FlyBase Allele Report: Dmel\l(2)gl[ts3]

When l(2)gl⁴/l(2)gl^ts3 flies are cultivated at 27.5[o]C, the temperature-sensitive l(2)gl^ts3 partially loses its activity, and 86% of individual flies successfully develop into adulthood. Approximately 28% of l(2)gl⁴/l(2)gl^ts3 flies carry at least one defective extrasensory bristle, such as a duplicated bristle or a socket without a bristle, as a result of the partial loss of l(2)gl at the semi-restrictive temperature of 27.5[o]C.

(Ogawa et al., 2009)

In l(2)gl⁴/l(2)gl^ts3 flies the border follicle cells have much reduced polarity. Delamination of these cells is delayed, but their migration is accelerated.

(Szafranski and Goode, 2004)

The polarity of the dorsal leading edge cells of the embryonic epidermis is disrupted, though other ectodermal cells are normal. Embryos reared at 29^oC show failures of dorsal closure and failure of head involution. Cephalopharyngeal skeleton and filzkorper can be absent or not externalized, respectively. 58% of cuticles show a dorsal hole. Denticle belts are extended, reaching up to 60% of lateral extension in some mutant individuals. Rare embryos show the phenotype of partly lacking ventral epidermis. Less than 8% of cuticles show patches of denticle belt fusion or naked cuticle, indicating that on the whole wg and hh signaling are unaffected.

(Arquier et al., 2001)

Exposure to 29^oC during external sensory organ development causes transformation of inner cells to outer cells causing double socket and hair phenotypes.

(Ohshiro et al., 2000)

Ovaries of l(2)gl^ts3 females shifted to 29^oC immediately after hatching and reared at 29^oC for 2 days are severely reduced in size, due to a lack of late stage egg chambers.

(de Lorenzo et al., 1999)

The majority of intact wing imaginal discs cultured in vivo at 15^oC remain morphologically normal but show no significant growth. The majority of intact discs grown at 29^oC become abnormal and show signs of growth. Culture of amputated discs at 15^oC generates abnormal discs. Gamma irradiated discs (whole or amputated) become abnormal after in vivo culture at 15^oC but show no significant growth. l(2)gl^ts1/l(2)gl^ts3 and l(2)gl^ts2/l(2)gl^ts3 heteroallelic females exhibit normal morphology of ovaries and developing egg chambers. Heterozygous females lay normal eggs at 22^oC and 29^oC. At 29^oC the eggs fail to develop into viable larvae. None of the unhatched eggs show differentiated larval cuticles. Embryos stop developing at the stage of germ band formation, none of the embryos exhibit dorsal closure and differentiation of the larval hypodermis. None of the embryos have developed a well defined CNS.

(Baek and Hanratty, 1997)

Viability of heterozygotes with l(2)gl^ts1 and l(2)gl^ts2 is temperature-dependent. At 22^oC heteroallelic females produce eggs that develop into normal embryos, at 29^oC eggs with normal morphology are produced but they fail to hatch into viable larvae.

(Baek and Hanratty, 1996)

Lethality of heterozygotes with Df(2L)net62 when raised at 29^oC is complete. Homozygous mutant larvae produced at 29^oC pupariate on a normal timetable and die during the early pupal stages. Male homozygotes and heterozygotes with l(2)gl^ts1 or l(2)gl^ts2 show male sterility when raised at 27^oC. Genitalia are incompletely rotated, disorganized and protruding. Mutant embryos from mutant mothers show developmental block at germ band shortening. Head involution fails but segmentation progresses normally. Cell shape changes at the heading edge of the dorsal epidermis fail to occur, reminiscent of zip and scb mutant phenotypes. Midgut is hypertrophied and the yolk remains incompletely digested. The visceral mesoderm is apparently normal. Midgut cells arrest their differentiation early. Causes a temperature sensitive reduction in female fertility. Oogenesis blocks at stage 7-8 in females raised at 29^oC for 6 days. Blocked egg chambers show a multilayered accumulation of probably follicular cells at the anterior and posterior tips. These cells become internalized into the space normally occupied by the ooplasm. The germarium becomes fused with the youngest egg chambers.

(Manfruelli et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

l(2)gl^ts3 has lethal phenotype, suppressible by Dpse\l(2)gl^+t12.2

(Manfruelli et al., 1996)

l(2)gl^ts3/Df(2L)net62 has lethal | heat sensitive phenotype, suppressible by Dpse\l(2)gl^+t12.2

(Manfruelli et al., 1996)

Suppressor of

Statement

Reference

l(2)gl^ts3/l(2)gl[+] is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Ankle2^A

(Link et al., 2019)

l(2)gl^ts3/l(2)gl[+] is a suppressor | partially of increased mortality during development | temperature conditional phenotype of Ankle2^A

(Link et al., 2019)

l(2)gl^ts3/l(2)gl[+] is a suppressor of abnormal size | third instar larval stage phenotype of Scer\GAL4^Act5C.PI, ZIKV\NS4A^UAS.Tag:HA

