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General Information
Symbol
Dmel\PRL-12
Species
D. melanogaster
Name
FlyBase ID
FBal0349219
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

Two base deletion leads to a frameshift and early translation termination after the addition of six novel amino acids.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A small deletion results in a frame-shift and premature termination codon within PRL-1 (N36*).

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

PRL-12/Df(2L)r10 transheterozygotes show delayed hatching. Adults have apparently normal size and morphology, but occasionally show held-up wings; they display climbing defects and are unable fly. Their posterior dorsocentral bristle neurons show normal neuron cell body size and morphology, and normal axon caliber, but the contralateral branch covers significantly less territory, as compared to controls; the ventral nerve cord is significantly smaller than in controls, particularly in the metathoracic neuropil; except for slightly shorter posterior projections, due to a significantly smaller VNC, the projections of SC mechanosensory neurons are normal.

In PRL-12 homozygous adults, the posterior dorsocentral bristle neurons show loss of synaptic arborizations in the contralateral axon collateral, and the antennal lobes and mushroom bodies are disorganized, with evident axonal or synaptic defects (e.g. ORN target area appears disrupted/less homogenous and the synaptic termination area is smaller despite of a comparable number of Or83c-positive neurons; mushroom body fusion, with midline crossing of some or all mushroom body axons), as compared to heterozygous controls. The third instar larval neuromuscular junction appears normal. At 72h after puparium formation there are significantly fewer satellite growth cones per axon segment, which extend fewer filopodial protrusions, but no reduction of filopodia emanating from the main branch, as compared to controls; these defects are not observed at 47h and 90h after puparium formation.

PRL-12/PRL-16 posterior dorsocentral bristle neurons show a significant decrease in the number of synapses (Brp-positive puncta) in the contralateral branch, but not in the anterior branch, as compared to controls; mechanosensory neurons show a significant decrease in terminal arbor territory, as compared to controls.

In PRL-12/PRL-14 adults, the ventral nerve cord show comparable cell numbers to controls, and the posterior dorsocentral bristle neurons do not show striking differences in synapse ultrastructure, as compared to controls.

Posterior dorsocentral bristle neurons of individuals that are both PRL-12/PRL-14 transheterozygous and express PRL-1UAS.cPa under the control of Scer\GAL4pnr-MD237 do not show significant projection defects (terminal arbor territory or number of synapses/ Syt1-positive puncta) in the contralateral branch, as compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Suppressor of
Phenotype Manifest In
Suppressed by
NOT suppressed by
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PRL-12
Name Synonyms
Secondary FlyBase IDs
    References (1)