FlyBase Allele Report: Dmel\pnr[D1]

General Information

Symbol

Dmel\pnr^D1

Species

D. melanogaster

Name

FlyBase ID

FBal0032458

Feature type

allele

Associated gene

Dmel\pnr

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

loss of function allele

gain of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Heitzler et al., 1996, Ramain et al., 1993)

gain of function allele

(Heitzler et al., 1996)

loss of function allele

(Calleja et al., 1996)

Mutagen

ethyl methanesulfonate

(Heitzler et al., 1996, Ramain et al., 1993)

Progenitor genotype

Cytology

Description

Point mutation in the first zinc finger domain.

(Heitzler et al., 1996)

Nucleotide substitution: G1000A. This causes a Cys to Tyr substitution within a 23 amino acid stretch in the N-terminal zinc finger.

(Ramain et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:16,039,065..16,039,065 [+]

Nucleotide change:

G16039065A

Reported nucleotide change:

G1000A

Amino acid change:

C169Y | pnr-PA; C117Y | pnr-PB; C169Y | pnr-PC; C117Y | pnr-PD

Reported amino acid change:

C?Y

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement. (Figure 3 and Table 1)

(Ramain et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | dominant | wandering third instar larval stage

(Chatterjee et al., 2019)

lethal - all die during embryonic stage | recessive

(Heitzler et al., 1996, Ramain et al., 1993)

some die during embryonic stage | recessive

(Heitzler et al., 1996, Ramain et al., 1993)

visible | dominant

(Asmar et al., 2008, Cubadda et al., 1997, Heitzler et al., 1996, Ramain et al., 1993)

Phenotype Manifest In

amnioserosa

(Ramain et al., 1993)

anterior dorsocentral bristle

(Ramain et al., 1993)

dorsal longitudinal indirect flight muscle cell (with pnr^MD237)

(Ghazi et al., 2003)

dorsocentral bristle

(Vanolst et al., 2005, Cubadda et al., 1997, Calleja et al., 1996, Heitzler et al., 1996)

dorsocentral bristle | ectopic (with pnr^MD237)

(Ghazi et al., 2003)

dorsocentral bristle | increased number

(Asmar et al., 2008, Biryukova and Heitzler, 2008, Biryukova and Heitzler, 2005, Heitzler et al., 2003)

embryonic/larval crystal cell | increased number | wandering third instar larval stage

(Chatterjee et al., 2019)

embryonic dorsal epidermis

(Heitzler et al., 1996, Ramain et al., 1993)

mesonotum (with pnr^MD237)

(Ghazi et al., 2003)

mesothoracic cleft

(Heitzler et al., 1996)

posterior dorsocentral bristle

(Ramain et al., 1993)

postocellar bristle

(Cubadda et al., 1997)

scutellar bristle

(Cubadda et al., 1997, Heitzler et al., 1996)

scutellar bristle | increased number

(Biryukova and Heitzler, 2008, Biryukova and Heitzler, 2005)

Detailed Description

Statement

Reference

Larval crystal cell counts of pnr^D1/pnr^D1 or pnr^D1/+ mutants are higher than that of controls.

(Chatterjee et al., 2019)

Heterozygotes have extra dorsocentral bristles on the notum.

(Asmar et al., 2008)

pnr^D1/+ flies exhibit extra dorsocentral and scutellar sensory organs. Larvae have an increased number of sensory organ precursors.

(Biryukova and Heitzler, 2008)

pnr^D1/+ flies have extra dorsocentral and scutellar bristles on the thorax.

(Biryukova and Heitzler, 2005)

pnr^D1 heterozygotes exhibit 3.34 dorsocentral bristles per heminotum compared to 2.0 in wild-type controls.

(Vanolst et al., 2005)

pnr^MD237/pnr^D1 mutants show abnormal muscle attachments. The dorsal medial muscle (DLM)s appear as a single fibre. The notum also shows defects, a notal cleft is seen along with extra dorsocentral bristles.

