FlyBase Allele Report: Dmel\numb[hs.PU]

General Information

Symbol

Dmel\numb^hs.PU

Species

D. melanogaster

Name

heat shock construct of Uemura

FlyBase ID

FBal0035308

Feature type

allele

Associated gene

Dmel\numb

Associated Insertion(s)

Carried in Construct

P{hs-numb}

Also Known As

hs-numb

Key Links

Transgenic product class

cDNA

(Rhyu et al., 1994)

wild_type

(Rhyu et al., 1994)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Rhyu et al., 1994)

wild_type

(Rhyu et al., 1994)

Mutagen

in vitro construct

(Rhyu et al., 1994)

Progenitor genotype

Carried in construct

P{hs-numb}

Cytology

Description

Construct: numb cDNA expressed from an Hsp70 promoter.

(Rhyu et al., 1994)

Allele components

Component

Use(s)

Encoded product / tool

numb

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | heat sensitive

(Duan et al., 2011)

Phenotype Manifest In

chaeta | heat sensitive

(Duan et al., 2011)

chemosensory sensory organ & wing vein L1 & glial cell | supernumerary

(van de Bor and Giangrande, 2001)

chemosensory sensory organ & wing vein L3 & glial cell | supernumerary

(van de Bor and Giangrande, 2001)

chordotonal neuron

(Rhyu et al., 1994)

chordotonal organ

(Rhyu et al., 1994)

dorsal row of wing sensilla

(Rhyu et al., 1994)

eo neuron

(van de Bor and Giangrande, 2001, Rhyu et al., 1994)

external sensory organ | embryonic stage

(Rhyu et al., 1994)

interommatidial bristle

(Rhyu et al., 1994)

macrochaeta

(Rhyu et al., 1994)

mechanosensory sensory organ

(van de Bor and Giangrande, 2001)

mechanosensory sensory organ & wing vein L1 & glial cell | supernumerary

(van de Bor and Giangrande, 2001)

mechanosensory sensory organ & wing vein L3 & glial cell | supernumerary

(van de Bor and Giangrande, 2001)

microchaeta

(Rhyu et al., 1994)

postorbital bristle | heat sensitive

(Berdnik et al., 2002)

scolopale cell

(Rhyu et al., 1994)

scolopidial dendritic cap cell

(Rhyu et al., 1994)

thecogen cell

(van de Bor and Giangrande, 2001, Rhyu et al., 1994)

tormogen cell

(van de Bor and Giangrande, 2001, Guo et al., 1996, Rhyu et al., 1994)

trichogen cell

(van de Bor and Giangrande, 2001, Rhyu et al., 1994)

ventral row of wing sensilla

(Rhyu et al., 1994)

vMP2 neuron

(Spana et al., 1995)

Detailed Description

Statement

Reference

Expression of numb^hs.PU using heat shock at 14 hours after puparium formation (during the division of the microchaeta sensory organ precursor cells) results in extensive bristle loss on the notum.

(Duan et al., 2011)

numb^hs.PUm flies heat shocked as pupae (2x 45 minute 37^oC heat shocks separated by 30, at 16-18 hours APF) exhibit loss of approximately 70% of postorbital bristles.

(Berdnik et al., 2002)

Phenotype resembles that of N^l1N-ts1. A one hour heat shock at the time of the birth of the sensory organ precursors causes an increase in glial cells in most lineages analyzed. The most extreme phenotype is sensory organs with all six cells being glial cells.

(van de Bor and Giangrande, 2001)

Transient shifts to 29^oC during external sensory organ development do not perturb the formation of normal sensory cell clusters containing a neuron and a socket cell. However in a l(2)gl^ts3 background, outer cells (such as sockets) become transformed to inner cells (such as neurons) with a consequent loss of bristles on the notum.

(Ohshiro et al., 2000)

Heat shock during larval development causes the development of double hairs. Without heat shock flies exhibit no bristle phenotype.

(Guo et al., 1996)

Ectopic expression of numb is sufficient to transform vMP2 into the dMP2 cell fate, the transformed cell has odd expression and has a posterior axon projection. The physical asymmetry of the MP2 division is not altered, only the fate of the daughter cells.

(Spana et al., 1995)

After heat shock at 39^oC during the time when the sensory organ precursors divide (5-9hrs of development at 25^oC), embryos develop transformed external sensory organs, with a duplicated neuron with associated sheath cell, formed at the expense of the outer hair and socket support cells. Heat shock during microchaetae formation in the pupa causes both balding and twinning of bristles. Transformations to neurons underlie the balding effect, which occurs with earlier heat shocks. Transformation of socket cell to hair cell underlies the twinning effect which occurs with later heat shocks.

(Rhyu et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

numb^hs.PU has visible | heat sensitive phenotype, suppressible by insv^unspecified

(Duan et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

numb^hs.PU has chaeta | heat sensitive phenotype, suppressible by insv^unspecified

(Duan et al., 2011)

NOT Suppressor of

Statement

Reference

numb^hs.PU is a non-suppressor of tormogen cell | ectopic | somatic clone phenotype of AP-2α^ear4

(Berdnik et al., 2002)

numb^hs.PU is a non-suppressor of macrochaeta | somatic clone phenotype of AP-2α^ear4

(Berdnik et al., 2002)

Other

Statement

Reference

l(2)gl^ts3, numb^hs.PU has external sensory organ phenotype

(Ohshiro et al., 2000)

l(2)gl^ts3, numb^hs.PU has tormogen cell phenotype

(Ohshiro et al., 2000)

l(2)gl^ts3, numb^hs.PU has eo neuron phenotype

(Ohshiro et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The bristle loss on the notum caused by expression of numb^hs.PU using heat shock at 14 hours after puparium formation is strongly suppressed by insv^unspecified.

(Duan et al., 2011)

The bristle transformations seen in α-Adaptin^ear4 clones are unaffected by the presence of numb^hs.PU and heat shock conditions (2x 45 minute 37^oC heat shocks separated by 30, at 16-18 hours APF).

(Berdnik et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

T. Uemura.

(Rhyu et al., 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

numb^hs.PU

Name Synonyms

heat shock construct of Uemura

Secondary FlyBase IDs

References (8)

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