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General Information
Symbol
Dmel\enaC14-06
Species
D. melanogaster
Name
FlyBase ID
FBal0282342
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C19160540T

Reported nucleotide change:

C?T

Amino acid change:

Q389term | ena-PA; Q538term | ena-PB; Q389term | ena-PC; Q392term | ena-PD; Q389term | ena-PE; Q685term | ena-PF; Q369term | ena-PG

Reported amino acid change:

Q388term

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide substitution: C?T.

Amino acid replacement: Q388term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Somatic gonadal precursors (SGPs) are specified normally as three clusters and primordial germ cells (PGCs) are associated with all three clusters in homozygous embryos. However, these clusters fail to adhere to each other at stage 13, and the mutant gonads remain elongated or split at stage 16 in the mutant embryos. Some mis-migrating PGCs are also seen outside the gonad from stage 13 onwards.

The number of SGP protrusions appear to be normal in mutant gonads at stage 15-16, extending between the PGCs as in wild type. The changes in SGP cell morphology and cell orientation that normally happen during gonad compaction do not occur in the mutant embryos.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT suppressed by
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Embryos that are derived from oskCE4/osk8 females and are zygotically homozygous for enaC14-06 show defects or delays in somatic gonadal precursor cluster adhesion at stage 13 and fail to complete gonad compaction, resulting in extended or split gonads at later stages, as occurs in enaC14-06 single mutant embryos.

67.8% of gonads do not form correctly in enaC14-06/+, shgg317/+ transheterozygous embryos, compared to 28.8% in enaC14-06/+ and 37.3% in shgg317/+ single heterozygotes.

More than 50% of gonads do not form correctly in enaC14-06/+, shg2/+ transheterozygous embryos.

51.9% of gonads fail to compact properly in enaC14-06/+, zip1/+ transheterozygous embryos, compared to 33.3% in zip1/+ single heterozygotes.

59.8% of gonads fail to compact properly in enaC14-06/+, arm1/+ transheterozygous embryos, compared to 22.1 in arm1/+ single heterozygotes.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Expression of enaScer\UAS.cGa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not rescue the gonad coalescence defects seen in enaC14-06 embryos.

Expression of enaScer\UAS.cGa under the control of Scer\GAL4how-24B partially rescues the gonad coalescence defects seen in enaC14-06 embryos.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (1)