FlyBase Allele Report: Dmel\Dfd[hs.PK]

General Information

Symbol

Dmel\Dfd^hs.PK

Species

D. melanogaster

Name

heat shock construct of Kuziora

FlyBase ID

FBal0038716

Feature type

allele

Associated gene

Dmel\Dfd

Associated Insertion(s)

Carried in Construct

P{hsDfd}

Key Links

Transgenic product class

wild_type

(Kuziora and McGinnis, 1988)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Kuziora and McGinnis, 1988)

Mutagen

in vitro construct

(McGinnis et al., 1998, Zhao et al., 1993, Kuziora and McGinnis, 1988)

Progenitor genotype

Carried in construct

P{hsDfd}

Cytology

Description

Dfd expression is driven by an Hsp70 promoter.

(Kuziora and McGinnis, 1988)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Dfd

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | heat sensitive

(Kuziora and McGinnis, 1988)

lethal - all die during embryonic stage

(Pederson et al., 1996)

some die during embryonic stage

(Pederson et al., 1996)

Phenotype Manifest In

cirri | ectopic | embryonic stage | heat sensitive

(Pederson et al., 1996)

cirri | ectopic | heat sensitive

(Zhu and Kuziora, 1996)

embryonic mesothoracic segment | heat sensitive

(Zhu and Kuziora, 1996)

embryonic metathoracic segment | heat sensitive

(Zhu and Kuziora, 1996)

embryonic prothoracic segment | heat sensitive

(Zhu and Kuziora, 1996)

external sensory organ | ectopic | heat sensitive

(Pederson et al., 1996)

labial disc | heat sensitive

(Pederson et al., 1996)

labial segment | heat sensitive

(Lin and McGinnis, 1992)

larval labial organ | heat sensitive

(Pederson et al., 1996)

maxillary palp

(Zhao et al., 1993)

mesothoracic segment | heat sensitive

(Lin and McGinnis, 1992)

metathoracic segment | heat sensitive

(Lin and McGinnis, 1992)

mouth hook | ectopic | embryonic stage | heat sensitive

(Pederson et al., 1996)

mouth hook | ectopic | heat sensitive

(Zhu and Kuziora, 1996)

prothoracic segment | heat sensitive

(Lin and McGinnis, 1992)

Detailed Description

Statement

Reference

Heat induced expression causes no abnormalities in antennal or leg structures.

(Kuzin et al., 1997)

Ectopic maxillary structures, cirri and mouth hooks, are detected in the labial and first thoracic segments after appropriate heat shock induction of P{hsDfd}. Head involution is also blocked, the ectopic external sensory organs appearing on the labial lobes are indistinguishable from those seen in other head involution-defective embryos.

(Pederson et al., 1996)

Transforms thoracic segments toward a maxillary identity, as determined by the development of ectopic cirri and mouthooks.

(Zhu and Kuziora, 1996)

Coexpression with abd-A^hs.PS suggests phenotypic suppression of Dfd by abd-A is dependent on gene dosage.

(Sanchez-Herrero et al., 1994)

Dfd is required but not sufficient for 1.28 expression.

(Mahaffey et al., 1993)

Ectopic Dfd is sufficient to activate ectopic expression of the Dll 3' ventral-lateral maxillary enhancer in other segments, and induces maxillary cirri in labial and thoracic segments. Dll mutations do not completely ameliorate this phenotype.

(O'Hara et al., 1993)

Maxillary palps are split into two segments.

(Zhao et al., 1993)

Antibody staining reveals severe defects in es organs in thoracic segment 1 through to abdominal segment 7 and severe defects in dch3 organs in thoracic segment 2. Thoracic segment 3 was partially transformed to lch3. lch5 in abdominal segments 1 to 7 was abnormal.

(Heuer and Kaufman, 1992)

Heat induced expression transforms parts of more posterior segments to maxillary identities, development of maxillary cirri and mouth hooks in labial and thoracic segments. Transformations do not occur in a Dfd mutant background.

(Lin and McGinnis, 1992)

Severe morphological transformations: defective head involution, partial transformation of many head and thoracic segments to a maxillary segment identity. Penetrance of the maxillary transformation phenotype is highest at cellular blastoderm and decreases at later stages and requires an endogenous copy of Dfd.

(Kuziora and McGinnis, 1988)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Dfd^hs.PK, ss^aSc has abnormal size | adult stage phenotype

(Kuzin et al., 1997)

Dfd^hs.PK, ss^aSc has visible phenotype

(Kuzin et al., 1997)

Phenotype Manifest In

Suppressor of

Statement

Reference

Dfd^hs.PK is a suppressor of embryonic epidermis phenotype of Abd-B^M8, Antp^Ns-rvC3, Scr^C1, Ubx^MX2, abd-A^M1

(Macias and Morata, 1996)

Other

Statement

Reference

Dfd^hs.PK, cnc^B.hs has mouth hook | ectopic phenotype

(McGinnis et al., 1998)

Dfd^hs.PK, cnc^B.hs has cirri | ectopic phenotype

(McGinnis et al., 1998)

Dfd^hs.PK, cnc^A.hs has mouth hook | ectopic phenotype

(McGinnis et al., 1998)

Dfd^hs.PK, cnc^A.hs has cirri | ectopic phenotype

(McGinnis et al., 1998)

Dfd^hs.PK, ss^aSc has tarsal segment phenotype

(Kuzin et al., 1997)

Dfd^hs.PK, ss^aSc has third segment of antenna phenotype

(Kuzin et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Heat shocked embryos that are heterozygous for both Dfd^hs.PK and cnc^A.hs develop ectopic maxillary cirri and mouth hooks in 69% of the first thoracic segments. Heat shocked embryos that are heterozygous for both Dfd^hs.PK and cnc^B.hs develop ectopic cirri in only 25% of the first thoracic segments.

(McGinnis et al., 1998)

Induction of expression in ss^aSc flies causes overgrowth of the third antennal segment and of proximal parts of the homeo tarsus. Legs exhibit enlargement of the proximal part of the tarsus.

(Kuzin et al., 1997)

Scr^C1 Antp^Ns-rvC3 Ubx^MX2 abd-A^M1 Abd-B^M8 larvae (deficient for activity of thoracic and abdominal homeotic genes) exhibit sclerotic plates anterior to each denticle belt. Expression of Dfd^hs.PK suppresses the differentiation of the sclerotic plates.

(Macias and Morata, 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

3228

P{hsDfd}T58; ry^*

Notes on Origin

Discoverer

McGinnis.

(Zhao et al., 1993)

Kuziora and McGinnis.

(Zeng et al., 1994)

Comments

Heat induced ectopic expression of Dfd activates endogenous Dfd.

(Dessain et al., 1992)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Dfd^hs.PK

Name Synonyms

heat shock construct of Kuziora

Secondary FlyBase IDs

References (25)

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