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General Information
Symbol
Dmel\Ubx195
Species
D. melanogaster
Name
FlyBase ID
FBal0017409
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G16710567A

Reported nucleotide change:

TGG?TAG

Amino acid change:

W288term | Ubx-PA; W271term | Ubx-PC; W262term | Ubx-PD; W279term | Ubx-PE

Reported amino acid change:

W?term

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement. See Figure 2c of FBrf0046283. Nonsense codon only causes termination in transcripts which use this exon. These transcripts should terminate 7 amino acids before the start of the homeobox.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology

Polytene chromosomes normal.

Nature of the lesion
Statement
Reference

Point mutation.

UAG stop codon in mII. Inactivates Ubx isoforms Ia/b and IIa/b, but not IVa.

Mutation causing a stop codon in the third Ubx exon.

Single nucleotide change that introduces a nonsense codon in the exon at -53kb.

Nucleotide substitution: G?A.

Point mutation in the -50kb microexon, which generates a nonsense codon in the open reading frame of transcripts that use this microexon. Proteins encoded by these transcripts should terminate 7 amino acids before the start of the homeobox.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Denticle belts of the third thoracic segment and the first abdominal segment are transformed to resemble those of the second thoracic segment.

Haltere enlarged, average bristle number 0.8 (+-1.2 (SD); N=252).

Transformation of the PNS patterns of parasegment 5 and 6 into copies of that of parasegment 4.

Heterozygotes for Ubx195 and a bxd breakpoint mutation show complete T3p to T2p ("pbx") and A1a to T3a ("bxd") transformations.

Does not affect the frequency of the trx bithorax-variegated phenotype in heterozygous combination with Df(3R)red-P52.

Transvection positive with Ubxbx-34e.

Homozygotes complete embryogenesis and occasionally survive as third instar larvae. They have the characteristic Ubx phenotype of transformed denticle belts and three pairs of anterior spiracles. Ubx195/Ubxabx-2 transheterozygotes show extensive transformations of second thoracic leg to first leg structures. Many Ubx195/Ubxbxd-100 or Ubx195 /Ubxabx-2 flies eclose. Ubx195 in trans with abx or bxd alleles has virtually no effect on the development of the adult nervous system; the pattern of commissures is indistinguishable from wild-type in Ubx195/Ubxbxd-100 adults. In Ubx61d/Ubxbxd-100 larvae A1 is transformed to T3, and a pair of presumptive leg neuromeres is formed in this transformed segment.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference

Ubx195 has phenotype, enhanceable by Hrb27C10280

Ubx195 has phenotype, enhanceable by fl(2)d2

Ubx195 has phenotype, enhanceable by vir3

Ubx195 has haltere sensillum phenotype, enhanceable by crn3

Ubx195 has haltere sensillum phenotype, enhanceable by Df(4)G

Ubx195 has haltere sensillum phenotype, enhanceable by Hrb27C[+]/Hrb27C10280

Ubx195 has haltere sensillum phenotype, enhanceable by crn4

Ubx195 has haltere sensillum phenotype, enhanceable by kz26

NOT Enhanced by
Statement
Reference

Ubx195 has phenotype, non-enhanceable by Df(3R)ry506-85C

Suppressed by
Statement
Reference

Ubx195 has haltere phenotype, suppressible by Df(3R)l26c

NOT suppressed by
Statement
Reference

Ubx195 has phenotype, non-suppressible by Df(3R)ry506-85C

Additional Comments
Genetic Interactions
Statement
Reference

Hrb27C10280 enhances the haltere phenotype of Ubx195/+ such that halteres are enlarged, and average bristle number 3.6 (+-1.6 (SD); N=50).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Lewis, Jan. 1968.

Comments
Comments

The phenotype of a number of combinations of Ubx mutations has been investigated to determine the role of the bxd regulatory region in the modulation of Ubx expression.

Phenotype in the PNS is qualitatively and quantitatively identical to that for complete absence of Ubx function.

External Crossreferences and Linkouts ( 2 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Ubx195
Name Synonyms
Secondary FlyBase IDs
    References (20)