FlyBase Allele Report: Dmel\svp[UAS.cKa]

General Information

Symbol

Dmel\svp^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kramer

FlyBase ID

FBal0044362

Feature type

allele

Associated gene

Dmel\svp

Associated Insertion(s)

Carried in Construct

P{UAS-svp1.K}

Also Known As

UAS-svp1, UAS-svp

Key Links

Transgenic product class

cDNA

(Kramer et al., 1995)

wild_type

(Kramer et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Kramer et al., 1995)

wild_type

(Kramer et al., 1995)

Mutagen

in vitro construct

(Kramer et al., 1995)

Progenitor genotype

Carried in construct

P{UAS-svp1.K}

Cytology

Description

UASt regulatory sequences drive expression of a cDNA that encodes the svp type 1 isoform.

(Kramer et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

svp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile, with Scer\GAL4^Act5C.PI

(Pitman et al., 2002)

fertile, with Scer\GAL4^dpp.blk1

(Pitman et al., 2002)

fertile, with Scer\GAL4^elav.PLu

(Pitman et al., 2002)

fertile, with Scer\GAL4^GMR.PF

(Pitman et al., 2002)

lethal, with Scer\GAL4^sca-T3

(Kramer et al., 1995)

lethal - all die before end of larval stage, with Scer\GAL4^h-1J3

(Pitman et al., 2002)

lethal - all die during embryonic stage, with Scer\GAL4^Dll-md23

(Pitman et al., 2002)

some die during embryonic stage, with Scer\GAL4^Dll-md23

(Pitman et al., 2002)

some die during larval stage, with Scer\GAL4^h-1J3

(Pitman et al., 2002)

viable, with Scer\GAL4^Act5C.PI

(Pitman et al., 2002)

viable, with Scer\GAL4^dpp.blk1

(Pitman et al., 2002)

viable, with Scer\GAL4^elav.PLu

(Pitman et al., 2002)

viable, with Scer\GAL4^GMR.PF

(Pitman et al., 2002)

visible, with Scer\GAL4^GMR.PF

(Pitman et al., 2002)

wild-type, with Scer\GAL4^dpp.blk1

(Pitman et al., 2002)

Phenotype Manifest In

embryo | dorsal closure stage, with Scer\GAL4^pnr-MD237

(Gritzan et al., 2002)

embryonic GW neuron, with Scer\GAL4^en-e16E

(Kanai et al., 2005)

eye, with Scer\GAL4^GMR.PF

(Pitman et al., 2002)

larval EW neuron, with Scer\GAL4^en-e16E

(Kanai et al., 2005)

ommatidium, with Scer\GAL4^GMR.PF

(Pitman et al., 2002)

ommatidium, with Scer\GAL4^sca-537.4

(Chou et al., 1999)

photoreceptor cell R7, with Scer\GAL4^sca-537.4

(Chou et al., 1999)

photoreceptor cell R7, with Scer\GAL4^sca-T3

(Kramer et al., 1995)

photoreceptor cell R8, with Scer\GAL4^sca-537.4

(Chou et al., 1999)

U neuron, with Scer\GAL4^en-e16E

(Kanai et al., 2005)

Detailed Description

Statement

Reference

Larvae expressing svp^Scer\UAS.cKa under the control of Scer\GAL4^so-7.GAL4 show no change in the overall number of photoreceptors in the Bolwig's organ.

(Sprecher et al., 2007)

Early born cells in the NB7-1 and NB7-3 lineages are missing from 30-40% of abdominal hemisegments in svp^Scer\UAS.cKa; Scer\GAL4^en-e16E embryos at stage 15. In 16% of hemisegments in these embryos, the entire NB 7-3 lineage is missing. In contrast, normal numbers of cells in both of these lineages develop in svp^Scer\UAS.cKa; Scer\GAL4^elav-C155 embryos.

(Kanai et al., 2005)

Expression of svp^Scer\UAS.cKa under the control of Scer\GAL4^pnr-MD237 results in dorsal closure defects.

(Gritzan et al., 2002)

When svp^Scer\UAS.cKa is driven by Scer\GAL4^Act5C.PI, Scer\GAL4^elav.PLu, or Scer\GAL4^Alk.6.5 adults are viable and fertile. When driven by Scer\GAL4^dpp.blk1, adults are also viable and fertile with no detectable defects. When svp^Scer\UAS.cKa is driven by Scer\GAL4^Dll-md23 animals die as embryos. When driven by Scer\GAL4^h-1J3 animals die as first instar larvae. When svp^Scer\UAS.cKa is driven by Scer\GAL4^GMR.PF, animals are viable and fertile but have rough eyes, with moderate ommatidial disorganisation.

(Pitman et al., 2002)

All of the ommatidia in flies expressing svp^Scer\UAS.cKa under the control of Scer\GAL4^sca-537.4 lack R7 and R8 photoreceptor cells.

(Chou et al., 1999)

svp^Scer\UAS.cKa/Scer\GAL4^sca-T3 individuals die as embryos or larvae. Adult escapers show mild roughening of the eye due to lack of photoreceptor R7.

(Kramer et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^sca-537.4, svp^UAS.cKa has photoreceptor cell R7 phenotype, suppressible by Ras85D^V12.sev, Scer\GAL4^sca-537.4

(Chou et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The formation of R7 cells is rescued in flies expressing svp^Scer\UAS.cKa under the control of Scer\GAL4^sca-537.4 by expression of Ras85D^V12.sev.

(Chou et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

svp^Scer\UAS.cKa

svp^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kramer

(Kramer et al., 1995)

Secondary FlyBase IDs

References (16)

Report Sections

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