FlyBase Allele Report: Dmel\sd[12]

General Information

Symbol

Dmel\sd¹²

Species

D. melanogaster

Name

FlyBase ID

FBal0015358

Feature type

allele

Associated gene

Dmel\sd

Associated Insertion(s)

Carried in Construct

Also Known As

sd^47M

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Katzen and Bishop, 1996, Katzen, 1990)

Progenitor genotype

Cytology

Description

157bp deletion removing the majority of intron 8 and 22 nucleotides of exon 9 of sd isoform E21. This removes 7 amino acids and the splice site at the intron 8/exon 9 boundary, possibly resulting in a frameshifted protein. The protein encoded by the sd E7 isoform appears to be unaffected in this mutant.

(Srivastava et al., 2004)

100bp deletion within the sd coding region between +12 and +12.4kb.

(Campbell et al., 1992)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:15,823,484..15,823,640 [+]

Comment:

sd¹² is a 157bp deletion, which removes most of intron 8 as well as 22bp of exon 9.

(Srivastava et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

interommatidial bristle | ectopic | somatic clone

(Garg et al., 2007)

interommatidial bristle | somatic clone

(Garg et al., 2007)

leg | somatic clone

(Garg et al., 2007)

unguis | somatic clone

(Garg et al., 2007)

wing (with sd^ETX4)

(Srivastava et al., 2004)

wing disc | somatic clone

(Liu et al., 2000)

wing margin (with sd^ETX4)

(Srivastava et al., 2004)

Detailed Description

Statement

Reference

sd¹² mutant eye disc clones grow normally, comprising ~17% of the tissue.

(Koontz et al., 2013)

Homozygous clones in the third larval instar eye disc do not show obvious growth defects compared to control clones.

(Wu et al., 2008)

Homozygous clones in the eye are very small and result in eye bristle defects; some of the bristles are shorter than normal and duplications and mispatterning of the bristles is seen.

Homozygous clones in the leg lead to leg truncations.

(Garg et al., 2007)

Homozygotes die as first instar larvae. sd¹²/sd^ETX4 flies have a scalloped wing phenotype, with the loss of most of the wing blade tissue and erosion of the wing margin.

(Srivastava et al., 2004)

Homozygous mutant clones grow normally in the notal and hinge regions of the wing imaginal disc, but not within the wing blade. 2 Days after clone induction only very small clones can sometimes be found in the wing blade. 4 days after induction no mutant cells survive.

(Liu et al., 2000)

Larvae exhibit slow growth. Heterozygotes with viable sd alleles exhibit an extreme wing phenotype.

(Campbell et al., 1991)

larval lethal

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

sd¹²/sd¹² is a suppressor | somatic clone of abnormal neuroanatomy | adult stage phenotype of Df(2R)BSC429/BEAF-32^AB-KO

(Jukam et al., 2016)

sd[+]/sd¹² is a suppressor of abnormal size phenotype of Scer\GAL4^GMR.PF, yki^UAS.cHa

(Wu et al., 2008)

sd[+]/sd¹² is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, yki^UAS.cHa

(Wu et al., 2008)

Other

Statement

Reference

Df(2R)BSC429/BEAF-32^AB-KO, sd¹² has abnormal neuroanatomy | somatic clone | adult stage phenotype

(Jukam et al., 2016)

Phenotype Manifest In

Suppressor of

Statement

Reference

sd¹²/sd¹² is a suppressor | somatic clone of photoreceptor cell R8 of pale ommatidium phenotype of Df(2R)BSC429/BEAF-32^AB-KO

(Jukam et al., 2016)

sd¹²/sd¹² is a suppressor | somatic clone of photoreceptor cell R8 of yellow ommatidium phenotype of Df(2R)BSC429/BEAF-32^AB-KO

(Jukam et al., 2016)

sd¹² is a suppressor of eye disc | somatic clone phenotype of yki^B5

(Koontz et al., 2013)

sd¹² is a suppressor of eye disc | somatic clone phenotype of Tgi^UAS.B

(Koontz et al., 2013)

sd¹²/sd¹² is a suppressor of eye disc | somatic clone phenotype of Scer\GAL4^Tub.PU, yki^UAS.cHa

(Wu et al., 2008)

sd[+]/sd¹² is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, yki^UAS.cHa

(Wu et al., 2008)

Other

Statement

Reference

Df(2R)BSC429/BEAF-32^AB-KO, sd¹² has photoreceptor cell R8 of pale ommatidium | somatic clone phenotype

(Jukam et al., 2016)

Df(2R)BSC429/BEAF-32^AB-KO, sd¹² has photoreceptor cell R8 of yellow ommatidium | somatic clone phenotype

(Jukam et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

sd¹²/sd¹² retinal clones have suppression of the increased proportion of pale R8 photoreceptors in flies with BEAF-32^AB-KO/Df(2R)BSC429, to the extent that it is reduced compared to wild-type controls.

(Jukam et al., 2016)

sd¹² suppresses the reduction in clone growth seen in yki^B5 mutant eye discs. The clones comprise ~15% of the eye disc.

sd¹² suppresses the reduction in clone size seen in the eye discs of larvae expressing Tgi^Scer\UAS.B.

(Koontz et al., 2013)

The large eye phenotype caused by expression of yki^Scer\UAS.cHa under the control of Scer\GAL4^GMR.PF is suppressed by sd¹²/+.

The large size and round shape of clones in the eye disc expressing yki^Scer\UAS.cHa under the control of Scer\GAL4^tub.PU is suppressed if the clones are also homozygous for sd¹².

(Wu et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

sd^ETX4

(Srivastava et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (5)

Bloomington

9371

y¹ w^* sd¹² P{neoFRT}18A/FM7a

78368

sd¹² P{neoFRT}19A/FM6; P{neoFRT}42D P{Ubi-mRFP.nls}2R

78369

sd¹² P{neoFRT}19A/FM6; P{neoFRT}82B P{Ubi-mRFP.nls}3R

78370

sd¹² P{neoFRT}19A/FM6; P{Ubi-mRFP.nls}2L P{neoFRT}40A/CyO

78371

sd¹² P{neoFRT}19A/FM6; P{GawB}mirr^DE/TM6B, Tb¹

Notes on Origin

Discoverer

Katzen.

Comments

"157bp deletion removing the majority of intron 8 and 22 nucleotides" was stated as revision. "of exon 9 of sd isoform E21. This removes 7 amino acids and the" was stated as revision. "splice site at the intron 8/exon 9 boundary, possibly resulting in" was stated as revision. "a frameshifted protein. The protein encoded by the sd E7 isoform" was stated as revision. "appears to be unaffected in this mutant." was stated as revision.

(Srivastava et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Sd^47M

(Mitchell et al., 2024)

em47

(Katzen and Bishop, 1996)

sd¹²

sd^47M

(Tokamov et al., 2021, Guo et al., 2019, Manning et al., 2018, Xu et al., 2018, Yu and Pan, 2018, Jukam et al., 2016, Liu et al., 2016, Ferguson and Martinez-Agosto, 2014, Jukam et al., 2013, Jukam et al., 2013, Koontz et al., 2013, Wu et al., 2008, Garg et al., 2007, Srivastava et al., 2004, Liu et al., 2000, Campbell et al., 1992, Campbell et al., 1991)

sd^47m

(Meserve and Duronio, 2015, Halder et al., 1998)

sd^EM47

(Katzen, 1990)

Name Synonyms

Secondary FlyBase IDs

References (22)

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