FlyBase Allele Report: Dmel\mnb[1]

General Information

Symbol

Dmel\mnb¹

Species

D. melanogaster

Name

FlyBase ID

FBal0012364

Feature type

allele

Associated gene

Dmel\mnb

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Tejedor et al., 1995)

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Amino acid replacement: A126T.

(Tejedor et al., 1995)

Point mutation within the active site of the kinase which is involved in phosphorylation.

(Zhu et al., 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:17,870,363..17,870,363 [-]

Nucleotide change:

G17870363A

Amino acid change:

Reported amino acid change:

A126T

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement.

(Tejedor et al., 1995)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior

(Tejedor et al., 1995)

abnormal developmental rate

(Tejedor et al., 1995)

abnormal eclosion

(Tejedor et al., 1995)

abnormal neuroanatomy

(Chen et al., 2014)

abnormal neuroanatomy (with Df(1)RR79)

(Chen et al., 2014)

abnormal neurophysiology

(Chen et al., 2014)

abnormal neurophysiology | larval stage

(Han et al., 2023)

abnormal size | adult stage

(Degoutin et al., 2013)

abnormal size | adult stage | dominant

(Degoutin et al., 2013)

abnormal size | larval stage

(Tejedor et al., 1995)

increased cell death | third instar larval stage

(Shaikh et al., 2016)

increased cell number | third instar larval stage

(Shaikh et al., 2016)

increased occurrence of cell division | third instar larval stage

(Shaikh et al., 2016)

partially lethal - majority die (with Df(1)RR79)

(Chen et al., 2014)

viable (with mnb^d419)

(Brown et al., 2024)

Phenotype Manifest In

Detailed Description

Statement

Reference

mnb¹/mnb¹ mutants exhibit a significant increase in proliferation of ganglion cells, but not neuroblasts or ganglion mother cells; and show a significant increase in the number of apoptotic cells in the outer proliferation center and central brain of late third instar larvae, as compared to wild type.

(Shaikh et al., 2016)

mnb¹ mutant NMJs have fewer synaptic boutons than controls, but the remaining boutons are larger in size.

mnb¹/Df(1)RR79 mutant NMJs have fewer synaptic boutons than controls, but the remaining boutons are larger in size.

mnb¹ mutants have increased miniature excitatory postsynaptic potential (mEPSP) amplitude but normal evoked EPSP amplitudes. When the nerve is stimulated at a high frequency (10Hz) for a prolonged period (10 minutes), mnb¹ shows a significantly faster rundown than controls.

mnb¹ mutant larvae exhibit synaptic vesicle endocytosis defects: the level of membrane uptake (dye internalisation) in mutant synaptic boutons in response to nerve stimulation is significantly reduced compared to controls. When dye unloading occurs in response to a second stimulation, the mnb¹ mutants unload a similar amount of dye to controls, suggesting exocytosis is unaffected.

mnb¹/Df(1)RR79 mutant larvae exhibit synaptic vesicle endocytosis defects: the level of membrane uptake (dye internalisation) in mutant synaptic boutons in response to nerve stimulation is significantly reduced compared to controls. When dye unloading occurs in response to a second stimulation, the mnb¹/Df(1)RR79 mutants unload a similar amount of dye to controls, suggesting exocytosis is unaffected.

(Chen et al., 2014)

The tarsal segments of legs from hemizygous mnb¹ mutant flies are 7% smaller than in controls. The wings of homozygous mnb¹ mutant males are 19% smaller than in wild type.

(Degoutin et al., 2013)

Reduced brain volume, neuronal architecture has no gross alterations. External appearance is wild type, body size is slightly smaller than wild type. Mutants require 10% more time for their development and have considerable difficulty escaping from the pupal case. Adults exhibit defects in olfactory and visual behaviour. The neuroblast proliferation centre in larval brains is altered, unable to generate sufficient numbers of optic lobe and central brain neurons.

(Tejedor et al., 1995)

Reduction in fibre number in the anterior optic tracts.

(Hoube and Fischbach, 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mnb¹ has abnormal neuroanatomy phenotype, suppressible | partially by Synj^UAS.Tag:HA/Scer\GAL4^nSyb.PS

(Chen et al., 2014)

mnb¹ has abnormal neurophysiology phenotype, suppressible by Synj^UAS.Tag:HA/Scer\GAL4^nSyb.PS

(Chen et al., 2014)

mnb¹ has abnormal neuroanatomy phenotype, suppressible | partially by Scer\GAL4^nSyb.PS/Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)}

(Chen et al., 2014)

Other

Statement

Reference

mnb¹, rol¹, sol¹ has abnormal neurophysiology phenotype

(Coombe, 1986)

Phenotype Manifest In

Suppressed by

Statement

Reference

mnb¹ has NMJ bouton phenotype, suppressible | partially by Synj^UAS.Tag:HA/Scer\GAL4^nSyb.PS

(Chen et al., 2014)

mnb¹ has NMJ bouton phenotype, suppressible | partially by Scer\GAL4^nSyb.PS/Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)}

(Chen et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Synj^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^nSyb.PS suppresses the increase in bouton size in mnb¹ mutant NMJs, but does not suppress the reduction in bouton number.

Expression of Synj^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^nSyb.PS significantly rescues the excitatory postsynaptic potential (EPSP) amplitude defects seen in response to high frequency stimulation seen in mnb¹ mutant larvae. However these larvae also show a slightly faster rundown than controls after prolonged stimulation. The miniature EPSP (mEPSP) and endocytosis defects are also rescued.

(Chen et al., 2014)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\PLCD1^{PH.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^nSyb.PS suppresses the increase in bouton size in mnb¹ mutant NMJs, but does not suppress the reduction in bouton number.

(Chen et al., 2014)

Complementation and Rescue Data

Rescued by

mnb¹ is rescued by mnb^UAS.Tag:HA/Scer\GAL4^nSyb.PS

(Chen et al., 2014)

Comments

Pre-synaptic expression of mnb^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^nSyb.PS rescues the bouton and miniature excitatory postsynaptic potential (mEPSP) defects seen in mnb¹ mutants. The synaptic vesicle endocytosis defects are also rescued.

(Chen et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Heisenberg.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mnb1

(Degoutin et al., 2013)

mnb¹

(Brown et al., 2024, Wang et al., 2024, Han et al., 2023, Peng et al., 2021, Geng et al., 2016, Shaikh et al., 2016, Chen et al., 2014, Degoutin et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (14)

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