Female germ cells homozygous for vir3 can complete oogenesis. Females carrying these homozygous germ line clones produce eggs (although fecundity is reduced) but when mated to vir+ males no larvae hatch (except for a few escapers). These phenotypes are rescued if the homozygous germ line clones are induced in females carrying vir+t10. The developmental potential of vir3/+ embryos derived from females carrying homozygous vir3 germ line clones and vir+ males is variable; some arrest at the blastoderm stage, others die as differentiated embryos or, rarely, as larvae. Whole larval gonads of the genotype XY ; vir3/Df(2R)vir130 form sperm after they are transplanted into the abdomen of adult females. Fertile males with integrated vir3/Df(2R)vir130 pole cells can be produced when XY ; vir3/Df(2R)vir130 pole cells are transplanted into agametic XY embryos.
Male homozygotes and male transheterozygotes with other EMS induced vir mutations die during larval or pupal stage, very few male escapers are present. Transheterozygotes with virts transform XX animals into intersexes at 29oC. Homozygous clones that occur in the sexually dimorphic regions of females differentiate male structures. Clones in males produce normal male structures. Sxl+/Sxl+;vir3/virts and Sxlf1/Sxl+;vir3/vir+ are viable but doubly heterozygous Sxlf1/Sxl+;vir3/virts females are lethal.