FlyBase Allele Report: Dmel\dpp[UAS.cRa]

General Information

Symbol

Dmel\dpp^UAS.cRa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Ruberte

FlyBase ID

FBal0042989

Feature type

allele

Associated gene

Dmel\dpp

Associated Insertion(s)

Carried in Construct

H{UAS-dpp.R}

Also Known As

UAS-dpp

Key Links

Transgenic product class

wild_type

(Ruberte et al., 1995)

Mutagen

P-element activity

Nature of the Allele

Transgenic product class

wild_type

(Ruberte et al., 1995)

Mutagen

P-element activity

(Ruberte et al., 1995)

Progenitor genotype

dpp^P.UAS

(Ruberte et al., 1995)

Carried in construct

H{UAS-dpp.R}

Cytology

Description

The P-element sequences of the dpp^P.UAS progenitor allele, together with the y^+t7.7 allele that they flank have excised, leaving dpp expression under the control of UAS regulatory sequences.

(Ruberte et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dpp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^hs.2sev

(Muzzopappa and Wappner, 2005)

lethal | heat sensitive, with Scer\GAL4^CY2

(Muzzopappa and Wappner, 2005)

lethal | heat sensitive, with Scer\GAL4^e22c

(Muzzopappa and Wappner, 2005)

lethal | heat sensitive, with Scer\GAL4^hs.PH

(Muzzopappa and Wappner, 2005)

lethal | heat sensitive, with Scer\GAL4^T155

(Muzzopappa and Wappner, 2005)

Phenotype Manifest In

dorsal appendage, with Scer\GAL4^CY2

(Muzzopappa and Wappner, 2005)

dorsal appendage, with Scer\GAL4^e22c

(Muzzopappa and Wappner, 2005)

dorsal appendage, with Scer\GAL4^hs.PH

(Muzzopappa and Wappner, 2005)

dorsal appendage, with Scer\GAL4^T155

(Muzzopappa and Wappner, 2005)

dorsal appendage | ectopic, with Scer\GAL4^hs.PH

(Muzzopappa and Wappner, 2005)

dorsal appendage | ectopic | heat sensitive, with Scer\GAL4^hs.PH

(Muzzopappa and Wappner, 2005)

dorsal appendage | heat sensitive, with Scer\GAL4^hs.PH

(Muzzopappa and Wappner, 2005)

egg operculum, with Scer\GAL4^CY2

(Muzzopappa and Wappner, 2005)

egg operculum, with Scer\GAL4^T155

(Muzzopappa and Wappner, 2005)

embryonic/larval dorsal tracheal branch, with Scer\GAL4^btl.PS

(Steneberg et al., 1999)

embryonic/larval tracheal lateral trunk, with Scer\GAL4^btl.PS

(Chen et al., 1998)

germarium, with Scer\GAL4^e22c

(Muzzopappa and Wappner, 2005)

germarium, with Scer\GAL4^hs.PH

(Muzzopappa and Wappner, 2005)

germline cyst, with Scer\GAL4^e22c

(Muzzopappa and Wappner, 2005)

germline cyst, with Scer\GAL4^hs.PH

(Muzzopappa and Wappner, 2005)

larval tracheal system, with Scer\GAL4^btl.PS

(Vincent et al., 1997)

larval tracheal system, with Scer\GAL4^hs.2sev

(Vincent et al., 1997)

maxillary palp, with Scer\GAL4^dpp.blk1

(Yasunaga et al., 2006)

oocyte, with Scer\GAL4^e22c

(Muzzopappa and Wappner, 2005)

oocyte, with Scer\GAL4^hs.PH

(Muzzopappa and Wappner, 2005)

presumptive embryonic/larval tracheal system, with Scer\GAL4^btl.PS

(Ebner et al., 2002)

pseudotrachea, with Scer\GAL4^dpp.blk1

(Yasunaga et al., 2006)

pseudotrachea | increased number, with Scer\GAL4^dpp.blk1

(Yasunaga et al., 2006)

stalk follicle cell, with Scer\GAL4^e22c

(Muzzopappa and Wappner, 2005)

stalk follicle cell, with Scer\GAL4^hs.PH

(Muzzopappa and Wappner, 2005)

tracheal branch primordium, with Scer\GAL4^hs.2sev

(Ruberte et al., 1995)

Detailed Description

Statement

Reference

Expression of dpp^Scer\UAS.cRa under the control of Scer\GAL4^dpp.blk1 results in temperature-dependent defects in palpi. At 28^oC, pseudotracheal regions are almost completely eliminated and putative dorsal bristles are increased. The morphology of the mediproboscis appears almost normal.

