FlyBase Allele Report: Dmel\klar[marb-CD4]

General Information

Symbol

Dmel\klar^marb-CD4

Species

D. melanogaster

Name

FlyBase ID

FBal0039645

Feature type

allele

Associated gene

Dmel\klar

Associated Insertion(s)

Carried in Construct

Also Known As

klar^mCD4, klar^CD4

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Fischer et al., 2004)

loss of function allele

(Fischer-Vize and Mosley, 1994)

Mutagen

ethyl methanesulfonate

(Fischer-Vize and Mosley, 1994)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q2203term.

(Guo et al., 2005)

Amino acid replacement: ?2203term.

(Fischer et al., 2004)

Nucleotide substitution: C6607T.

(Fischer et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:435,300..435,300 [-]

Nucleotide change:

C435300T

Reported nucleotide change:

C6607T

Amino acid change:

Reported amino acid change:

?2203term

(Fischer et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased fecundity | female

(Xie and Fischer, 2008)

female semi-sterile

(Xie and Fischer, 2008)

visible | recessive

(Fischer et al., 2004, Fischer-Vize and Mosley, 1994)

Phenotype Manifest In

cone cell

(Xie and Fischer, 2008)

eye

(Xie and Fischer, 2008, Fischer et al., 2004, Fischer-Vize and Mosley, 1994)

eye (with Df(3L)emc-E12)

(Fischer et al., 2004)

eye photoreceptor cell

(Xie and Fischer, 2008)

interommatidial bristle

(Fischer-Vize and Mosley, 1994)

lipid droplet

(Welte et al., 1998)

nucleus & cone cell & eye disc

(Kracklauer et al., 2007)

nucleus & eye photoreceptor cell & eye disc

(Kracklauer et al., 2007)

photoreceptor cell & nucleus

(Fischer et al., 2004)

photoreceptor cell & nucleus (with Df(3L)emc-E12)

(Fischer et al., 2004)

photoreceptor cell R2

(Fischer-Vize and Mosley, 1994)

photoreceptor cell R3

(Fischer-Vize and Mosley, 1994)

photoreceptor cell R4

(Fischer-Vize and Mosley, 1994)

photoreceptor cell R5

(Fischer-Vize and Mosley, 1994)

photoreceptor cell R8

(Fischer-Vize and Mosley, 1994)

retina

(Fischer-Vize and Mosley, 1994)

rhabdomere

(Fischer et al., 2004, Fischer-Vize and Mosley, 1994)

rhabdomere (with Df(3L)emc-E12)

(Fischer et al., 2004)

Detailed Description

Statement

Reference

Striated muscle in klar^marb-CD4 mutant third instar larvae exhibits aggregation of the myonuclei and aberrant nuclear shape, as well as variable nuclear size. As in wild type, the myonuclei are associated with myofibrillar domain.

(Elhanany-Tamir et al., 2012)

klar^marb-CD4 single mutants do not exhibit nuclear positioning defects in the oocyte/nurse cell complex nor dumping phenotype in double mutant ovaries. In addition klar^marb-CD4 mutant eyes exhibit slight malformation, with some R-cell and cone cell nuclei displaced.

klar^marb-CD4 mutants exhibit reduced fertility compared to wild-type: they lay fewer eggs and fewer of the eggs laid hatch.

(Xie and Fischer, 2008)

Photoreceptor and cone cell nuclei fail to migrate apically and are positioned randomly within the apical-basal axis in klar^marb-CD4 mutant eye discs.

(Kracklauer et al., 2007)

klar^marb-CD4 embryos show normal lipid droplet transport.

(Guo et al., 2005)

Homozygotes have a subtle rough eye phenotype. The photoreceptor cells are present, largely in their normal positions, but the rhabdomeres are malformed. In contrast to wild type, where the photoreceptor cell nuclei are apical, in homozygous eye discs, most of the photoreceptor cell nuclei are basal and the remainder are randomly distributed throughout the apical/basal plane. The eye phenotypes of klar^marb-CD4/Df(3L)emc-E12 animals are qualitatively indistinguishable from those of klar^marb-CD4 homozygotes.

