FlyBase Allele Report: Dmel\aop[ACT.GMR]

General Information

Symbol

Dmel\aop^ACT.GMR

Species

D. melanogaster

Name

activated

FlyBase ID

FBal0042849

Feature type

allele

Associated gene

Dmel\aop

Associated Insertion(s)

Carried in Construct

P{GMR-yan^ACT}

Also Known As

GMR-YAN^ACT, yan^ACT

Key Links

Transgenic product class

missense_variant

(Rebay and Rubin, 1995)

polypeptide_post_translational_processing_variant

(Rebay and Rubin, 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Rebay and Rubin, 1995)

polypeptide_post_translational_processing_variant

(Rebay and Rubin, 1995)

Mutagen

in vitro construct

(Rebay and Rubin, 1995)

Progenitor genotype

aop^WT.GMR

(Rebay and Rubin, 1995)

Carried in construct

P{GMR-yan^ACT}

Cytology

Description

GMR regulatory sequences drive expression of aop in which phosphoacceptor residues at the eight consensus sites within the coding region are replaced with a nonphosphorylatable amino acid, alanine.

(Rebay and Rubin, 1995)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

aop

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death

(Kurada and White, 1998)

visible

(Tang et al., 2001, Kurada and White, 1998)

visible | adult stage

(Loya et al., 2009)

visible | heat sensitive

(Behan et al., 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of aop^ACT.GMR results in a reduced glassy eye phenotype.

(Loya et al., 2009)

Flies that express aop^ACT.GMR have smaller eyes than wild type, with on average 5.2, instead of 8, photoreceptors per ommatidium.

(Li and Carthew, 2005)

Expression of aop^ACT.GMR does not abolish the second mitotic wave in the eye disc.

(Baonza et al., 2002)

Flies carrying aop^ACT.GMR have a rough eye phenotype that is more severe at 29^oC than at 25^oC.

(Behan et al., 2002)

Flies carrying aop^ACT.GMR have a rough eye phenotype.

(Tang et al., 2001)

Severely disrupted eyes due to inhibition of differentiation of photoreceptors R1-R8, the cone cells and other accessory cells in the eye.

(Rebay et al., 2000)

Severe reduction in eye size and complete abolition of normal ommatidial lattice. Partial transformation of remaining eye tissue into head cuticle seen by small hairlike structures in the ventral posterior region. Pronounced increase in cell death in the anterior portion of the imaginal eye disc.

(Rebay and Rubin, 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

aop^ACT.GMR has visible phenotype, suppressible by lz^Sprite

(Behan et al., 2002)

aop^ACT.GMR has visible phenotype, suppressible by lilli^XS407

(Tang et al., 2001)

aop^ACT.GMR has visible phenotype, suppressible by lilli^s35

(Tang et al., 2001)

aop^ACT.GMR has visible phenotype, suppressible by lilli^XS575

(Tang et al., 2001)

aop^ACT.GMR has increased cell death phenotype, suppressible by In(3L)W^rvX1/Df(3L)X14

(Kurada and White, 1998)

aop^ACT.GMR has increased cell death phenotype, suppressible by BacA\p35^GMR.PH

(Kurada and White, 1998)

Enhancer of

Statement

Reference

aop^ACT.GMR is an enhancer of visible phenotype of lz^ts1

(Behan et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

aop^ACT.GMR has eye phenotype, enhanceable by Df(2R)exu1/Df(2R)mir-7-Δ1

(Li and Carthew, 2005)

aop^ACT.GMR has photoreceptor neuron phenotype, enhanceable by Df(2R)exu1/Df(2R)mir-7-Δ1

(Li and Carthew, 2005)

aop^ACT.GMR has eye phenotype, enhanceable by eya^G130

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by eya^A188

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by rl^A0648

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by Sos^D120

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by Sos^xB10

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by S^BA651

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by S^U132

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by EY2-5^DW1080

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by EY2-6^AC403

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by spen^AH393

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by spen^BU720

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by EY2-8^xCB204

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by Sin3A^DW1046

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by Sin3A^CY886

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by pnt^AP553

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by pnt^AF397

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by EY3-2^xAZ145

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by EY3-2^xCF301

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by EY3-2^xDF411

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by EY3-3^xBE183

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by EY3-3^xF6

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by EY3-5^xZB970

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by EY3-5^xKR845

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, enhanceable by EY2-8^EP1160

(Rebay et al., 2000)

aop^ACT.GMR has phenotype, enhanceable by Sos^EY2-3

(Chen et al., 1999)

