tub2/tubR5.6 females produce embryos that are severely dorsalised.
The ability of homozygous larvae to encapsulate L.boulardi eggs is significantly reduced compared to that of control larvae.
The concentration of circulating hemocytes in homozygous larvae is reduced compared to controls.
Pupal lethal when heterozygous with the MBT chromosome.
Hemocyte density in the hemolymph of homozygous larvae is significantly lower than in the hemolymph of control larvae.
Level of Drs induction of bacterially challenged tub2/tub1 mutants is lower than in wild type. Pattern of response of CecA1 and CecA2 parallels that of Drs. Dpt and Dro remain fully inducible and pattern of expression of AttA and Def in intermediate. Infection of tub2/tub3 mutants with A.fumigatus causes 3% survival 3 days postinfection, infection with E.coli causes 71% survival 3 days postinfection.
Strongly dorsalised embryos. Transheterozygotes with tub1 are completely dorsalised, the phenotype of tub2 Tl3t/tub1 is unchanged from the transheterozygote. Transheterozygotes with tub5 are weakly dorsalised, the phenotype of tub2 Tl3t/tub5 is lateralised. Transheterozygotes with tub4 are moderately dorsalised, the phenotype of tub2 Tl3t/tub1 is unchanged from the transheterozygote.
Injected dl- perivitelline fluid had no effect on embryos.
tub1/tub2 heterozygous embryos gastrulate symmetrically and secrete a twisted and hollow tube of dorsal cuticle.
Dorsalized embryos: all cuticle cells along the dorsoventral axis behave like dorsal cells of the wild type embryo. zen expression pattern refines at stage 5, dpp pattern does not refine at all, twi and sna are expressed during late stages of embryogenesis.
Embryos derived from homozygous females are dorsalised.