(Fischer et al., 2004)

Homozygotes show defects in lipid droplet transport.

(Welte et al., 1998)

Mild rough eye phenotype, with irregular reduced corneal pseudopupils. The ommatidia have the normal complement of photoreceptor cells but the rhabdomeres are malformed. Photoreceptors and cone cells are properly determined and assembled into the eye disc. The morphology of the photoreceptor cells is unusual, as their nuclei are in unusual positions. Primary pigment cells develop normally, and at least most of the secondary and tertiary pigment cells and bristle precursors assemble normally. Photoreceptor nuclei of R8, R2, R5, R3 and R4 lie basally instead of apically, though thes ephotoreceptors appear to differentiate properly.

(Fischer-Vize and Mosley, 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

klar^marb-CD4 has decreased fecundity | female phenotype, enhanceable by Msp300^ΔKASH

(Xie and Fischer, 2008)

NOT Enhanced by

Statement

Reference

klar^marb-CD4 has female semi-sterile phenotype, non-enhanceable by Msp300^ΔKASH

(Xie and Fischer, 2008)

klar^marb-CD4 has visible phenotype, non-enhanceable by koi⁸⁰

(Kracklauer et al., 2007)

klar^marb-CD4 has viable phenotype, non-enhanceable by koi⁸⁰

(Kracklauer et al., 2007)

NOT suppressed by

Statement

Reference

klar^marb-CD4 has visible phenotype, non-suppressible by koi⁸⁰

(Kracklauer et al., 2007)

klar^marb-CD4 has viable phenotype, non-suppressible by koi⁸⁰

(Kracklauer et al., 2007)

NOT Enhancer of

Statement

Reference

klar^marb-CD4 is a non-enhancer of visible phenotype of koi⁸⁰

(Kracklauer et al., 2007)

klar^marb-CD4 is a non-enhancer of viable phenotype of koi⁸⁰

(Kracklauer et al., 2007)

NOT Suppressor of

Statement

Reference

klar^marb-CD4 is a non-suppressor of visible phenotype of koi⁸⁰

(Kracklauer et al., 2007)

klar^marb-CD4 is a non-suppressor of viable phenotype of koi⁸⁰

(Kracklauer et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

klar^marb-CD4 has larval muscle system | third instar larval stage phenotype, enhanceable by Msp300^ΔKASH

(Elhanany-Tamir et al., 2012)

NOT Enhanced by

Statement

Reference

klar^marb-CD4 has cone cell phenotype, non-enhanceable by Msp300^ΔKASH

(Xie and Fischer, 2008)

klar^marb-CD4 has eye photoreceptor cell phenotype, non-enhanceable by Msp300^ΔKASH

(Xie and Fischer, 2008)

klar^marb-CD4 has eye phenotype, non-enhanceable by Msp300^ΔKASH

(Xie and Fischer, 2008)

klar^marb-CD4 has eye phenotype, non-enhanceable by koi⁸⁰

(Kracklauer et al., 2007)

klar^marb-CD4 has nucleus & eye photoreceptor cell & eye disc phenotype, non-enhanceable by koi⁸⁰

(Kracklauer et al., 2007)

klar^marb-CD4 has interommatidial bristle phenotype, non-enhanceable by koi⁸⁰

(Kracklauer et al., 2007)

NOT suppressed by

Statement

Reference

klar^marb-CD4 has eye phenotype, non-suppressible by koi⁸⁰

(Kracklauer et al., 2007)

klar^marb-CD4 has nucleus & eye photoreceptor cell & eye disc phenotype, non-suppressible by koi⁸⁰

(Kracklauer et al., 2007)

klar^marb-CD4 has interommatidial bristle phenotype, non-suppressible by koi⁸⁰

(Kracklauer et al., 2007)

