FlyBase Allele Report: Dmel\Cdk1[216P]

General Information

Symbol

Dmel\Cdk1^216P

Species

D. melanogaster

Name

FlyBase ID

FBal0030730

Feature type

allele

Associated gene

Dmel\Cdk1

Associated Insertion(s)

P{}Cdk1^216P

Carried in Construct

Also Known As

Dmcdc2^216P

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Sigrist et al., 1995, Stern et al., 1993)

Mutagen

PM hybrid dysgenesis

(Stern et al., 1993)

P-element activity

(Clegg et al., 1993)

Progenitor genotype

Associated Insertion(s)

P{}Cdk1^216P

Cytology

Description

Insertion of P element into the 5' untranslated region of cdc2.

(Stern et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:10,386,210..10,386,210

(Clegg et al., 1993, Stern et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage

(Stern et al., 1993)

abnormal size | larval stage

(Stern et al., 1993)

lethal | recessive

(Stern et al., 1993)

lethal - all die before end of pupal stage | recessive

(Clegg et al., 1993)

some die during pupal stage | recessive

(Clegg et al., 1993)

visible | heat sensitive

(Cutforth and Rubin, 1994)

Phenotype Manifest In

adult abdomen

(Cutforth and Rubin, 1994)

imaginal disc

(Stern et al., 1993)

larva

(Stern et al., 1993)

Detailed Description

Statement

Reference

The cdc2^E1-24/cdc2^216P combination shows temperature sensitivity: at 29^oC flies fail to eclose, while at 25^oC viable adults with abdominal defects eclose. Mutations at Cdc37 dominantly enhance this abdominal phenotype, causing profound derangement of abdominal structures, missing bristles, and fused segments. Mutations at Cdc37 also reduced the temperature at which the inviability of cdc2^E1-24/cdc2^216P takes effect.

(Cutforth and Rubin, 1994)

Die after pupariation at the larval-pupal interface. Larvae take 1-2 days longer than wild type to reach the wandering stage. Imaginal discs are missing or rudimentary and larval brains are reduced in size. Endoreplication and polytenization of larval cells is normal, as are salivary gland size and intensity of DNA staining of their polytene nuclei.

(Stern et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

cdc2[+]/Cdk1^216P is a non-suppressor of phenotype of CycB^+t10

(Ji et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Cdk1^216P/Df(2L)J27 is rescued by Cdk1^+t5.8

(Stern et al., 1993)

Partially rescued by

Cdk1^216P/Df(2L)J27 is partially rescued by Cdk1^P131S

(Sigrist et al., 1995)

Cdk1^216P/Df(2L)J27 is partially rescued by Cdk1^G177E

(Sigrist et al., 1995)

Cdk1^216P/Df(2L)J27 is partially rescued by Cdk1^A171T

(Sigrist et al., 1995)

Cdk1^216P/Df(2L)J27 is partially rescued by Cdk1^G43E

(Sigrist et al., 1995)

Comments

Lethality of heterozygotes with Df(2L)J27 can be partially rescued by expression of cdc2^A171T, cdc2^G43E or cdc2^P131S at 18^oC, levels of rescue drop at higher temperatures.

(Sigrist et al., 1995)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Cdc^P

(Clegg et al., 1993)

Cdk1^216P

Dmcdc2^216P

(Sigrist et al., 1995, Cutforth and Rubin, 1994)

cdc2^216P

cdk1^216P

(Ji et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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