FlyBase Allele Report: Dmel\fz[I.hs]

General Information

Symbol

Dmel\fz^I.hs

Species

D. melanogaster

Name

FlyBase ID

FBal0043088

Feature type

allele

Associated gene

Dmel\fz

Associated Insertion(s)

Carried in Construct

P{hsfzI}

Key Links

Transgenic product class

cDNA

(Krasnow and Adler, 1994)

wild_type

(Krasnow and Adler, 1994)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Krasnow and Adler, 1994)

wild_type

(Krasnow and Adler, 1994)

Mutagen

in vitro construct

(Axelrod et al., 1998, Krasnow and Adler, 1994)

Progenitor genotype

Carried in construct

P{hsfzI}

Cytology

Description

fz cDNA for transcript variant I, expressed from an Hsp70 promoter

(Krasnow and Adler, 1994)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity | dominant | heat sensitive

(Axelrod et al., 1998)

abnormal planar polarity | heat sensitive

(Winter et al., 2001)

visible | heat sensitive

(Winter et al., 2001, Krasnow and Adler, 1994)

wild-type

(Krasnow and Adler, 1994)

Phenotype Manifest In

eye | heat sensitive

(Krasnow and Adler, 1994)

macrochaeta | heat sensitive

(Krasnow and Adler, 1994)

wing & microchaeta | distal

(Adler et al., 1997)

wing | heat sensitive

(Axelrod et al., 1998)

wing hair

(Krasnow et al., 1995)

wing hair | heat sensitive

(Winter et al., 2001, Krasnow and Adler, 1994)

wing hair | increased number | heat sensitive

(Winter et al., 2001)

Detailed Description

Statement

Reference

Expression of fz^I.hs 30 hours after puparium formation results in a multiple wing hair phenotype. Expression of fz^I.hs at 24 hours results in a hair orientation defect.

(Winter et al., 2001)

Overexpression of fz^I.hs at 24 hours after pupariation results in a dominant gain-of-function planar cell polarity phenotype in the wing.

(Axelrod et al., 1998)

Heat induced expression, by waxing the wing, causes a strong tissue polarity phenotype across the wing. Application of hot wax in distal wing regions causes a dramatic hair polarity phenotype, reverse polarity is observed. Little or no effect is seen after waxing of proximal regions.

(Adler et al., 1997)

Heat shock 6 or more hours before prehair initiation results in phenotypes that resemble those of fz, dsh or pk - hsfz-early phenotype. Heat shock just before prehair initiation results in distinct phenotypes that resemble those of fy or in mutants - hsfz-late phenotype. The late phenotype can be distinguished by the dramatic difference in the number of multiple hair cells (MHCs). dsh¹ but not pk¹ can suppress the MHC phenotype. The early phenotype can be suppressed by reduction in dsh⁺ gene dosage and enhanced by an increase.

(Krasnow et al., 1995)

Heat shock inductions between 0 and 36 hours after pupariation resulted in abnormal wing hair polarity and hair number phenotypes similar to those of tissue polarity mutants. Rough eye and weak thoracic bristle phenotypes also result. Heat shock after 36 hours has no effect. Early heat shocks (before 30hours) result in major polarity disruptions and a few multiple hair cells. Late heat shocks (33-36hrs AP) result in numerous multiple hairs as well as abnormal polarity. An active endogenous fz gene is not required for these effects. Clonal analysis reveals a non-cell autonomy of the hair polarity and multiple hair cell phenotype.

(Krasnow and Adler, 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

fz^I.hs has visible | heat sensitive phenotype, enhanceable by zip[+]/zip¹

(Winter et al., 2001)

NOT Enhanced by

Statement

Reference

fz^I.hs has visible | heat sensitive phenotype, non-enhanceable by wg[+]/wg^l-8

(Winter et al., 2001)

Suppressed by

Statement

Reference

fz^I.hs has abnormal planar polarity | heat sensitive phenotype, suppressible by dsh^unspecified

(Winter et al., 2001)

fz^I.hs has visible | heat sensitive phenotype, suppressible by Rho1^72O/Rho1[+]

(Winter et al., 2001)

fz^I.hs has visible | heat sensitive phenotype, suppressible by ck[+]/ck¹³

(Winter et al., 2001)

fz^I.hs has visible | heat sensitive phenotype, suppressible by dsh¹/dsh[+]

(Winter et al., 2001)

fz^I.hs has visible | heat sensitive phenotype, suppressible by rok[+]/Rok²

(Winter et al., 2001)

fz^I.hs has visible | heat sensitive phenotype, suppressible by sqh^AX3/sqh[+]

