FlyBase Allele Report: Dmel\rdgC[306]

General Information

Symbol

Dmel\rdgC³⁰⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0032562

Feature type

allele

Associated gene

Dmel\rdgC

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Steele and O'Tousa, 1990)

Mutagen

ethyl methanesulfonate

(Kurada and O'Tousa, 1995)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology

(Lee et al., 2004, Vinos et al., 1997)

abnormal thermotaxis

(Kwon et al., 2008)

abnormal visual behavior

(Davidson and Steller, 1998)

increased cell death

(Kiselev et al., 2000)

increased cell death | adult stage

(Kohrs et al., 2021)

increased cell death | conditional

(Lee et al., 2013)

visible | recessive

(Steele and O'Tousa, 1990)

Phenotype Manifest In

Detailed Description

Statement

Reference

The electroretinogram response of light-adapted rdgC³⁰⁶ or ninaE^Δ356;ninaE¹⁷,rdgC³⁰⁶ flies to oscillating light of gradually increasing frequencies is comparable to controls at both lower and higher frequencies. Dark-adapted flies show no significant changes in the onset of the frequency-locked response to oscillating light of either low (10Hz) or high (70Hz) frequency compared to wild-type flies, no defects are observed in light-adapted flies at high frequency either.

(Voolstra et al., 2017)

rdgC³⁰⁶/rdgC³⁰⁶ flies show progressive retinal degeneration upon exposure to constant light.

(Lee et al., 2013)

rdgC³⁰⁶ mutant larvae show a statistically significant increase in the preference for 18[o]C.

(Kwon et al., 2008)

The reduction in electroretinogram (ERG) amplitude seen in flies exposed to constant light is accelerated by rdgC³⁰⁶.

(Lee and Montell, 2004)

Mutant flies show a decrease in the amplitude of the electroretinogram (ERG) compared to wild type.

(Lee et al., 2004)

rdgC³⁰⁶ animals kept in the dark for 6 days show normal ommatidial structure and highly ordered rhabdomeric structure typical of healthy photoreceptors, but animals 6 days after a single 10 minute exposure to blue light show extensive disorganisation of ommatidial structure and morphological changes in photoreceptor cells that are characteristic of apoptosis. Vesiculation and disassembly of the rhabdomeric membrane in the R1-R6 cells, and in some cases encasement of the rhabdomere by phagocytotic cells. The rhabdomere of the UV-sensitive R7 cell is unaffected. In contrast to blue-light stimulated animals, mutants 6 days after 10 min blue immediately followed by 10 min orange light exposures are indistinguishable from unstimulated animals. Photoreceptor cells in mutants stimulated by blue light and kept in the dark for 3 days show slightly smaller rhabdomeres, but little evidence of photoreceptor pathology. In contrast, a full blown picture of apoptosis is evident after 6 days. A 10 minute pulse of orange light 3 days after blue light stimulus followed by three additional days of dark shows complete suppression of apoptosis. Mutants exposed to 10 minutes of blue light followed by 4 days of dark incubation show elimination of the Deep Pseudopupil.

(Kiselev et al., 2000)

Ommatidia, after light-rearing for 8 weeks, show the typical features of apoptosis: cytoplasmic condensation, nuclear chromatin condensation and relatively normal looking mitochondria.

(Davidson and Steller, 1998)

Light-dependent retinal degeneration. ERGs and intracellular recordings demonstrate mutant photoreceptors exhibit a notable decrease in the rate of deactivation relative to control flies. Mutants exhibit a prolonged afterpotential (PDA) defect, this is also rescued by ninaE^Δ356.

(Vinos et al., 1997)

Flies carrying one or two copies of ninaE⁺ exhibit retinal degradation.

(Kurada and O'Tousa, 1995)

Third instar foraging larvae show negative photobehaviour indistinguishable from the wild-type response to light. Third instar larvae show a decrease in negative phototaxis from the onset of wandering culminating in random photobehaviour indistinguishable from the response of wild-type larvae.

(Sawin-McCormack et al., 1995)

Ultrastructural analysis of photoreceptor degeneration demonstrates that the histological and physiological deficits result from degeneration triggered by light.