(Ghazi et al., 2003)

Heterozygous flies have an excess of dorsocentral and scutellar bristles, and show a loss of postvertical bristles.

(Cubadda et al., 1997)

In pnr^D1/pnr^MD237 mutants there is an increase in number of the wg-dependent dorsocentral bristles.

(Calleja et al., 1996)

Homozygotes exhibit a dorsal hole. Heterozygotes exhibit a slight thoracic cleft defects and extra dorsocentral and scutellar bristles. Dominant phenotype is suppressed by pnr^VX3. Mitotic clones in the thorax adjacent to the thoracic midline cause a sizeable bilateral cleft involving most of the scutum causing the entire scutellum to disappear.

(Heitzler et al., 1996)

Heterozygotes display extra dorsocentral bristles. Similar phenotype is seen in clones homozygous for pnr^D1. Dominant phenotype enhanced in viable transallelic combinations with pnr^V1.

(Ramain et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

pnr^D1 has visible | dominant phenotype, enhanceable by ush^unspecified

(Cubadda et al., 1997)

Suppressed by

Statement

Reference

pnr^D1 has visible | dominant phenotype, suppressible by Bx^hdp-n60

(Asmar et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

pnr^D1 has dorsocentral bristle | increased number phenotype, enhanceable by HDAC1³²⁶/Rpd3[+]

(Biryukova and Heitzler, 2008)

CtBP[+]/CtBP^rev19, pnr^D1 has dorsocentral bristle | increased number phenotype, enhanceable by HDAC1³²⁶/Rpd3[+]

(Biryukova and Heitzler, 2008)

HDAC1³²⁶/Rpd3[+], pnr^D1 has dorsocentral bristle | increased number phenotype, enhanceable by CtBP[+]/CtBP^rev19

(Biryukova and Heitzler, 2008)

pnr^D1 has dorsocentral bristle | increased number phenotype, enhanceable by Sin3A[+]/Sin3A^dQ4

(Biryukova and Heitzler, 2008)

pnr^D1 has dorsocentral bristle | increased number phenotype, enhanceable by CtBP[+]/CtBP^rev19

(Biryukova and Heitzler, 2008)

pnr^D1 has scutellar bristle | increased number phenotype, enhanceable by CtBP[+]/CtBP^rev19

(Biryukova and Heitzler, 2008)

pnr^D1 has dorsocentral bristle | increased number phenotype, enhanceable by HDAC1^def24/Rpd3[+]

(Biryukova and Heitzler, 2008)

pnr^D1 has dorsocentral bristle | increased number phenotype, enhanceable by HDAC1⁰⁴⁵⁵⁶/Rpd3[+]

(Biryukova and Heitzler, 2008)

pnr^D1 has dorsocentral bristle | increased number phenotype, enhanceable by HDAC1³⁰³/Rpd3[+]

(Biryukova and Heitzler, 2008)

pnr^D1 has dorsocentral bristle | increased number phenotype, enhanceable by osa⁶¹⁶

(Heitzler et al., 2003)

pnr^D1 has dorsocentral bristle phenotype, enhanceable by ush^unspecified

(Cubadda et al., 1997)

pnr^D1 has scutellar bristle phenotype, enhanceable by ush^unspecified

(Cubadda et al., 1997)

pnr^D1 has postocellar bristle phenotype, enhanceable by ush^unspecified

(Cubadda et al., 1997)

NOT Enhanced by

Statement

Reference

pnr^D1 has dorsocentral bristle | increased number phenotype, non-enhanceable by Rpd3[+]/HDAC1³¹³

(Biryukova and Heitzler, 2008)

pnr^D1 has dorsocentral bristle | increased number phenotype, non-enhanceable by Sin3A[+]/Sin3A^HW52

(Biryukova and Heitzler, 2008)

pnr^D1 has dorsocentral bristle | increased number phenotype, non-enhanceable by Sin3A[+]/Sin3A^ex4