(Yasunaga et al., 2006)

Mothers that express dpp^Scer\UAS.cRa under the control of Scer\GAL4^CY2 produce eggs that in 80% of cases have eggshells with enlarged opercula and reduced dorsal appendages. When dpp^Scer\UAS.cRa is expressed under the control of Scer\GAL4^T155 76% of eggshells have enlarged opercula and reduced or malformed dorsal appendages, although these phenotypes are weaker than those observed with the Scer\GAL4^CY2 driver. Expression of dpp^Scer\UAS.cRa driven by Scer\GAL4^hs.PH, following a heat shock, causes 38% of eggshells to display variable phenotypes, including ventralized or fused dorsal appendages and ectopic dorsal appendage material. When no heat shock is applied 29% of eggshells have ectopic dorsal appendage material deposited at the base and often display short or malformed dorsal appendages. When dpp^Scer\UAS.cRa expression is driven by Scer\GAL4^e22c 17% of eggshells exhibit ramified, fused or expanded dorsal appendages. The ovarioles of females that express dpp^Scer\UAS.cRa under the control of either Scer\GAL4^hs.PH or Scer\GAL4^e22c exhibit abnormal germaria, two complete cysts encapsulated within a single follicular epithelium, mislocalized oocytes and lack of interfollicular stalks between adjacent follicles.

(Muzzopappa and Wappner, 2005)

Tracheal cells fail to migrate anteriorly and instead more cells migrate dorsally in embryos expressing dpp^Scer\UAS.cRa under the control of Scer\GAL4^btl.PS.

(Ebner et al., 2002)

Expression driven by Scer\GAL4^btl.PS in a wild type background produces an extra fusion cell per tracheal branch.

(Steneberg et al., 1999)

When expression is driven by Scer\GAL4^btl.PS a dorsal tracheal migration phenotype is observed with a lack of anterio-posterior branch formation.

(Chen et al., 1998)

Scer\GAL4^hs.2sev- and Scer\GAL4^btl.PS-mediated expression during stage 11 inhibits subsequent development of all anteriorly growing branches. By stage 17 many cells have moved to the dorsal side of the embryo such that dorsal branches (consisting of 5 to 7 cells in wild type embryos) consist of 25 cells.

(Vincent et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^αTub84B.PBb/dpp^UAS.cRa is a suppressor of increased cell death | somatic clone | cell non-autonomous phenotype of Myc^αTub84B.PBb

(Moreno and Basler, 2004)

dpp^UAS.cRa/Scer\GAL4^ey.PH is a suppressor | partially of visible phenotype of upd1^unspecified/Df(1)os1A2

(Bach et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^btl.PS, dpp^UAS.cRa has embryonic/larval dorsal tracheal branch phenotype, enhanceable by N^l1N-ts1

(Steneberg et al., 1999)

Suppressed by

Statement

Reference

Scer\GAL4^CY2, dpp^UAS.cRa has egg operculum phenotype, suppressible by slmb^UAS.cGa/Scer\GAL4^CY2

(Muzzopappa and Wappner, 2005)

Enhancer of

Statement

Reference

dpp^UAS.cRa/Scer\GAL4^btl.PS is an enhancer of embryonic/larval dorsal tracheal branch phenotype of N^l1N-ts1

(Steneberg et al., 1999)

Suppressor of

Statement

Reference

dpp^UAS.cRa/Scer\GAL4^ey.PH is a suppressor | partially of eye phenotype of upd1^unspecified/Df(1)os1A2

(Bach et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

slmb^Scer\UAS.cGa overexpression suppresses the expanded operculum phenotype of eggs laid by females overexpressing dpp^Scer\UAS.cRa under the control of Scer\GAL4^CY2.

(Muzzopappa and Wappner, 2005)

The small eye phenotype of upd1^unspecified/Df(1)os1A2 flies is partly suppressed by expression of dpp^Scer\UAS.cRa under the control of Scer\GAL4^ey.PH.

(Bach et al., 2003)

Expression driven by Scer\GAL4^btl.PS in a N^l1N-ts1 background further increases the number of fusion cells.

(Steneberg et al., 1999)

Defects in tracheal cell migration caused by ectopic dpp expression in early embryonic stages are totally suppressed in put and tkv mutants.

(Ruberte et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Ruberte.

(Grieder et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dpp^Scer\UAS.cRa

dpp^UAS.cRa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Ruberte

(Ruberte et al., 1995)

Secondary FlyBase IDs

References (19)

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