FlyBase Allele Report: Dmel\mle[9]

General Information

Symbol

Dmel\mle⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0012342

Feature type

allele

Associated gene

Dmel\mle

Associated Insertion(s)

Carried in Construct

Also Known As

mle^γ38

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

gamma ray

Nature of the Allele

Allele class

loss of function allele

(Hilfiker et al., 1994)

Mutagen

gamma ray

(Kernan et al., 1991)

Progenitor genotype

Cytology

Description

In addition to the previously reported deletion in the catalytic core of mle, the mle⁹ mutant also contains a 21bp deletion that results in the loss of 7 amino acids from the C-terminal RGG-box domain (the first of two GGGYGNN repeats immediately downstream of the nuclear localization signal is lost).

(Zolin et al., 2025)

49bp deletion that removes 8bp of the last intron and 41bp of the beginning of the last exon of mle. The deletion removes the 3' splice site of the last intron, and mRNA analysis indicates that splicing uses the first alternative AG site, shifting the beginning of the last exon, and resulting in a deletion of 72bp from the last exon in mle mRNAs expressed in the mutant. This results in the deletion of 24 amino acids (536-559 of the RA isoform) from the expressed protein, removing sequence encoding the conserved motif III of the first RecA-like domain.

(Ashniev et al., 2024)

Deletion in mle.

(Kernan et al., 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:5,977,428..5,977,476 [-]

Comment:

A 49bp in mle. First of two deletions in mle in the mutant.

(Ashniev et al., 2024)

deletion

2R:5,975,448..5,975,468 [-]

Comment:

A 21bp deletion that results in the loss of 7 amino acids from the C-terminal RGG-box domain (one of two GGGYGNN repeats immediately downstream of the nuclear localization signal is lost). Second of two deletions in mle in the mutant.

(Zolin et al., 2025)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal

(Hilfiker et al., 1994)

lethal | male

(Kernan et al., 1991)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous males show appreciable survival to larval stages (day 5) in the presence of infection with the "male-killing" bacterium S.poulsonii when their mother is also homozygous, while infected heterozygous sibling males show no survival to day 5. Homozygous and heterozygous infected male embryos derived from heterozygous females show no survival to day 5.

(Veneti et al., 2005)

male-lethal

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

mle[+]/mle⁹ is a non-enhancer of visible phenotype of BEAF-32^{BID.UAS.Tag:HA,Tag:NLS(SV40-largeT)}, Scer\GAL4^ey.PH

(Roy et al., 2011)

Suppressor of

Statement

Reference

mle⁹ is a suppressor of lethal | female | larval stage phenotype of vir^2f

(Hilfiker et al., 1995)

NOT Suppressor of

Statement

Reference

mle⁹ is a non-suppressor of lethal | female | larval stage phenotype of vir³

(Hilfiker et al., 1995)

mle⁹ is a non-suppressor of lethal | female | larval stage phenotype of vir⁴

(Hilfiker et al., 1995)

mle⁹ is a non-suppressor of lethal | female | larval stage phenotype of vir⁵

(Hilfiker et al., 1995)

mle⁹ is a non-suppressor of lethal | female | larval stage phenotype of vir⁶

(Hilfiker et al., 1995)

mle⁹ is a non-suppressor of lethal | female | larval stage phenotype of vir⁷

(Hilfiker et al., 1995)

mle⁹ is a non-suppressor of lethal | female phenotype of vir^unspecified

(Hilfiker et al., 1995)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

mle[+]/mle⁹ is a non-enhancer of eye phenotype of BEAF-32^{BID.UAS.Tag:HA,Tag:NLS(SV40-largeT)}, Scer\GAL4^ey.PH

(Roy et al., 2011)

Suppressor of

Statement

Reference

mle⁹ is a suppressor of ovary phenotype of ovo^D1rv22

(Oliver and Pauli, 1998)

Other

Statement

Reference

mle¹⁴/mle⁹, ovo^D1rv22 has ovary phenotype

(Oliver and Pauli, 1998)

mle¹⁴/mle⁹, ovo^D1rv23 has ovary phenotype

(Oliver and Pauli, 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The rough eye phenotype of flies expressing BEAF-32^{BID.Scer\UAS.T:Ivir\HA1,T:SV40\nls2} under the control of Scer\GAL4^ey.PH is not enhanced by mle⁹/+.

(Roy et al., 2011)

Ovaries of homozygous ovo^D1rv22 females contain germline chambers in less than 20% of cases. This phenotype is partially suppressed if the females are also carrying mle⁹/mle⁹. ovo^D1rv23/ovo^D1rv22; mle⁹/mle¹⁴ ovaries are phenotypically mosaic, containing germ cells of both male and female morphology. The mosaicism can be at the level of whole ovaries or ovarioles.

(Oliver and Pauli, 1998)

Female lethality of EMS induced vir mutations cannot be rescued by the mle⁹ mutation.

(Hilfiker et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Mutant phenotype can be rescued by P element mediated transformation of a wild type mle gene copy.

(Kernan et al., 1991)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

5873

mle⁹ cn¹ bw¹/CyO

Notes on Origin

Discoverer

Comments

msl-1 is not associated with the X chromosome of male larvae. Salivary gland chromosomes of flies, with introduction of mle⁹ into vir^ts homozygous female larvae, show no evidence of H4Ac16 binding.

(Hilfiker et al., 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mle⁹

(Roy et al., 2011)

mle⁹

(Zolin et al., 2025, Ashniev et al., 2024, Tikhonova et al., 2024, Titus et al., 2023, McCarthy et al., 2022, Valsecchi et al., 2021, Leatham-Jensen et al., 2019, Kim et al., 2018, Valsecchi et al., 2018, Figueiredo et al., 2014, Spierer et al., 2005)

mle^γ38

(Bai et al., 2004, Hilfiker et al., 1995, Hilfiker et al., 1994, Kernan et al., 1991)

Name Synonyms

Secondary FlyBase IDs

References (19)

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