FlyBase Allele Report: Dmel\tok[Δ2-41]

General Information

Symbol

Dmel\tok^Δ2-41

Species

D. melanogaster

Name

FlyBase ID

FBal0040040

Feature type

allele

Associated gene

Dmel\tok

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Kellermeyer et al., 2020)

Mutagen

Delta2-3 transposase

(Nguyen et al., 1994)

Progenitor genotype

tok^G3

(Nguyen et al., 1994)

Cytology

Description

300-800bp deletion that contains the proximal half of P{wG3/4}tok^G3 and upstream tok sequences.

(Nguyen et al., 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior crossvein

(Nguyen et al., 1994)

crossvein (with tok^E1)

(Serpe et al., 2005)

embryonic/larval neuromuscular junction

(Serpe and O'Connor, 2006)

embryonic/larval neuromuscular junction (with tok^E1)

(Serpe and O'Connor, 2006)

larval abdominal segmental nerve

(Serpe and O'Connor, 2006)

larval abdominal segmental nerve (with tok^E1)

(Serpe and O'Connor, 2006)

larval intersegmental nerve

(Serpe and O'Connor, 2006)

larval intersegmental nerve (with tok^E1)

(Serpe and O'Connor, 2006)

larval longitudinal connective (with tok^E1)

(Serpe and O'Connor, 2006)

larval longitudinal connective | embryonic stage

(Kellermeyer et al., 2020)

muscle cell of A1-7 ventral longitudinal muscle 1

(Serpe and O'Connor, 2006)

muscle cell of A1-7 ventral longitudinal muscle 1 (with tok^E1)

(Serpe and O'Connor, 2006)

posterior crossvein

(Nguyen et al., 1994)

Detailed Description

Statement

Reference

tok^Δ2-41 homozygous embryos exhibit longitudinal axon defects.

(Kellermeyer et al., 2020)

tok^Δ2-41/tok^E1 embryos have a normal CNS appearance overall. However, the embryos show a phenotype of variable penetrance in which the Fas2-positive longitudinal bundles are wavy and irregular and the outer bundle is discontinuous or missing. These embryos also show a variety of guidance defects, including delay in intersegmental nerve growth and defective terminal arbor morphology, SNa stalling and additional branching and the failure of SNb to reach ventral muscle targets. tok^Δ2-41 homozygotes also show these defects, but at a slightly lower penetrance.

The axon guidance defects of tok^Δ2-41/tok^E1 mutants persist until larval stages when the animals begin to die. Although muscles 6 and 7 of these mutants show relatively normal NMJs, there is abnormal innervation in the cleft between muscles 12 and 13 and the innervation on muscle 12 is entirely absent, reduced or misrouted. tok^Δ2-41 homozygous embryos also show these innervation defects.

(Serpe and O'Connor, 2006)

Rare tok^Δ2-41/tok^E1 escapers exhibit loss of the crossveins in the wing.

(Serpe et al., 2005)

Lethality during the larval and pupal stages. The few homozygous larvae that do pupate are developmentally delayed. These adult escapers exhibit complete or partial deletion of the anterior and posterior crossveins. Two extra copies of dpp cannot suppress but enhances the tok^Δ2-41 lethal phenotype.

(Nguyen et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

tok^Δ2-41/tok[+] is an enhancer of visible | dominant phenotype of sog^EP7

(Araujo et al., 2003)

tok^Δ2-41/tok[+] is an enhancer of visible | dominant phenotype of sog^EP11

(Araujo et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

tok^Δ2-41/tok^E1 has crossvein phenotype, suppressible by sog^YL26/sog^P129D

(Serpe et al., 2005)

tok^Δ2-41/tok^E1 has crossvein phenotype, suppressible | partially by sog[+]/sog^YL26

(Serpe et al., 2005)

NOT suppressed by

Statement

Reference

tok^Δ2-41/tok^E1 has crossvein phenotype, non-suppressible by Scer\GAL4^A9/tld^UAS.cHa

(Serpe et al., 2005)

tok^Δ2-41/tok^E1 has crossvein phenotype, non-suppressible by tld^UAS.cHa/Scer\GAL4^da.G32

(Serpe et al., 2005)

Enhancer of

Statement

Reference

tok^Δ2-41/tok[+] is an enhancer of wing vein phenotype of sog^EP7

(Araujo et al., 2003)

tok^Δ2-41/tok[+] is an enhancer of wing vein phenotype of sog^EP11

(Araujo et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

sog^YL26/+; tok^Δ2-41/tok^E1 escapers exhibit partial posterior crossveins with significantly more crossvein material than tok^Δ2-41/tok^E1 escapers. Further, crossveins are almost completely restored in sog^YL26/sog^P129D; tok^Δ2-41/tok^E1 animals. Expression of tld^Scer\UAS.cHa under the control of Scer\GAL4^da.G32 or Scer\GAL4^A9 fails to rescue the posterior crossvein phenotype of tok^Δ2-41/tok^E1 mutants.