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General Information
Symbol
Dmel\mst2
Species
D. melanogaster
Name
FlyBase ID
FBal0006336
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
LB20, mstLB20
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C21389610T

Reported nucleotide change:

C544T

Amino acid change:

R182term | mst-PA; R182term | mst-PB

Reported amino acid change:

?182term

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide substitution: C544T.

Amino acid replacement: ?182term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The brains of hemizygous larvae show a metaphase arrest phenotype characterised by a high mitotic index, extremely condensed metaphase chromosomes, the almost complete absence of anaphases and an elevated frequency of polyploid cells. 76% of the mutant neuroblasts contain monopolar spindles. Microtubule density in the mutant spindles appears lower than in wild type.

Germ cells are unable to survive in germ line clones. Homozygous embryos do not exhibit any central or peripheral nervous system defects. Larvae have very little imaginal disc tissue and significantly reduced neural tissue. More than 60% of the cells have aneuploid or polyploid chromosome complements. Cytokinesis is normal.

Lethality occurs during larval or pupal stages. Homozygous germline clones display cell lethality. Embryos derived from heterozygous mothers do not have CNS or PNS defects.

larval/pupal lethal

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

mst2 rod1100 double mutant larvae show a higher frequency of ana-telophases in the brain than mst2 single mutants. The ana-telophase figures of the double mutants show chromosome segregation defects that are much more severe than those seen in rod1100 single mutants.

The brains of mst2 Sas-4unspecified double mutant larvae have a very high mitotic index, with most dividing cells arrested in metaphase with highly contracted chromosomes. More than 95% of metaphases lack centrosomes and these metaphases have very peculiar 'spindles': 34% are devoid of microtubules arrays, 4% have small and disorganised bipolar spindles and 62% have acentrosomal microtubule arrays (AMTAs) resembling monopolar spindles. The AMTAs are always associated with metaphase chromosomes, with some microtubules terminating at the chromosomes, and others extending beyond them.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Not rescued by

mst2 is not rescued by msttMb

Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

Baldwin.

Comments
Comments

Maternal germline clonal analysis demonstrates cell lethality in homozygous clones.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (14)