FlyBase Allele Report: Dmel\usp[2]

General Information

Symbol

Dmel\usp²

Species

D. melanogaster

Name

FlyBase ID

FBal0017651

Feature type

allele

Associated gene

Dmel\usp

Associated Insertion(s)

Carried in Construct

Also Known As

usp^KA21

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

X ray

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Henrich et al., 1994)

loss of function allele

(Schubiger et al., 2005)

Mutagen

X ray

Progenitor genotype

Caused by aberration

Tp(3;1)KA21

Cytology

Description

Nonsense mutation.

(Henrich et al., 1994)

usp² is a transposition mutation with a breakpoint in the middle of the usp transcription unit, interrupting the region encoding the DNA-binding domain.

(Oro et al., 1992)

Breakpoint maps within the second finger of the putative DNA-binding domain at nucleotide positions 661-663, within codon Cys167.

(Oro et al., 1990)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult gut | somatic clone

(Zhang et al., 2024)

anterior pupal spiracle, with usp^N325A

(Jones et al., 2013)

anterior pupal spiracle, with usp^Q288A.L366A

(Jones et al., 2013)

anterior pupal spiracle, with usp^Q288A

(Jones et al., 2013)

dendrite | somatic clone

(Ou et al., 2008)

dorsal mesothoracic disc & neuron | somatic clone

(Schubiger and Truman, 2000)

embryonic/larval anterior spiracle, with usp^hs.PO

(Hall and Thummel, 1998)

embryonic/larval midgut | embryonic stage, with usp^hs.PO

(Hall and Thummel, 1998)

embryonic/larval salivary gland | embryonic stage, with usp^hs.PO

(Hall and Thummel, 1998)

enteroendocrine cell | P-stage | decreased number | somatic clone

(Zhang et al., 2024)

imaginal disc, with usp^hs.PO

(Hall and Thummel, 1998)

larval dorsal multidendritic neuron ddaC | somatic clone

(Ou et al., 2008)

midgut imaginal island, with usp^hs.PO

(Hall and Thummel, 1998)

puparium cuticle, with usp^N325A

(Jones et al., 2013)

puparium cuticle, with usp^Q288A.L366A

(Jones et al., 2013)

puparium cuticle, with usp^Q288A

(Jones et al., 2013)

Detailed Description

Statement

Reference

usp² mutants die at the first larval molt.

usp^Q288A usp² animals progress normally through to apolysis of the the epidermis away from the cuticle (albeit with a briefly longer 3rd instar feeding stage compared to normal larvae). Subsequently, usp^Q288A usp² animals do not form a normal puparium - it is dorso-ventrally flattened, the partially everted anterior spiracles point directly forward, the cuticle is only faintly sclerotized, the 'puparial' cuticle is transparent rather than brown, and head eversion is incomplete. Most animals stop development during the early pupal stage and eventually die.

usp^N325A usp² animals progress normally through to apolysis of the the epidermis away from the cuticle. Subsequently, usp^N325A usp² animals do not show most of the morphogenetic events of puparium formation: anterior spiracles do not evert to their full extension or proper angle, the cuticle does not become barrel-shaped and instead remains soft and untanned. These mutants then die within a day or two.

usp^Q288A.L366A usp² animals progress normally through to apolysis of the the epidermis away from the cuticle. Subsequently, usp^Q288A.L366A usp² animals do not show most of the morphogenetic events of puparium formation: anterior spiracles do not evert to their full extension or proper angle, the cuticle does not become barrel-shaped and instead remains soft and untanned. These mutants then die within a day or two.

(Jones et al., 2013)

The dendritic arbors of homozygous ddaC dendritic arborisation neuron MARCM clones have a reduced number of branches compared to controls.

(Ou et al., 2008)

Homozygous clones in the margin region of the wing disc show precocious differentiation of neurons. Homozygous clones in the margin region of wing discs cultured in vitro in the absence of 20-hydroxyecdysone undergo neurogenesis and initiate axonal outgrowth, in contrast to wild-type tissue which arrests development under these conditions.