(Link et al., 2019)

l(2)gl^ts3/l(2)gl[+] is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^Act5C.PI, ZIKV\NS4A^UAS.Tag:HA

(Link et al., 2019)

Other

Statement

Reference

Ankle2^A, l(2)gl^ts3/l(2)gl[+] has partially lethal - majority die phenotype

(Link et al., 2019)

Ankle2^A, l(2)gl^ts3/l(2)gl[+] has short lived phenotype

(Link et al., 2019)

l(2)gl^ts3/l(2)gl⁴, mts⁰²⁴⁹⁶ has abnormal eclosion rhythm phenotype

(Ogawa et al., 2009)

l(2)gl^ts3/l(2)gl⁴, mts^XE-2258 has abnormal eclosion rhythm phenotype

(Ogawa et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

l(2)gl^ts3/l(2)gl⁴ has mechanosensory chaeta | heat sensitive phenotype, enhanceable by mts⁰²⁴⁹⁶

(Ogawa et al., 2009)

l(2)gl^ts3/l(2)gl⁴ has mechanosensory chaeta | heat sensitive phenotype, enhanceable by mts^XE-2258

(Ogawa et al., 2009)

l(2)gl^ts3 has eo neuron phenotype, enhanceable by numb[+]/numb²

(Ohshiro et al., 2000)

l(2)gl^ts3 has tormogen cell phenotype, enhanceable by numb[+]/numb²

(Ohshiro et al., 2000)

l(2)gl^ts3 has external sensory organ phenotype, enhanceable by numb[+]/numb²

(Ohshiro et al., 2000)

Suppressor of

Statement

Reference

l(2)gl^ts3/l(2)gl[+] is a suppressor | partially of larval brain | third instar larval stage phenotype of Ankle2^A

(Link et al., 2019)

l(2)gl^ts3/l(2)gl[+] is a suppressor | partially of larval brain | third instar larval stage phenotype of Scer\GAL4^Act5C.PI, ZIKV\NS4A^UAS.Tag:HA

(Link et al., 2019)

l(2)gl^ts3 is a suppressor of embryonic epidermis phenotype of Scer\GAL4^en-e16E, dpp^UAS.cSa

(Arquier et al., 2001)

l(2)gl^ts3 is a suppressor of embryonic epidermis phenotype of Scer\GAL4^prd.RG1, dpp^UAS.cSa

(Arquier et al., 2001)

NOT Suppressor of

Statement

Reference

l(2)gl^ts3 is a non-suppressor of embryonic epidermis phenotype of Scer\GAL4^prd.RG1, tkv^{Q253D.UAS.cNb}

(Arquier et al., 2001)

Other

Statement

Reference

l(2)gl^ts3, numb^hs.PU has external sensory organ phenotype

(Ohshiro et al., 2000)

l(2)gl^ts3, numb^hs.PU has tormogen cell phenotype

(Ohshiro et al., 2000)

l(2)gl^ts3, numb^hs.PU has eo neuron phenotype

(Ohshiro et al., 2000)

ecd¹, l(2)gl^ts3 has wing disc phenotype

(Manfruelli et al., 1996)

ecd¹, l(2)gl^ts3 has larva phenotype

(Manfruelli et al., 1996)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of one copy of mts⁰²⁴⁹⁶ or mts^XE-2258 to l(2)gl⁴/l(2)gl^ts3 flies reduces the eclosion rate to 72% and 42% respectively.

The presence of one copy of mts⁰²⁴⁹⁶ or mts^XE-2258 in l(2)gl⁴/l(2)gl^ts3 flies increases the frequency of defective bristle formation to 33% and 64% respectively (compared to 28% in l(2)gl⁴/l(2)gl^ts3 mutants).

(Ogawa et al., 2009)

Double mutants with ecd¹ show a delay in larval life and the formation of bloated and giant larvae, similar to the phenotype of the l(2)gl null mutants. Wing imaginal discs show neoplastic transformations.

(Manfruelli et al., 1996)

Xenogenetic Interactions

Statement

Reference

Lethality with Df(2L)net62 can be completely rescued by Dpse\l(2)gl^+t12.2. Neither giant larvae nor tumorous brain\imaginal discs are observed.

(Manfruelli et al., 1996)

Complementation and Rescue Data

Partially complements

l(2)gl^ts2

(Manfruelli et al., 1996)

l(2)gl^ts1

(Manfruelli et al., 1996)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

36288

y¹ w^*; l(2)gl^ts3 cn¹ speck¹/CyO, y⁺

Notes on Origin

Discoverer

Baek and Hanratty

(Baek and Hanratty, 1996)

Comments

l(2)gl^ts3 has been used to show that the l(2)gl product is required both during embryonic (stage 8-12) and larval (third instar) life.

(Manfruelli et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

l(2)gl^ts3

(Link et al., 2019)

l(2)glts3

(Arquier and Semeriva, 1998)

lgl^ts3

(Moreira et al., 2019, Ogawa et al., 2009, Wirtz-Peitz et al., 2008, Wirtz-Peitz et al., 2008, Bardin et al., 2004, Arquier et al., 2001, Ohshiro et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

Open Close