(Steele and O'Tousa, 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

rdgC³⁰⁶ has increased cell death | conditional phenotype, suppressible by Osi21^die4/Osi21^die4

(Lee et al., 2013)

Suppressor of

Statement

Reference

rdgC³⁰⁶ is a suppressor of abnormal thermotaxis phenotype of norpA^P24

(Kwon et al., 2008)

NOT Suppressor of

Statement

Reference

rdgC³⁰⁶ is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.long, Zzzz\GGGGCC^30.UAS.EGFP

(Jiao et al., 2021)

Other

Statement

Reference

BacA\p35^GMR.PH, rdgC³⁰⁶ has wild-type phenotype

(Davidson and Steller, 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

Gαq¹, rdgC³⁰⁶ has ommatidium phenotype, enhanceable by Arr2¹

(Kiselev et al., 2000)

Suppressed by

Statement

Reference

rdgC³⁰⁶ has retina | conditional phenotype, suppressible by Osi21^die4/Osi21^die4

(Lee et al., 2013)

Gαq¹, rdgC³⁰⁶ has ommatidium phenotype, suppressible by Arr2³

(Kiselev et al., 2000)

rdgC³⁰⁶ has ommatidium phenotype, suppressible by shi¹

(Kiselev et al., 2000)

rdgC³⁰⁶ has phenotype, suppressible by ninaE^Δ356

(Vinos et al., 1997)

rdgC³⁰⁶ has phenotype, suppressible by ninaE^D1

(Kurada and O'Tousa, 1995)

rdgC³⁰⁶ has phenotype, suppressible by ninaE^S95F

(Kurada and O'Tousa, 1995)

NOT Suppressor of

Statement

Reference

rdgC³⁰⁶ is a non-suppressor of eye phenotype of Scer\GAL4^GMR.long, Zzzz\GGGGCC^30.UAS.EGFP

(Jiao et al., 2021)

Other

Statement

Reference

Gαq¹, rdgC³⁰⁶ has ommatidium phenotype

(Kiselev et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Osi21^die4/Osi21^die4 partially suppresses the progressive retinal degeneration seen under constant light exposure in rdgC³⁰⁶/rdgC³⁰⁶ flies.

(Lee et al., 2013)

The ability of norpA³⁶ to select 18[o]C is eliminated in a rdgC³⁰⁶ background.

(Kwon et al., 2008)

The light dependant retinal degeneration seen in Gα49B¹, rdgC³⁰⁶ animals is strongly suppressed by Arr2³. The addition of Gα49B¹ and Arr2¹ to rdgC³⁰⁶ show a rapid degeneration of photoreceptor cells. The addition of rdgC³⁰⁶ to Gα49B¹ flies undergo a dramatic and rapid loss of Deep Pseudopupils (DPPs) between days 4 and 6 of days of light exposure. This phenotype is partially rescued by the addition of Arr2³, and enhanced (the numbers of DPPs start to reduce immediately on exposure to light) by the addition of Arr2¹. rdgC³⁰⁶,shi¹ mutants exposed to 10 minutes of blue light followed by 4 days of dark incubation show a partial rescue of the Deep Pseudopupil phenotype seen in rdgC³⁰⁶ flies alone.

(Kiselev et al., 2000)

Light-induced retinal degeneration is blocked by expression of BacA\p35^GMR.PH : photoreceptor cells and organelles are indistinguishable from wild-type. The 'walking optomotor response' for rdgC³⁰⁶/BacA\p35^GMR.PH flies is normal.

(Davidson and Steller, 1998)

ninaE^Δ356 suppresses the deactivation defect seen in rdgC³⁰⁶ flies.

(Vinos et al., 1997)

Flies carrying ninaE⁺/ninaE^D1 or ninaE⁺/ninaE^D2 do not show retinal degradation.

(Kurada and O'Tousa, 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

rdgC³⁰⁶ is rescued by rdgC^+t7.0

(Kwon et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

No rdgC protein is detected by antibody staining in rdgC³⁰⁶ mutants.

(Steele et al., 1992)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

rdgC306

(Jiao et al., 2021)

rdgC³⁰⁶

(Kohrs et al., 2021, Strauch et al., 2018, Voolstra et al., 2018, Voolstra et al., 2017, Lee et al., 2013, Shen et al., 2011, Kwon et al., 2008, Liu et al., 2008, Wang et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (22)

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