NOT Enhanced by

Statement

Reference

aop^ACT.GMR has phenotype, non-enhanceable by retn^x1N793

(Rebay et al., 2000)

aop^ACT.GMR has phenotype, non-enhanceable by EY2-5^AH425

(Rebay et al., 2000)

aop^ACT.GMR has phenotype, non-enhanceable by EY2-6^AQR618

(Rebay et al., 2000)

aop^ACT.GMR has phenotype, non-enhanceable by EY3-4^x1S805

(Rebay et al., 2000)

aop^ACT.GMR has phenotype, non-enhanceable by EY3-4^xII790

(Rebay et al., 2000)

aop^ACT.GMR has phenotype, non-enhanceable by Taf4^AG456

(Rebay et al., 2000)

aop^ACT.GMR has phenotype, non-enhanceable by Taf4^I53

(Rebay et al., 2000)

aop^ACT.GMR has phenotype, non-enhanceable by retn^B142

(Rebay et al., 2000)

Suppressed by

Statement

Reference

Df(2R)exu1/Df(2R)mir-7-Δ1, aop^ACT.GMR has eye phenotype, suppressible by Scer\GAL4^GMR.PF/mir-7^UAS.DsRed2

(Li and Carthew, 2005)

Df(2R)exu1/Df(2R)mir-7-Δ1, aop^ACT.GMR has photoreceptor neuron phenotype, suppressible by Scer\GAL4^GMR.PF/mir-7^UAS.DsRed2

(Li and Carthew, 2005)

aop^ACT.GMR has eye phenotype, suppressible by lz^Sprite

(Behan et al., 2002)

aop^ACT.GMR has ommatidium phenotype, suppressible by lilli^XS407

(Tang et al., 2001)

aop^ACT.GMR has ommatidium phenotype, suppressible by lilli^XS575

(Tang et al., 2001)

aop^ACT.GMR has ommatidium phenotype, suppressible by lilli^s35

(Tang et al., 2001)

aop^ACT.GMR has eye phenotype, suppressible by lilli^DR999

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, suppressible by lilli^DE884

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, suppressible by Su(Tpl)^DI998

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, suppressible by Su(Tpl)^EZ1217

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, suppressible by gl^ER1085

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, suppressible by gl^DZ1053

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, suppressible by stg^D115

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, suppressible by stg^DD937

(Rebay et al., 2000)

aop^ACT.GMR has eye phenotype, suppressible by BacA\p35^GMR.PH

(Kurada and White, 1998)

NOT suppressed by

Statement

Reference

aop^ACT.GMR has phenotype, non-suppressible by EY2-5^AH425

(Rebay et al., 2000)

aop^ACT.GMR has phenotype, non-suppressible by EY2-6^AQR618

(Rebay et al., 2000)

aop^ACT.GMR has phenotype, non-suppressible by EY3-4^x1S805

(Rebay et al., 2000)

aop^ACT.GMR has phenotype, non-suppressible by EY3-4^xII790

(Rebay et al., 2000)

aop^ACT.GMR has phenotype, non-suppressible by Taf4^AG456

(Rebay et al., 2000)

aop^ACT.GMR has phenotype, non-suppressible by Taf4^I53

(Rebay et al., 2000)

aop^ACT.GMR has phenotype, non-suppressible by retn^B142

(Rebay et al., 2000)

aop^ACT.GMR has phenotype, non-suppressible by retn^x1N793

(Rebay et al., 2000)

Enhancer of

Statement

Reference

aop^ACT.GMR is an enhancer of eye phenotype of lz^ts1

(Behan et al., 2002)

Other

Statement

Reference

aop^ACT.GMR, rl^AL528 has eye phenotype

(Rebay et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of mir-7^{sponge.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^ey.PH enhances the glassy eye phenotype seen in aop^ACT.GMR.

(Loya et al., 2009)

The reduced eye phenotype seen in aop^ACT.GMR flies is enhanced in aop^ACT.GMR Df(2R)mir-7-Δ1/Df(2R)exu1 flies, such that these double mutants have, on average, just 3.7 photoreceptors per ommatidium. Coexpression of the mir-7^{Scer\UAS.T:Disc\RFP-DsRed2} transgene, under the control of Scer\GAL4^GMR.PF, prevents the enhancement caused by Df(2R)mir-7-Δ1/Df(2R)exu1.

(Li and Carthew, 2005)

lz^ts1 ; aop^ACT.GMR flies have a markedly reduced eye at 29^oC, with the loss of all ommatidial structures. The rescue of the lz^r1 mutant eye phenotype by lz^mF4 does not occur if the flies also carry aop^ACT.GMR; most ommatidial structure is lost in these flies.

(Behan et al., 2002)

The increased apoptosis phenotype caused by aop^ACT.GMR is slightly suppressed by BacA\p35^GMR.PH but more completely suppressed by Df(3L)X14/In(3L)W^rvX1 (i.e. where W function is lacking).

(Kurada and White, 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

5786

w^*; P{GMR-yan^ACT}VIa

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

GMR-Yan^ACT

(Chen et al., 1999)

aop^ACT.GMR

yan^ACT

(Cassidy et al., 2019, Rebay and Rubin, 1995)

Name Synonyms

activated

(Rebay and Rubin, 1995)

Secondary FlyBase IDs

References (13)

Report Sections

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