The majority of intact wing imaginal discs cultured in vivo at 15^oC remain morphologically normal but show no significant growth. The majority of intact discs grown at 29^oC become abnormal and show signs of growth. Culture of amputated discs at 15^oC generates abnormal discs. Gamma irradiated discs (whole or amputated) become abnormal after in vivo culture at 15^oC but show no significant growth. l(2)gl^ts1/l(2)gl^ts3 heteroallelic females exhibit normal morphology of ovaries and developing egg chambers. Heterozygous females lay normal eggs at 22^oC and 29^oC. At 29^oC the eggs fail to develop into viable larvae. None of the unhatched eggs show differentiated larval cuticles. Embryos stop developing at the stage of germ band formation, none of the embryos exhibit dorsal closure and differentiation of the larval hypodermis. None of the embryos have developed a well defined CNS.

Viability of heterozygotes with l(2)gl^ts3 is temperature-dependent, none of the progeny emerged from a cross with l(2)gl^ts2 at 29^oC. At 22^oC heteroallelic females produce eggs that develop into normal embryos, at 29^oC eggs with normal morphology are produced but they fail to hatch into viable larvae.

Lethality of heterozygotes with Df(2L)net62 when raised at 29^oC is complete.

Shows 100% lethality when heterozygous with l(2)gl⁴ or Df(2L)net62 at 29^oC. Does not adversely effect larval viability at either 29^oC or 15^oC. Hemizygous larvae form a puparium at 29^oC, however the majority of larvae fail to evert their imaginal discs and only 3% of larvae differentiate adult cuticle structures. At 15^oC, 96% of larvae evert their imaginal discs and 41% of larvae differentiate adult cuticle structures. Imaginal discs are morphologically abnormal in larvae raised at 29^oC; approximately 60% of wing discs and 38% of haltere discs are abnormal, and some abnormal first and third leg discs are seen. The proximal region of the wing discs is thickened and has lost its normal folding pattern, while the distal region is normal in shape and has folds. The abnormal regions contains cells arranged in multilayer clumps and chords. Wing discs raised at 29^oC and injected into wild-type larvae produce undifferentiated tissue in addition to differentiated tissue and residual material. The undifferentiated tissue is neoplastic when placed in adult hosts at 29^oC.

Homozygous brain hemispheres injected into adult hosts remain normal at 22^oC and 29^oC. Homozygous larvae raised at 29^oC contain both morphologically normal and abnormal wing discs. Discs of both types cultured in adult hosts at 29^oC give rise to transplantable neoplastic tissue. When discs raised at 29^oC are cultured in adult host at 15^oC, the morphologically normal discs maintain their normal morphology, but the morphologically abnormal discs give rise to neoplasms. Homozygous larvae raised at 15^oC have morphologically normal wing discs, which maintain their normal morphology when cultured in adult hosts at 15^oC, but give rise to neoplasms when cultured in adult hosts at 29^oC. Homozygous wing discs raised at 15^oC, cut into two fragments and cultured in adult hosts at 15^oC become neoplastic.

Df(2L)net62/l(2)gl^ts1 has lethal | heat sensitive phenotype, suppressible by Dpse\l(2)gl^+t12.2

l(2)gl^ts1 has lethal phenotype, suppressible by Dpse\l(2)gl^+t12.2