FlyBase Allele Report: Dmel\ninaE[7]

General Information

Symbol

Dmel\ninaE⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0013012

Feature type

allele

Associated gene

Dmel\ninaE

Associated Insertion(s)

Carried in Construct

Also Known As

ninaE^P332

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Kumar and Ready, 1995)

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Amino acid replacement: G128R.

(Kumar and Ready, 1995, Kurada and O'Tousa, 1995)

Missense mutation in the third transmembrane domain.

(Steele and O'Tousa, 1990)

normal transcript level; Gly¹²⁸ --> Arg

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:19,887,586..19,887,586 [-]

Nucleotide change:

G19887586M

Amino acid change:

G128R | ninaE-PA

Reported amino acid change:

G128R|FBrf0080181

Reported amino acid change:

ninaE[7]

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Kumar and Ready, 1995, Kurada and O'Tousa, 1995)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior

(Ribeiro et al., 2018)

abnormal neurophysiology | adult stage

(Xiong et al., 2020)

abnormal neurophysiology | recessive

(Scavarda et al., 1983)

abnormal thermotaxis | larval stage | temperature conditional

(Shen et al., 2011)

Phenotype Manifest In

photoreceptor neuron

(Yarfitz et al., 1994)

retina

(Xiong et al., 2020)

rhabdomere R1

(Scavarda et al., 1983)

rhabdomere R2

(Scavarda et al., 1983)

rhabdomere R3

(Scavarda et al., 1983)

rhabdomere R4

(Scavarda et al., 1983)

rhabdomere R5

(Scavarda et al., 1983)

rhabdomere R6

(Scavarda et al., 1983)

Detailed Description

Statement

Reference

Electroretinograms of ninaE⁷ mutants do not show the expected prolonged depolarization afterpotential to blue light stimuli normally observed in controls.

(Xiong et al., 2020)

ninaE⁷ mutant adult males have reduced ability to orient towards and remain close to a female, compared to controls; they also lose the bias seen in controls to extend the wing closest to, rather than furthest from, the female; following of females, wing extensions and copulation are significantly reduced, compared to controls.

(Ribeiro et al., 2018)

Mutant larvae show strong discrimination between 18[o]C and 24[o]C in a binary choice thermotaxis assay (as do wild-type controls), but discrimination between 18[o]C and either 20 or 22[o]C is eliminated in the mutant larvae.

(Shen et al., 2011)

Individuals develop reasonably normal rhabdomeres and none show membrane curtains.

(Kumar and Ready, 1995)

Reduction in the photopigment content and a similar reduction in sensitivity to light. At maximal excitation intensity the calcium signal has a similar wave form to wild type photoreceptors. A partial recovery of sensitivity of the calcium signal to a second light flask is observed.

(Peretz et al., 1994)

Degeneration of photoreceptors but light-dependent ³⁵S-labelled guanosine 5'-3-0-(thio)triphosphate (³⁵S GTPγS) binding is normal.

(Yarfitz et al., 1994)

More rapid rate of R1-6 photoreceptor degeneration than ninaE⁵, but less rapid than ninaE⁸.

(Leonard et al., 1992)

0.08% of wild-type levels of functional rhodopsin.

(Washburn and O'Tousa, 1992)

Retains regular rhabdomeric membranes.

(Steele and O'Tousa, 1990)

Rhabdomeres of young flies are reduced in size and show aberrant packing of microvilli. Rhabdomeres are nearly wild type in adults.

(O'Tousa et al., 1989)

The electroretinogram (ERG) of homozygous flies is abnormal; they display neither inactivation nor afterpotential, and the on-transient component is absent. Heterozygotes have an intermediate phenotype that varies somewhat with age. Homozygotes have almost no rhodopsin, heterozygotes have approximately 37% rhodopsin compared to wild-type levels. The R1-R6 rhabdomeres have normal morphology in homozygotes, although they are sightly reduced in volume.

(Scavarda et al., 1983)

<2% normal RH1 level

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Camta^tes-2, ninaE⁷ has abnormal neurophysiology phenotype

(Han et al., 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

ninaE⁷ is a suppressor | partially of retina | adult stage phenotype of Culd^e01982

(Xu and Wang, 2016)

ninaE⁷ is a suppressor | partially of photoreceptor neuron | adult stage phenotype of Culd^e01982

(Xu and Wang, 2016)

ninaE⁷ is a suppressor | partially of rhabdomere of eye photoreceptor cell | adult stage phenotype of Culd^e01982

(Xu and Wang, 2016)

ninaE⁷ is a suppressor of retina phenotype of Tsp42Ej¹

(Xu et al., 2004)

ninaE⁷ is a suppressor of phenotype of norpA^P24

(Kurada and O'Tousa, 1995)

Other

Statement

Reference

Camta^tes-2, ninaE⁷ has eye photoreceptor cell phenotype

(Han et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The progressive light-dependent retinal degeneration (loss of photoreceptor cells and rhabdomeres) characteristic for Culd^e01982 mutant adults is suppressed by combination with ninaE⁷. All seven rhabdomeres are still present in 15-day-old Culd^e01982;ninaE⁷ flies kept under light-dark cycle, although the rhabdomeres are smaller than in wild-type.

(Xu and Wang, 2016)

The termination responses of Camta^tes-2; ninaE⁷ double mutant flies are as fast as wild-type flies, but the amplitude of the responses is decreased. In Camta^tes-2; ninaE⁷/+ flies, the response amplitude is similar to wild-type, but the termination response is increased compared to Camta^tes-2 single mutant flies.

(Han et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

2103

y¹ w^67c23; ninaE⁷

Notes on Origin

Discoverer

Pak.

Comments

Encodes 0.1% of wild type levels of R1-6 rhodopsin (Johnson and Pak, J. Gen Physiol. 88:651--673 ).

(Leonard et al., 1992)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

G128R

(Bentrop, 1998)

ninaE³³²

(Kurada and O'Tousa, 1995)

ninaE⁷

(Ribeiro et al., 2018, Chen et al., 2017, Wardill et al., 2012, Han et al., 2006)

ninaE^P332

(Xiong et al., 2020, Xu and Wang, 2016, Shieh et al., 2014, Shen et al., 2011, Han et al., 2006, Wang and Montell, 2005, Lee et al., 2004, Xu et al., 2004, Kumar and Ready, 1995, Running Deer et al., 1995, Peretz et al., 1994, Yarfitz et al., 1994, Ozaki et al., 1993, Leonard et al., 1992, Washburn and O'Tousa, 1992, Steele and O'Tousa, 1990, O'Tousa et al., 1989, Scavarda et al., 1983)

Name Synonyms

Secondary FlyBase IDs

References (26)

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