(Schubiger and Truman, 2000)

Expression of usp^hs.PO using a single 30 minute 37^oC heat pulse during the first larval instar stage is sufficient to rescue more than 70% of usp² animals to the third instar stage. These rescued animals fail to wander near the end of the third instar stage, maintain their larval shape, become motionless at the surface of the food, fail to respond to touch stimulus and do not evert their anterior spiracles. After several hours in this abnormal "stationary stage", the animals begin to apolyze from their third instar cuticle, retracting from both the anterior and posterior ends. Apolysis is complete by 24 hours after becoming stationary. A supernumerary cuticle covers the posterior two-thirds of the animal. This cuticle is thick, well infiltrated with tracheae and segmentally ridged along the body. Most of these rescued animals die by 72 hours after the stationary phase. Imaginal discs appear normal in rescued third instar larvae and begin to evert following the stationary phase, but arrest their development at a point normally seen 1 hour after puparium formation. The gastric caecae retract, although this occurs gradually over 24 hours. A slight compaction of the larval midgut is seen, but the larval cells do not die and the adult midgut does not form. The number of midgut imaginal cells does not change significantly in the 24 hours following the stationary phase. Larval salivary gland development is normal until the end of the third instar stage, however destruction of the larval salivary gland does not occur and the gland persists until the animal dies.

(Hall and Thummel, 1998)

Most of the eggs laid by usp² mosaic females mutant in the germline are fertilised.

(Oro et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

usp² has lethal | larval stage phenotype, suppressible by usp::Cten\Usp-1^d-c

(Henrich et al., 2000)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Mutant larvae rescued by one copy of usp::Cten\Usp-1^d-c die at the late third instar stage. Rescued usp² mutants fail to wander off the food and show no signs of prepupal contraction, although the cuticle becomes partially tanned. Two copies of the usp::Cten\Usp-1^d-c insertion allows some mutants to survive to adulthood. Most of the non-surviving males die during the prepupal period before head eversion. A few of the surviving males develop slightly bent and twisted legs.

(Henrich et al., 2000)

Complementation and Rescue Data

Rescued by

usp² is rescued by usp^+tJa

(Jones et al., 2013)

usp² is rescued by usp^UAS.cGa/Scer\GAL4^h-1J3

(Ghbeish and McKeown, 2002)

usp² is rescued by usp^tOa

(Schubiger and Truman, 2000)

usp² is rescued by usp^tOa

(Oro et al., 1990)

Partially rescued by

usp² is partially rescued by usp^Q288A

(Jones et al., 2013)

usp² is partially rescued by usp^N325A

(Jones et al., 2013)

usp² is partially rescued by usp^Q288A.L366A

(Jones et al., 2013)

usp² is partially rescued by Scer\GAL4^dpp.57A/usp^UAS.cGa

(Ghbeish and McKeown, 2002)

usp² is partially rescued by usp^hs.PO

(Hall and Thummel, 1998)

Not rescued by

usp² is not rescued by usp^UAS.cGa/Scer\GAL4^GMR.PF

(Ghbeish and McKeown, 2002)

Comments

usp^+tJa rescues usp² first instar larval lethality to adulthood.

usp^Q288A rescues usp² first instar larval lethality to the prepupal stage: larvae progress normally through to apolysis of the the epidermis away from the cuticle (albeit with a briefly longer 3rd instar feeding stage compared to normal larvae). Subsequently, usp^Q288A usp² animals do not form a normal puparium and most animals stop development during the early pupal stage and eventually die.

usp^N325A rescues usp² first instar larval lethality to the prepupal stage: larvae progress normally through to apolysis of the the epidermis away from the cuticle. Subsequently, usp^N325A usp² animals do not show most of the morphogenetic events of puparium formation and die within a day or two.

usp^Q288A.L366A rescues usp² first instar larval lethality to the prepupal stage: larvae progress normally through to apolysis of the the epidermis away from the cuticle. Subsequently, usp^Q288A.L366A usp² animals do not show most of the morphogenetic events of puparium formation and die within a day or two.

(Jones et al., 2013)

usp^tOa rescues the precocious differentiation of neurons seen in homozygous usp² clones in the margin region of the wing disc.

(Schubiger and Truman, 2000)

Male mutant phenotype can be rescued by Dp(1;3)w^vco but the females cannot be rescued.

(Henrich et al., 1994)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

31414

usp²/FM7a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

KA21

(Perrimon et al., 1985)

l(1)KA21

usp²

(Zhang et al., 2024, Jones et al., 2013, Mou et al., 2012, Chavoshi et al., 2010, Bernardi et al., 2009, Ou et al., 2008, Bernardi et al., 2007, Wahlstrom et al., 2006)

usp^KA21

(Henrich et al., 1994, Oro et al., 1992, Oro et al., 1990)

Name Synonyms

Secondary FlyBase IDs

References (21)

Report Sections

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