(Biryukova and Heitzler, 2008)

pnr^D1 has dorsocentral bristle | increased number phenotype, non-enhanceable by Sin3A[+]/Sin3A^EK14

(Biryukova and Heitzler, 2008)

Suppressed by

Statement

Reference

pnr^D1 has dorsocentral bristle | increased number phenotype, suppressible by Bx^hdp-n60

(Asmar et al., 2008)

pnr^D1 has dorsocentral bristle | increased number phenotype, suppressible by tup^isl-1

(Biryukova and Heitzler, 2005)

pnr^D1 has scutellar bristle | increased number phenotype, suppressible by tup^isl-1

(Biryukova and Heitzler, 2005)

pnr^D1 has dorsocentral bristle phenotype, suppressible by Iswi²

(Vanolst et al., 2005)

pnr^D1 has dorsocentral bristle phenotype, suppressible by tou²

(Vanolst et al., 2005)

Suppressor of

Statement

Reference

pnr^D1 is a suppressor of dorsocentral bristle phenotype of tou²

(Vanolst et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The extra dorsocentral bristle phenotype seen in pnr^D1/+ flies is suppressed by Bx^hdp-n60/Y.

(Asmar et al., 2008)

pnr^D1/CtBP^rev19 flies have ectopic thoracic sensory organs, increased compared to pnr^D1/+. The number of larval sensory organ precursors is also increased.

Rpd3^def24/+ enhances the phenotype of pnr^D1/+, resulting in extra dorsocentral sensory organs.

Rpd3⁰⁴⁵⁵⁶/+ enhances the phenotype of pnr^D1/+, resulting in extra dorsocentral sensory organs.

Flies heterozygous for Rpd3³⁰³ or Rpd3³²⁶ and pnr^D1 enhance the phenotype of pnr^D1/+, resulting in extra sensory organs. Flies heterozygous for Rpd3³¹³ and pnr^D1 do not show enhancement of the pnr^D1/+ phenotype.

pnr^D1, CtBP^rev19, Rpd3³²⁶ triple heterozygous mutant flies show enhancement of the dorsocentral bristle phenotype compared with pnr^D1/CtBP^rev19 and pnr^D1/Rpd3³²⁶.

Sin3A^HW52/+, Sin3A^ex4/+ or Sin3A^EK14/+ do not enhance the phenotype of pnr^D1/+, and no extra bristles are observed.

Sin3A^dQ4/+ is an enhancer of the pnr^D1/+ extra sensory organ phenotype.

(Biryukova and Heitzler, 2008)

The extra bristle phenotype of pnr^D1 flies is suppressed by tup^isl-1.

(Biryukova and Heitzler, 2005)

tou² ; pnr^D1/+ flies exhibit 2.17 dorsocentral bristles per heminotum compared to 3.34 per heminotum in pnr^D1 heterozygotes, indicating that tou² suppresses the phenotype. Iswi²/+ ; pnr^D1/+ flies display on average 2.84 dorsocentral bristles per heminotum, so reducing the number observed in pnr^D1/+ single mutants.

(Vanolst et al., 2005)

The pnr^D1/+ bristle phenotype is suppressed when the flies are carrying three copies of ush⁺ and is enhanced when the flies are carrying only one copy of ush⁺.

(Cubadda et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Class A allele. Allelic series for dominant phenotype, going from greatest to least effect pnr^D4 > pnr^D1 > pnr^D2 = pnr^D3.

(Ramain et al., 1993)

Class 4 pnr allele: dominant allele causing a gain of bristles. Allelic series according to the extent of the dorsal hole: pnr¹ > Df(3R)sbd45 = pnr^VX6 = pnr^VP8 = pnr^D1 > pnr^D3.

(Heitzler et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

pnr^D1

(Chatterjee et al., 2019, Fromental-Ramain et al., 2010, Asmar et al., 2008, Biryukova and Heitzler, 2008, Biryukova and Heitzler, 2005)

pnr^D

(Tomoyasu et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (17)

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