FlyBase Allele Report: Dmel\osk[8]

General Information

Symbol

Dmel\osk⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0013310

Feature type

allele

Associated gene

Dmel\osk

Associated Insertion(s)

Carried in Construct

Also Known As

osk³⁰¹, oskar³⁰¹

Key Links

Genomic Maps

JBrowse

Allele class

heat sensitive loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

heat sensitive loss of function allele

(Ephrussi et al., 1991)

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: P424S. Nucleotide substitution: C1941T.

(Kim-Ha et al., 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:8,936,758..8,936,758 [+]

Nucleotide change:

C8936758T

Reported nucleotide change:

C1941T

Amino acid change:

P424S | osk-PA; P286S | osk-PC

Reported amino acid change:

P424S

(Kim-Ha et al., 1991)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile | maternal effect

(Hay et al., 1990)

lethal | maternal effect | heat sensitive

(Raff et al., 1990)

lethal | maternal effect | recessive | heat sensitive

(Ephrussi et al., 1991, Lehmann and Nusslein-Volhard, 1991)

male sterile | maternal effect

(Hay et al., 1990)

Phenotype Manifest In

Detailed Description

Statement

Reference

Cap cells are organized as a cluster in the ovaries from offspring of osk⁸/osk⁸ mothers, similarly to wild type.

(Panchal et al., 2017)

Somatic gonadal precursors (SGPs) are specified normally, but primordial germ cells are absent in embryos derived from osk^CE4/osk⁸ females. The SGPs compact into spherical 'gonads' even in the absence of the germ cells.

(Sano et al., 2012)

In embryos laid by osk⁸/osk^CE4 mothers, compaction of somatic gonadal precursors in parasegment 10 (part of embryonic gonadal mesoderm) occurs normally, even though these embryos lack germ cells.

(Jenkins et al., 2003)

The gonadal mesoderm coalesces normally in embryos derived from osk⁸/osk^CE4 females, even though the embryos lack germ cells.

(Van Doren et al., 2003)

Primordial germ cells (PGCs) are absent in the lumen of the posterior midgut (PMG).

(Jaglarz and Howard, 1995)

Primordial germ cells transplanted into a pole-cell-less osk⁸ host migrate according to the host's developmental stage.

(Jaglarz and Howard, 1994)

Temperature-sensitive allele.

(Lindsley and Zimm, 1992)

Embryos derived from homozygous females do not contain pole cells.

(Szabad and Nothiger, 1992)

At the restrictive temperature germline and abdomen formation is affected. At the permissive temperature germline defects remain but abdomen develops normally.

(Ephrussi et al., 1991)

Lack posterior body pattern elements and germ cells.

(Kim-Ha et al., 1991)

Temperature sensitivity is restricted to the abdominal phenotype: at the restrictive temperature embryos lack abdomens.

(Lehmann and Nusslein-Volhard, 1991)

Embryos lack pole cells. Large amounts of the protein encoded by vas are expressed in early stages of oogenesis. The protein encoded by vas fails to distribute asymmetrically. Cleavage embryos have uniform distribution of the protein encoded by vas, which disappears by early gastrulation.

(Lasko and Ashburner, 1990)

Hemizygous embryos derived from homozygous females have no polar granules, fail to form pole cells and have deletions of abdominal structures. Posterior localization of vas and CycB transcripts is completely abolished at 29^oC and at 18^oC CycB transcripts alone are localized at the posterior pole.

(Raff et al., 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

osk⁸/osk^CE4 is a non-suppressor | maternal effect of gonad | embryonic stage phenotype of ena^C14-06

(Sano et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos that are derived from osk^CE4/osk⁸ females and are zygotically homozygous for ena^C14-06 show defects or delays in somatic gonadal precursor cluster adhesion at stage 13 and fail to complete gonad compaction, resulting in extended or split gonads at later stages, as occurs in ena^C14-06 single mutant embryos.

(Sano et al., 2012)

The gonadal mesoderm fails to coalesce in foi^20.71 osk^CE4/foi^16.33 embryos that are derived from osk mutant (foi^20.71 osk^CE4/osk⁸) females.

(Van Doren et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Normal localization of osk mRNA.

(Kim-Ha et al., 1991)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

osk³⁰¹

(Panchal et al., 2017, Aram et al., 2016, Stepanik et al., 2016, Sano et al., 2012, Bader et al., 2011, Arama et al., 2007, Mukai et al., 2007, Arama et al., 2006, Beckett and Baylies, 2006, Le Bras and Van Doren, 2006, DeFalco et al., 2003, Jenkins et al., 2003, Kawashima et al., 2003, Li et al., 2003, Van Doren et al., 2003, Styhler et al., 2002, Volpe et al., 2001, Szabad, 1998, Spradling et al., 1997, Gonczy and DiNardo, 1996, Nakamura et al., 1996, Boyle and DiNardo, 1995, Eberhart and Wasserman, 1995, Jaglarz and Howard, 1995, Rittenhouse and Berg, 1995, Jaglarz and Howard, 1994, Warrior, 1994, Fuller, 1993, St. Johnston, 1993, Gonczy et al., 1992, Szabad and Nothiger, 1992, Ephrussi et al., 1991, Kim-Ha et al., 1991, Lehmann and Nusslein-Volhard, 1991, St. Johnston et al., 1991, Amrein et al., 1990, Hay et al., 1990, Lasko and Ashburner, 1990, Raff et al., 1990, Lehmann, 1988, Tearle and Nusslein-Volhard, 1987)

osk⁸

oskar³⁰¹

(Tanentzapf et al., 2000, Miedema et al., 1995)

Name Synonyms

Secondary FlyBase IDs

References (48)

Report Sections

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