(Winter et al., 2001)

fz^I.hs has abnormal planar polarity | dominant | heat sensitive phenotype, suppressible by wg^hs.PN

(Axelrod et al., 1998)

NOT suppressed by

Statement

Reference

fz^I.hs has abnormal planar polarity | heat sensitive phenotype, non-suppressible by Rho1^unspecified

(Winter et al., 2001)

fz^I.hs has abnormal planar polarity | heat sensitive phenotype, non-suppressible by ck^unspecified

(Winter et al., 2001)

fz^I.hs has abnormal planar polarity | heat sensitive phenotype, non-suppressible by Rok^unspecified

(Winter et al., 2001)

fz^I.hs has abnormal planar polarity | heat sensitive phenotype, non-suppressible by sqh^unspecified

(Winter et al., 2001)

fz^I.hs has visible | heat sensitive phenotype, non-suppressible by wg[+]/wg^l-8

(Winter et al., 2001)

Suppressor of

Statement

Reference

fz^I.hs is a suppressor of visible | recessive phenotype of ds^33k

(Adler et al., 1998)

fz^I.hs is a suppressor of visible | recessive phenotype of ds^UAO71

(Adler et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

fz^I.hs has wing hair | increased number phenotype, enhanceable by zip[+]/zip¹

(Winter et al., 2001)

NOT Enhanced by

Statement

Reference

fz^I.hs has wing hair | increased number phenotype, non-enhanceable by wg[+]/wg^l-8

(Winter et al., 2001)

Suppressed by

Statement

Reference

fz^I.hs has wing hair | increased number phenotype, suppressible by Rho1^72O/Rho1[+]

(Winter et al., 2001)

fz^I.hs has wing hair | increased number phenotype, suppressible by ck[+]/ck¹³

(Winter et al., 2001)

fz^I.hs has wing hair | increased number phenotype, suppressible by dsh¹/dsh[+]

(Winter et al., 2001)

fz^I.hs has wing hair | increased number phenotype, suppressible by rok[+]/Rok²

(Winter et al., 2001)

fz^I.hs has wing hair | increased number phenotype, suppressible by sqh^AX3/sqh[+]

(Winter et al., 2001)

fz^I.hs has wing hair phenotype, suppressible by dsh^unspecified

(Winter et al., 2001)

fz^I.hs has wing phenotype, suppressible by wg^hs.PN

(Axelrod et al., 1998)

NOT suppressed by

Statement

Reference

fz^I.hs has wing hair | increased number phenotype, non-suppressible by wg[+]/wg^l-8

(Winter et al., 2001)

fz^I.hs has wing hair phenotype, non-suppressible by Rho1^unspecified

(Winter et al., 2001)

fz^I.hs has wing hair phenotype, non-suppressible by ck^unspecified

(Winter et al., 2001)

fz^I.hs has wing hair phenotype, non-suppressible by Rok^unspecified

(Winter et al., 2001)

fz^I.hs has wing hair phenotype, non-suppressible by sqh^unspecified

(Winter et al., 2001)

Suppressor of

Statement

Reference

fz^I.hs is a suppressor of wing phenotype of ds^33k

(Adler et al., 1998)

fz^I.hs is a suppressor of wing hair phenotype of ds^33k

(Adler et al., 1998)

fz^I.hs is a suppressor of wing phenotype of ds^UAO71

(Adler et al., 1998)

fz^I.hs is a suppressor of wing hair phenotype of ds^UAO71

(Adler et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The hair orientation defect resulting from expression of fz^I.hs at 24 hours is suppressed by reducing dsh gene dosage but not that of Rho1, rok, sqh or ck.

(Winter et al., 2001)

The distinctive ds polarity phenotype of ds^33k/ds^UAO71 flies (regions of reversed polarity on the wing) is suppressed by expression of fz^I.hs 24 hours after white prepupa formation using a 1 hour heat shock. The appendage shape abnormalities and wing vein placement abnormalities are not suppressed.

(Adler et al., 1998)

This fz^I.hs dominant gain-of-function planar cell polarity phenotype is suppressed by co-expression of wg^hs.PN.

(Axelrod et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

fz^I.hs rescues fz²⁴

(Krasnow and Adler, 1994)

Comments

Heat shock inductions at 37^oC for 1 hour dramatically rescues the adult mutant phenotype. The best rescue is obtained when flies are raised at 29^oC, though this rescue is not complete. 94% of the polarity and multiple hair cell phenotype is rescued in otherwise null mutants, but rescue is 100% for the hypomophic fz²⁴. Leg and eye phenotypes are also rescued.

(Krasnow and Adler, 1994)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

fz^I.hs

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close