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General Information
Symbol
Dmel\tub2
Species
D. melanogaster
Name
FlyBase ID
FBal0017243
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
tub238
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G4388347A

Reported nucleotide change:

G418A

Amino acid change:

E140K | tub-PC

Reported amino acid change:

E140K

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide substitution: G418A. Amino acid replacement: E140K.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

tub2/tubR5.6 females produce embryos that are severely dorsalised.

The ability of homozygous larvae to encapsulate L.boulardi eggs is significantly reduced compared to that of control larvae.

The concentration of circulating hemocytes in homozygous larvae is reduced compared to controls.

Pupal lethal when heterozygous with the MBT chromosome.

Hemocyte density in the hemolymph of homozygous larvae is significantly lower than in the hemolymph of control larvae.

Level of Drs induction of bacterially challenged tub2/tub1 mutants is lower than in wild type. Pattern of response of CecA1 and CecA2 parallels that of Drs. Dpt and Dro remain fully inducible and pattern of expression of AttA and Def in intermediate. Infection of tub2/tub3 mutants with A.fumigatus causes 3% survival 3 days postinfection, infection with E.coli causes 71% survival 3 days postinfection.

Strongly dorsalised embryos. Transheterozygotes with tub1 are completely dorsalised, the phenotype of tub2 Tl3t/tub1 is unchanged from the transheterozygote. Transheterozygotes with tub5 are weakly dorsalised, the phenotype of tub2 Tl3t/tub5 is lateralised. Transheterozygotes with tub4 are moderately dorsalised, the phenotype of tub2 Tl3t/tub1 is unchanged from the transheterozygote.

Injected dl- perivitelline fluid had no effect on embryos.

tub1/tub2 heterozygous embryos gastrulate symmetrically and secrete a twisted and hollow tube of dorsal cuticle.

Dorsalized embryos: all cuticle cells along the dorsoventral axis behave like dorsal cells of the wild type embryo. zen expression pattern refines at stage 5, dpp pattern does not refine at all, twi and sna are expressed during late stages of embryogenesis.

Embryos derived from homozygous females are dorsalised.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The variable dorsalized embryonic phenotype of wekEX14 mutants is enhanced by tub2. While 51% of wekEX14 embryos show an extreme dorsalized phenotype, this penetrance is increased to 96% in wekEX14; tub2/+ embryos.

The increased concentration of circulating hemocytes seen in mxcG43 larvae is suppressed by tub2/tub2, but the numbers of podocytes and lamellocytes are still increased in the double mutants compared to wild type.

The zygotic semi-lethality of cact7 homozygotes is strongly suppressed by tub2.

Double mutant combinations with eaD1 are strongly dorsalizing.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Same mutation was introduced into a cDNA and used in RNA injection experiments, proving that the lesion is responsible for the tub maternal effect phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (37)