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General Information
Symbol
Dmel\AntpNs-rvC3
Species
D. melanogaster
Name
FlyBase ID
FBal0000605
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
AntpNS+RC3, AntpNs+C3
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology

Polytene chromosomes normal.

Nature of the lesion
Statement
Reference

All Antp promoter activity is lost.

Lesion removes the Antp coding region.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Heterozygotes have a fully penetrant transformation of antenna towards leg.

The number of LinA-derived neurons is reduced compared to wild type in AntpNs-rvC3 mutant clones examined at the late third instar larval stage. This phenotype is seen in thoracic segments T1 and T2, but not in T3.

Homozygous clones in the femur show transformation to third antennal segment tissue. Homozygous clones in more proximal and distal parts of the leg produce well differentiated leg tissue, although the morphology of the leg is disturbed (segment fusions are seen).

Homozygous clones in the legs of segments T1 or T3 result in fusions between the femur and tibia in about 20% of cases. Where fusions are seen, the leg segments are also approximately 50% smaller than normal.

Clones in the leg exhibit some cells that develop into third antennal segment while others develop into normal leg structures.

Lethal when heterozygous with Antp17, Antp1 or Antp23.

Antp is necessary for Scr activation in the posterior compartments of the thorax and abdomen.

Homozygous clones transformed at the blastoderm stage or larval stage transform portions of the second leg into corresponding portions of the antenna. Clones in the wing, mesonotum and first and second leg show abnormal bristle patterns. The phenotype of triply mutant clones AntpNs-rvC3 ScrC1 Ubx1 at the blastoderm stage or larval stage is the additive phenotype of AntpNs-rvC3 and Scr13A Ubx1 clones, portions of all three legs are transformed to antennae, clones in the haltere and metanotum form wing and mesonotum structures and transformation of labial palps of the proboscis into maxillary palps. The phenotype of AntpNs-rvC3 ScrC1 clones and AntpNs-rvC3 Ubx1 clones induced in the head, pro- and mesothorax during embryonic and larval development are indistinguishable from AntpNs-rvC3 ScrC1 Ubx1 clones.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

AntpNs-rvC3 has visible | dominant phenotype, suppressible | partially by MED192/MED19[+]

AntpNs-rvC3 has homeotic | dominant phenotype, suppressible | partially by MED192/MED19[+]

NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Suppressed by
NOT suppressed by
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

MED192/+ partially suppresses the antenna-to-leg transformation seen in AntpNs-rvC3/+ flies, such that the double heterozygotes have antennal aristae.

The number of LinA-derived neurons is dramatically reduced compared to wild type in ScrC1 AntpNs-rvC3 UbxMX12 triple mutant clones examined at the late third instar larval stage. This phenotype is seen in all three thoracic segments. The number of glia derived from the triple mutant LinA neuroblast clones is also reduced compared to wild type.

Adult motor neurons derived from ScrC1 AntpNs-rvC3 UbxMX12 triple mutant LinA neuroblast clones target the same region of the leg as do wild-type LinA-derived neurons. However, fewer branches are observed compared with wild-type clones, with the distal region of the tibia being most highly affected.

Single cell class IV dendrite arborisation (da) neuron clones that are triply mutant for ScrC1, AntpNs-rvC3, UbxMX12 show severe defects in dendrite growth, branching and coverage in segment T3 as well as axon fasciculation defects. Analysis of ScrC1, AntpNs-rvC3, UbxMX12 triple mutant ddaC neuron clones indicates that although the major dendrites of the mutant neurons grow to a similar extent as wild-type controls in the interval between 80 and 96 hours after egg laying, the mutant clones have an increased number of terminal dendrites compared to controls. The mutant clones also show increased dynamic behaviour over this time period compared to controls, due to increased dendrite branch initiation/growth. The number of dendrite retractions is not altered in the mutant neurons.

ScrC1 AntpNs-rvC3 double mutant embryos develop ectopic sclerites, which often look like the cuticular scar tissue that often surrounds the healed wound generated by a sterile needle in late-stage wild-type embryos.

ScrC1 AntpNs-rvC3 double mutant stage 17 embryos have failures in epidermal integrity; dye injected into the perivitelline space penetrates the body cavity in the mutant embryos.

AntpNs-rvC3 ssD115.7 double mutant clones in the leg shoe segment fusions, but no transformation of leg to antenna.

Appendages in thoracic segment 2 (T2) which are composed of a AntpNs-rvC3 hthMeis1-P2 double mutant clone are leg-like along their entire proximo-distal (P-D) axis. However they have only two distinct segments along the P-D axis; a complete tarsus (with five subsegments and a claw) and a single proximal segment (which likely results from a fusion of the four proximal-most segments of a wild-type leg). The appendage shows polarity along the P-D axis (as in wild type), with bristles and trichomes usually pointing distally and distinct bristle types being seen at different positions along the P-D axis. In the proximal segment, two to three spurs are usually seen distally (this type of bristle is normally found in the distal tibia). AntpNs-rvC3 hthMeis1-P2 ScrC1 triple mutant clones result in two-segment appendages in T1 and T2.

The transformation of genitalia into leg seen in Abd-BD18 mutant clones is still seen in Abd-BD18 AntpNs-rvC3 double mutants.

AntpNs-rvC3 ScrC1 abd-AM1 Abd-BM8 quadruple mutant embryos secrete cuticle which has a reiteration of the A1 segment throughout the abdomen. If these embryos are also mutant for hthC1 the abdominal segments all resemble the third abdominal segment.

Quintuple ScrC1, AntpNs-rvC3, UbxMX2, abd-AM1, Abd-BM8 homozygous larvae (deficient for activity of thoracic and abdominal homeotic genes) exhibit sclerotic plates (sp) anterior to each denticle belt. Expression of Dfdhs.PK, labhs.PH or Ubxhs.PG suppresses the differentiation of the sp.

Xenogenetic Interactions
Statement
Reference

Expression of BacA\p35Scer\UAS.cHa under the simultaneous control of both Scer\GAL4VGlut-OK371 and Scer\GAL4tub.PU rescues the reduced number of LinA-derived neurons seen in ScrC1 AntpNs-rvC3 UbxMX12 triple mutant clones examined at the late third instar larval stage. The reduction in number of glia derived from the triple mutant LinA neuroblast clones is not rescued.

Expression of BacA\p35Scer\UAS.cHa under the simultaneous control of both Scer\GAL4VGlut-OK371 and Scer\GAL4tub.PU partially rescues the defects seen in adult motor neurons of the leg derived from ScrC1 AntpNs-rvC3 UbxMX12 triple mutant LinA neuroblast clones.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

Struhl.

Revertant.

Comments
Comments

Despite indication otherwise (FBrf0051566), the lesion in AntpNs-rvC3 has not been characterized.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (10)
References (32)