The majority of intact wing imaginal discs cultured in vivo at 15^oC remain morphologically normal but show no significant growth. The majority of intact discs grown at 29^oC become abnormal and show signs of growth. Culture of amputated discs at 15^oC generates abnormal discs. Gamma irradiated discs (whole or amputated) become abnormal after in vivo culture at 15^oC but show no significant growth. l(2)gl^ts2/l(2)gl^ts3 heteroallelic females exhibit normal morphology of ovaries and developing egg chambers. Heterozygous females lay normal eggs at 22^oC and 29^oC. At 29^oC the eggs fail to develop into viable larvae. None of the unhatched eggs show differentiated larval cuticles. Embryos stop developing at the stage of germ band formation, none of the embryos exhibit dorsal closure and differentiation of the larval hypodermis. None of the embryos have developed a well defined CNS.

Viability of heterozygotes with l(2)gl^ts3 is temperature-dependent, none of the progeny emerged from a cross with l(2)gl^ts1 at 29^oC. At 22^oC heteroallelic females produce eggs that develop into normal embryos, at 29^oC eggs with normal morphology are produced but they fail to hatch into viable larvae.

Lethality of heterozygotes with Df(2L)net62 when raised at 29^oC is complete.

Shows 100% lethality when heterozygous with l(2)gl⁴ or Df(2L)net62 at 29^oC. Larval viability is significantly reduced at 29^oC or 15^oC. Hemizygous larvae form a puparium at 29^oC, however 90% of larvae fail to evert their imaginal discs and only 7% of larvae differentiate adult cuticle structures. At 15^oC, 55% of larvae evert their imaginal discs and 14% of larvae differentiate adult cuticle structures.

l(2)gl^ts2/Df(2L)net62 has lethal | heat sensitive phenotype, suppressible by Dpse\l(2)gl^+t12.2

l(2)gl^ts2 has lethal phenotype, suppressible by Dpse\l(2)gl^+t12.2