NOT Enhancer of

Statement

Reference

klar^marb-CD4 is a non-enhancer of eye phenotype of koi⁸⁰

(Kracklauer et al., 2007)

klar^marb-CD4 is a non-enhancer of nucleus & eye photoreceptor cell & eye disc phenotype of koi⁸⁰

(Kracklauer et al., 2007)

klar^marb-CD4 is a non-enhancer of interommatidial bristle phenotype of koi⁸⁰

(Kracklauer et al., 2007)

NOT Suppressor of

Statement

Reference

klar^marb-CD4 is a non-suppressor of eye phenotype of koi⁸⁰

(Kracklauer et al., 2007)

klar^marb-CD4 is a non-suppressor of nucleus & eye photoreceptor cell & eye disc phenotype of koi⁸⁰

(Kracklauer et al., 2007)

klar^marb-CD4 is a non-suppressor of interommatidial bristle phenotype of koi⁸⁰

(Kracklauer et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

In Msp-300^ΔKASH klar^marb-CD4 double mutant third instar larvae the myonuclei are aberrantly positioned. The phenotype is more severe than in either of the single mutants.

(Elhanany-Tamir et al., 2012)

Msp-300^ΔKASH; klar^marb-CD4 double mutants exhibit wild-type nuclear positioning in the oocyte/nurse cell complex, and there is no 'dumpless' phenotype in double mutant ovaries. The double mutant eye phenotype is like klar^marb-CD4 alone; double mutant eyes are not more severely malformed than klar^marb-CD4 eyes, and R-cell and cone cell nuclei are misplaced similarly in both.

The egg-laying defect of klar^marb-CD4 mutants is enhanced in females heterozygous for Msp-300^ΔKASH, and females homozygous for both klar^marb-CD4 and Msp-300^ΔKASH lay only a small fraction of the wild-type number of eggs. The fraction of the eggs from double mutant mothers that hatch, however, is similar to the fraction from klar^marb-CD4 mothers.

(Xie and Fischer, 2008)

koi^HRKO80.w, klar^marb-CD4 double mutant homozygotes have a mutant phenotype identical to either single mutant alone: they are viable with rough eyes.

(Kracklauer et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

klar¹

(Welte et al., 1998)

Rescued by

klar^marb-CD4 is rescued by klar^{glrs.6.Tag:MYC}

(Mosley-Bishop et al., 1999)

Partially rescued by

klar^marb-CD4 is partially rescued by klar^α.ro

(Kracklauer et al., 2007)

klar^marb-CD4 is partially rescued by klar^{glrs.6.Tag:MYC}

(Fischer et al., 2004)

klar^marb-CD4 is partially rescued by klar^glrs.Tag:MYC

(Mosley-Bishop et al., 1999)

klar^marb-CD4 is partially rescued by klar^glrl.Tag:MYC

(Mosley-Bishop et al., 1999)

Not rescued by

klar^marb-CD4 is not rescued by klar^α.ro

(Kracklauer et al., 2007)

klar^marb-CD4 is not rescued by klar^{3'ΔS.glrs.6.Tag:MYC}

(Fischer et al., 2004)

klar^marb-CD4 is not rescued by klar^{5'ΔA.glrs.6.Tag:MYC}

(Fischer et al., 2004)

Comments

Most photoreceptor nuclei are positioned appropriately (i.e. apically) in klar^marb-CD4 mutant eye discs that also have a copy of klar^α.ro. However, cone cell nuclei remain positioned randomly - many are basal to the photoreceptor nuclei.

(Kracklauer et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25668

P{UASp-klar.6Xmyc}NA1, w^*; klar^marb-CD4/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

klar^CD4

(Kracklauer et al., 2010, Xie and Fischer, 2008)

klar^cd4

(Elhanany-Tamir et al., 2012)

klar^mCD4

(Elhanany-Tamir et al., 2012, Kracklauer et al., 2007, Guo et al., 2005, Fischer et al., 2004, Mosley-Bishop et al., 1999)

klar^marb-CD4

kls^marb-CD4

marb^CD4

(Welte et al., 1998)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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