FlyBase Allele Report: Dmel\N[Ax-59d]

General Information

Symbol

Dmel\N^Ax-59d

Species

D. melanogaster

Name

FlyBase ID

FBal0012854

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

Also Known As

Ax^59d, Ax^59d5

Key Links

Allele class

antimorphic allele - genetic evidence

gain of function allele

hypomorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Portin, 2000.2.3, Couso and Martinez Arias, 1994)

gain of function allele

(de Celis et al., 1996)

hypomorphic allele - genetic evidence

(Brennan et al., 1997)

Mutagen

X ray

Progenitor genotype

Cytology

Description

Carries a mutation in the 24th EGF like repeat of the extracellular domain of N.

(Brennan et al., 1999)

Point mutation in EGF repeat 24.

(Brennan et al., 1997)

Amino acid replacement: C972S. C972 falls in EGF repeat number 24.

(Couso and Martinez Arias, 1994)

Nucleotide substitution: GT replaced by ATATA.

Mutation replaces the single cysteine at amino acid postition 972 with two residues, tyrosine and serine (C972YS).

Mutant partially sequenced; correlated with single amino acid replacements within six adjacent EGF-homologous elements of the N protein; has Gly at residue 2057 characteristic of Oregon R rather than Ser of Canton S (FBrf0045770).

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

macrochaeta & pronotum

(Couso and Martinez Arias, 1994)

macrochaeta & wing

(Couso and Martinez Arias, 1994)

macrochaeta | somatic clone

(Portin, 2000.2.3, Portin and Rantanen, 2000)

proneural cluster & dorsal mesothoracic disc

(Brennan et al., 1999)

thorax & macrochaeta

(Rabinow and Birchler, 1990)

thorax & microchaeta

(Rabinow and Birchler, 1990)

wing vein

(Rabinow and Birchler, 1990)

wing vein | somatic clone

(Portin, 2000.2.3)

Detailed Description

Statement

Reference

Homozygous clones (in a Minute background) carry few if any sensory macrochaetae, but macrochaetae can develop along the clone border. Wing vein gaps homozygous for N^Ax-59d (marked with ct^unspecified) are significantly longer than the gaps in the veins of the opposite wing.

(Portin and Rantanen, 2000)

Fast growing clones carry few if any sensory macrochaetae, though macrochaetae can develop along clone borders. Wing vein gaps homozygous for N^Ax-59d are significantly longer than the gaps in the veins of the opposite wing. Wing vein gaps extend slightly longer on the dorsal surface than on the ventral surface of the wing.

(Portin, 2000.2.3)

In N^Ax-59d wing discs, those proneural clusters fated to become the scutellar and dorsocentral bristles have fewer cells than wild-type. Those proneural clusters fated to become the anterior postalar and the posterior supralar bristles have a similar number of cells as wild-type.

(Brennan et al., 1999)

N^Ax-59d/Df(1)N-81k1 animals die as pharate adults with an extreme Abruptex phenotype. Heterozygotes appear wild-type.

(Brennan et al., 1997)

Mutants lack bristles in the wing and notum. Wing margins contain fewer bristles than normal, a phenotype also characteristic of wg mutants.

(Couso and Martinez Arias, 1994)

The Abruptex phenotype of heteroallelic combinations with viable Abruptex alleles is reduced at 29^oC as compared to 25^oC.

(de Celis and Garcia-Bellido, 1994)

Mutations of mam, bib and neur in an heterozygous condition had no effect on the expression of N^Ax-59d or N^Ax-59b except when coupled in cis with N^fa-g. The neurogenic mutations suppress the wing venation phenotype of N.

(Portin and Rantanen, 1991)

Adult wing exhibit missing veins and removal of virtually all micro and macrochaetae on the thorax. Wing phenotype can be suppressed by vg and sca mutations.

(Rabinow and Birchler, 1990)

Expression was suppressed by Dl mutations and enhanced by E(spl) and H mutations.

(Siren and Portin, 1989)

Lethal in combination with N^l1N-ts1 at 29^oC, 10% of N^Ax-59d/N^Ax-tsl flies survive and have an abruptex phenotype at 29^oC.

(Shellenbarger and Mohler, 1975)

Developmental studies of Young have shown that N^Ax-59d is a larval lethal (Kelley, Kidd, Deutsch and Young, 1987). N^Ax-59d placed between spl and N^Co (Welshons).

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

N^Ax-59d, wg^S107 has visible | dominant phenotype

(Brennan et al., 1997)

Df(3R)Delta-FX3, N^Ax-59d has visible | dominant phenotype

(Brennan et al., 1997)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

N^Ax-59d has phenotype, non-enhanceable by Su(H)²

(Brennan et al., 1999)

NOT suppressed by

Statement

Reference

N^Ax-59d has phenotype, non-suppressible by Su(H)²

(Brennan et al., 1999)

Other

Statement

Reference

N^Ax-59d, wg^S107 has adult thoracic sensillum phenotype

(Brennan et al., 1997)

Df(3R)Delta-FX3, N^Ax-59d has adult thoracic sensillum phenotype

(Brennan et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of Su(H)² has no effect on the N^Ax-59d proneural cluster phenotypes.

(Brennan et al., 1999)

N^Ax-59d ; wg^S107 double heterozygotes have a reduced number of thoracic bristles compared to wild-type. N^Ax-59d ; Df(3R)Dl-FX3 double heterozygotes have an increased number of thoracic bristles compared to wild-type.

(Brennan et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

N^spl-1

(Brennan et al., 1997)

N^M1

(Brennan et al., 1997)

Fails to complement

N^60g11

(Brennan et al., 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Green.

Comments

Phenotype partially suppressed in N^Ax-59d/+/+. Interacts with some wg alleles.

(Couso and Martinez Arias, 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ax^59d5

(Portin and Rantanen, 1991, Siren and Portin, 1989, Shellenbarger and Mohler, 1975)

Ax^59d

(de Celis and Bray, 2000, Portin, 2000.2.3, Portin and Rantanen, 2000, Brennan et al., 1999, Wesley, 1999, Brennan et al., 1997, de Celis et al., 1996, Couso and Martinez Arias, 1994, de Celis and Garcia-Bellido, 1994, Simpson et al., 1992, Rabinow and Birchler, 1990, Xu et al., 1990)

N^Ax-59d

Name Synonyms

Secondary FlyBase IDs

References (20)

Report Sections

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