organ

(Simcox et al., 1989)

leg | posterior | somatic clone

(Lawrence and Struhl, 1982)

male genitalia | cell non-autonomous | somatic clone

(Lawrence and Struhl, 1982)

proboscis | posterior | somatic clone

(Lawrence and Struhl, 1982)

surstylus | somatic clone

(Lawrence and Struhl, 1982)

ventral denticle belt

(Bejsovec and Martinez Arias, 1991)

wing | posterior | somatic clone

(Lawrence and Struhl, 1982)

wing vein L4

(Campbell and Tomlinson, 2000)

Detailed Description

Statement

Reference

Lethality is not 100% in en^Adv-1/en⁵⁹ animals - occasional adult escapers are seen.

(Srivastava et al., 2002)

Heterozygotes occasionally (less than 1%) have gaps in wing vein L4.

(Campbell and Tomlinson, 2000)

Strong allele.

(Gustavson et al., 1996)

Hemizygous en⁵⁹ embryos heterozygous for wg^l-12 have a more severe ventral cuticle pattern phenotype than embryos hemizygous for en⁵⁹ alone.

(Bejsovec and Martinez Arias, 1991)

ci^D expression is derepressed in the posterior compartments of embryos and imaginal discs.

(Eaton and Kornberg, 1990)

en¹/en⁵⁹ transheterozygotes have a variable phenotype. About one quarter are nearly indistinguishable from wild-type, while the rest have interruptions of varying extent in the fourth vein. en²/en⁵⁹ transheterozygotes survive to larvae or pharate adults in a few cases. The pharate adults have severe abnormalities in distal segments of all legs and extreme posterior abnormalities in the wing.

(Condie and Brower, 1989)

en mutant embryos lack all Keilin's organs. en⁵⁹/en⁵⁷ embryos grew in in vivo culture, producing implants containing imaginal discs and all larval tissues. Metamorphosed implants were characteristic of eye-antenna discs.

(Simcox et al., 1989)

Homozygous en⁵⁹ cells in mosaic animals made by nuclear transplantation do not develop normally in posterior parts of the epidermis and some regions of the CNS, but form normal patterns in mesodermal and endodermal organs.

(Lawrence and Johnston, 1984)

Posterior homozygous clones in the proboscis are often associated with abnormal bristle patterns. Clones in the posterior part of the wing are abnormal: the wing is enlarged and the posterior margin has large socketed bristles and a well-developed triple row in the clone. Posterior clones do not respect the anteroposterior compartment boundary in the wing. Posterior clones in the leg are often grossly enlarged with a huge increase in the number of bristles. In the male, clones in the genitalia are often associated with missing parts of the claspers, lateral plate and hypandrium, and clones in the analia form unusually shaped anal plates and are often associated with missing genital parts.

(Lawrence and Struhl, 1982)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

en⁵⁹ has visible phenotype, enhanceable by cg[+]/cg⁰⁷⁶⁵⁹

(Campbell and Tomlinson, 2000)

Other

Statement

Reference

cg^Y4, en[+]/en⁵⁹ has visible | dominant phenotype

(Campbell and Tomlinson, 2000)

cg^Y4, en⁵⁹ has visible | dominant phenotype

(Campbell and Tomlinson, 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

en⁵⁹ has wing vein L4 phenotype, enhanceable by cg[+]/cg⁰⁷⁶⁵⁹

(Campbell and Tomlinson, 2000)

Enhancer of

Statement

Reference

en⁵⁹ is an enhancer of phenotype of ci¹

(Locke and Hanna, 1996)

en⁵⁹ is an enhancer of phenotype of ci⁵⁷

(Locke and Hanna, 1996)

en⁵⁹ is an enhancer of phenotype of ci³⁶

(Locke and Hanna, 1996)

Other

Statement

Reference

cg^Y4, en[+]/en⁵⁹ has wing vein L4 phenotype

(Campbell and Tomlinson, 2000)

cg^Y4, en⁵⁹ has wing vein L4 phenotype

(Campbell and Tomlinson, 2000)

Additional Comments

Genetic Interactions

Statement

Reference

25% of en⁵⁹ cg⁰⁷⁶⁵⁹ double heterozygotes have gaps in wing vein L4. cg^Y4 en⁵⁹ double heterozygotes have a fully penetrant loss of wing vein L4 phenotype. en⁵⁹ cg^A22 double heterozygotes are indistinguishable from en⁵⁹/+ flies with regard to wing vein L4.

(Campbell and Tomlinson, 2000)

In ptc⁹ en⁵⁹ double mutants denticle belts appear broader and mirror image symmetry is not found.

(Hidalgo, 1991)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

en^nst-101

(Bhojwani et al., 1995)

en^nst-120

(Bhojwani et al., 1995)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

5343

en⁵⁹/CyO; P{ftz-lacC}1

Notes on Origin

Discoverer

Comments

Complements: tou¹.

(Fauvarque et al., 2001)

Strong en allele.

(Cadigan et al., 1994)

en mutants can be ranked by strength regarding their ability to induce a ci phenotype: en¹ <= Df(2R)en30 <= en⁴ <= en^Enci <= Df(2R)en-SFX31 <= en⁵⁹.

(Locke and Hanna, 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

en⁵⁹

(Bailetti et al., 2019, Seibert and Urbach, 2010)

en^I034

(Locke and Hanna, 1996, Bhojwani et al., 1995)

en^IIO

(Coulter and Wieschaus, 1988)

en^IL034

(Fusse and Hoch, 2002)

en^IO34

(Gustavson et al., 1996, Strutt and Mlodzik, 1996, Eaton and Kornberg, 1990, Condie and Brower, 1989, Tremml and Bienz, 1989)

en^IO

(Alexandre and Vincent, 2003, Simmonds and Bell, 1998, Cadigan et al., 1994, van den Heuvel et al., 1993, Bejsovec and Martinez Arias, 1991, Hidalgo, 1991, Condie and Brower, 1989, Simcox et al., 1989, Tearle and Nusslein-Volhard, 1987, Lawrence and Johnston, 1984, Morata et al., 1983, Lawrence and Struhl, 1982)

Name Synonyms

Secondary FlyBase IDs

References (26)

Report Sections

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