wg17en40cP1/wgl-16 flies show the loss of nearly all pseudotracheae, which are structurally characteristic of the distiproboscis. These flies show no significant loss of taste bristles nor any significant change in mediproboscis morphology.
wg1/wg17en40cP1 wing discs show replacement of wing by a duplication of notum structures, which is also seen in the adult flies.
Proctodeal invagination is almost normal until stages 9-10, but much of the proctodeum (except the anterior-most region including the small intestine) begins to degenerate after the onset of germband shortening, resulting in a very tiny epithelial sac. The anal pads also degenerate.
30% of wgP/wg17en40cP1 flies show development of wing-like structures on the prothoracic segment, ranging from rudimentary to well formed.
A maximum of 5 of the 11 notal bristles (the posterior postalar, and the anterior and posterior dorsocentral and scutellar bristles) is sensitive to non-permissive conditions in wgl-12/wg17en40cP1. Dorsocentral, scutellar and posterior postalar bristles are not rescued by down shift after the late third instar. The sensory bristles of the wing are also temperature sensitive during the late third instar and early prepupal stages: the wing blade is of a normal size but is devoid of marginal structures. In wild type discs, but not in wgl-12/wg17en40cP1 discs, cells near the presumptive wing margin stop DNA replication shortly before pupariation.