FlyBase Allele Report: Dmel\mst[2]

General Information

Symbol

Dmel\mst²

Species

D. melanogaster

Name

FlyBase ID

FBal0006336

Feature type

allele

Associated gene

Dmel\mst

Associated Insertion(s)

Carried in Construct

Also Known As

LB20, mst^LB20

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Mottier-Pavie et al., 2011)

loss of function allele

(Miklos et al., 1997)

Mutagen

ethyl methanesulfonate

(Perrimon et al., 1989, Schalet and Lefevre, 1973)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C544T.

(Mottier-Pavie et al., 2011)

Amino acid replacement: ?182term.

(Mottier-Pavie et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:21,389,610..21,389,610 [+]

Nucleotide change:

C21389610T

Reported nucleotide change:

C544T

Amino acid change:

R182term | mst-PA; R182term | mst-PB

Reported amino acid change:

?182term

(Mottier-Pavie et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

mitotic spindle

(Mottier-Pavie et al., 2011)

neuroblast | larval stage

(Mottier-Pavie et al., 2011)

Detailed Description

Statement

Reference

The brains of hemizygous larvae show a metaphase arrest phenotype characterised by a high mitotic index, extremely condensed metaphase chromosomes, the almost complete absence of anaphases and an elevated frequency of polyploid cells. 76% of the mutant neuroblasts contain monopolar spindles. Microtubule density in the mutant spindles appears lower than in wild type.

(Mottier-Pavie et al., 2011)

Germ cells are unable to survive in germ line clones. Homozygous embryos do not exhibit any central or peripheral nervous system defects. Larvae have very little imaginal disc tissue and significantly reduced neural tissue. More than 60% of the cells have aneuploid or polyploid chromosome complements. Cytokinesis is normal.

(Miklos et al., 1997)

Lethality occurs during larval or pupal stages. Homozygous germline clones display cell lethality. Embryos derived from heterozygous mothers do not have CNS or PNS defects.

(Perrimon et al., 1989)

larval/pupal lethal

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

mst², rod¹¹⁰⁰ has abnormal mitotic cell cycle | larval stage phenotype

(Mottier-Pavie et al., 2011)

Sas-4^unspecified, mst² has abnormal mitotic cell cycle | larval stage phenotype

(Mottier-Pavie et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

mst² rod¹¹⁰⁰ double mutant larvae show a higher frequency of ana-telophases in the brain than mst² single mutants. The ana-telophase figures of the double mutants show chromosome segregation defects that are much more severe than those seen in rod¹¹⁰⁰ single mutants.

The brains of mst² Sas-4^unspecified double mutant larvae have a very high mitotic index, with most dividing cells arrested in metaphase with highly contracted chromosomes. More than 95% of metaphases lack centrosomes and these metaphases have very peculiar 'spindles': 34% are devoid of microtubules arrays, 4% have small and disorganised bipolar spindles and 62% have acentrosomal microtubule arrays (AMTAs) resembling monopolar spindles. The AMTAs are always associated with metaphase chromosomes, with some microtubules terminating at the chromosomes, and others extending beyond them.

(Mottier-Pavie et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mst² is rescued by mst^+t11.6

(Miklos et al., 1997)

mst² is rescued by mst^+t10.9

(Miklos et al., 1997)

Not rescued by

mst² is not rescued by mst^tMb

(Miklos et al., 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6886

m^s19 mst²/FM6

Notes on Origin

Discoverer

Baldwin.

Comments

Complements: l(1)19Ec^17-344.

(Munoz, 2004.1.5)

Maternal germline clonal analysis demonstrates cell lethality in homozygous clones.

(Perrimon et al., 1989)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

LB20

(Miklos et al., 1997, Russell et al., 1992, Perrimon et al., 1989, Kramers et al., 1983, Lefevre, 1981, Schalet and Lefevre, 1973)

l(1)19Fg²

l(1)19Fg^LB20

(Perrimon et al., 1989)

l(1)LB20

(Munoz, 2004.1.5, Munoz, 2004.1.5, Munoz, 2003.12.10, Miklos et al., 1986)

mst²

mst^LB20

(Baumbach et al., 2015, Palumbo et al., 2015, Mottier-Pavie et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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