Open Close
General Information
Symbol
Avic\GFPEGFP.UAS.Tag:PH(hPLCD1)
Species
A. victoria
Name
FlyBase ID
FBal0340412
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-PLCΔPH-GFP, UAS-PLCδ-PH-EGFP, UAS-PLCδ1-PH-GFP
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of EGFP tagged with Tag:PH(hPLCD1). Used to monitor the distribution of phosphatidylinositol 4,5-bisphosphate (PIP2) within the cell.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Hsap\PLCD1PH.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4n-syb.PS results in neuromuscular junction (NMJ) overgrowth in third instar larvae, including increased total branch length and satellite boutons. A less severe increase in NMJ length is seen when Hsap\PLCD1PH.Scer\UAS.T:Avic\GFP-EGFP is expressed under the control of either Scer\GAL4elav-C155 or Scer\GAL4D42.

Expression of Hsap\PLCD1PH.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4n-syb.PS results in reduced synaptic vesicle cycling (reduced uptake of FM 1-43 dye) during intense stimulation. Neurotransmitter characteristics (excitatory junctional current (EJC) and miniature EJC) are similar to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Enhanced by
Suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Expression of Hsap\PLCD1PH.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4nSyb.PS suppresses the increase in bouton size in mnb1 mutant NMJs, but does not suppress the reduction in bouton number.

One copy of synj1 suppresses the neuromuscular junction overgrowth seen when Hsap\PLCD1PH.Scer\UAS.T:Avic\GFP-EGFP is expressed under the control of Scer\GAL4n-syb.PS in third instar larvae.

Expression of Hsap\PLCD1PH.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4n-syb.PS does not enhance the neuromuscular junction overgrowth phenotype seen in tweek1/tweek2 third instar larvae.

Expression of Hsap\PLCD1PH.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4n-syb.PS does not enhance the neuromuscular junction overgrowth seen in WASp1/Df(3R)3450 third instar larvae.

Expression of Hsap\PLCD1PH.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4n-syb.PS enhances the overgrown neuromuscular junction phenotype seen in nwk1 third instar larvae.

Expression of WASp1/Df(3R)3450 does not enhance the enlarged neuromuscular junction phenotype seen when Hsap\PLCD1PH.Scer\UAS.T:Avic\GFP-EGFP is expressed under the control of Scer\GAL4n-syb.PS in homozygous nwk1 third instar larvae.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Avic\GFPEGFP.UAS.Tag:PH(hPLCD1)
Hsap\PLCD1PH.Scer\UAS.T:Avic\GFP-EGFP
Name Synonyms
Secondary FlyBase IDs
  • FBal0241654
  • FBal0340337
References (19)