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General Information
Symbol
Dmel\wgAct5C.PS
Species
D. melanogaster
Name
Actin 5C promoter construct of Struhl
FlyBase ID
FBal0044464
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Act5C>wg
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

FRT-y+t7.7 cassette has been excised, juxtaposing Act5C promoter with wg coding sequences.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of wgAct5C.PS in clones during the mid-second larval instar results in a moderate frequency of leg-to-wing transdetermination.

Expression of wgAct5C.PS in the early third larval instar results in a regeneration blastema being formed in the proximodistal portion of the leg disc within 48 hours of induction of wgAct5C.PS expression. This phenotype is seen in 75% of leg discs analysed. The leg disc shows tremendous overgrowth.

Induction of wgAct5C.PS 72 hours after egg deposition results in transdetermination (wing structures in the adult legs) in 25% of cases.

Mutants with flip-out induced wgAct5C.PS clones fail to eclose as viable adults. Some mutants are able to survive to the pharate adult stage; these flies have labial palps that are large and disorganized compared to wild type. Additionally, the palps with wgAct5C.PS clones have twice the wild-type number of pseudotracheal rows and sometimes contain leg cells, as evidenced by the presence of sex comb teeth.

Leg discs that ubiquitously express wgAct5C.PS in the early third larval instar show transdetermination to wing structures, with ventral wing structures (such as yellow club or wing hinge) being seen at dorsal leg positions in 92% of adults. Leg discs that ubiquitously express wgAct5C.PS in the early third larval instar show changes in cell proliferation patterns compared to wild-type leg discs; the discs expressing wgAct5C.PS have a normal pattern of cell division for 1 day after expression is induced, but 1.5 to 2 days after expression is induced, cell proliferation is only maintained in the dorsal regions of the leg disc (in wild-type leg discs, cells divide asynchronously throughout the disc). Portions of leg disc that have been induced to ubiquitously express wgAct5C.PS in the early third larval instar and then transplanted into larval hosts develop into different structures depending on which portion of the leg disc is transplanted. Dorsal halves of leg disc differentiate into ventral wing structures (transdetermination) and also into regenerated structures of the leg. Ventral halves of leg disc differentiate only structures of the ventral leg fate map.

Clones expressing wgAct5C.PS induce scars of pigment rim tissue in the retina; in wild-type eyes such tissue is found only at the extreme periphery of the eye. When these scars are formed in the dorsal region of the eye, dorsal rim-specific ommatidia are found immediately adjacent to the scar. Such ommatidia are normally found adjacent to the the pigment rim tissue at the periphery in the dorsal half of the eye.

Clones in the male external genitalia expressing wgAct5C.PS result in pattern duplications, with the cells expressing wgAct5C.PS contributing to only a small portion of the altered pattern. Small clones result in duplication of bristles. Duplication of almost all structures (including genital arch, lateral lobe, posterior lobe, claspers and anal plates) is seen with larger clones. The penis apparatus can show complete duplication. Two types of clone are seen in the vaginal plates; firstly, duplication of the thorn bristle row is seen, and secondly, complete absence of the thorn bristle row as well as the long bristles is seen.

16% mutants exhibit wg-induced transdetermination of legs with wing tissue.

Clones cause duplication of the dorsal vaginal plate associated with loss of the T8 bristle region and the ventral vaginal plates. Clones causes reduction of the male analia and duplication of the penis apparatus.

Ectopic expression in the eye repolarises the ommatidia over a considerable distance. Ectopic expression in developing ommatidia is deleterious so only a scar of mainly pigment cells remains that indicates the position of the clone.

Clones in the head induce partial loss of a lateral ocellus and an expansion of the frons within the medial ocellar region. Clones in the frons or orbital region show no morphological abnormalities.

A few clones of ectopic wg expression in the pharate adult and adult male foreleg cuticle form wing hinge structures contiguous with leg cuticle. Gain of ventral leg structures and loss of dorsal leg structures also accompany transdetermination to wing.

Clones in the adult eye causes scar formation. Scars run in the anterior posterior direction, are associated with bristle loss in the surrounding ommatidia and sections reveal the scar are devoid of ommatidia but appear to contain pigment cells. In addition the dorsal ventral polarity is reversed in the row of ommatidia next to the scar on the side further from the equator. Scars appear to be the result of delayed or inhibited eye development, causing a block to furrow progression.

wg clones which originate in the tibia and femur can cause complete distalization of the duplicated leg, including formation of tarsal segments with claw organs. Clones in ventrolateral positions cause simple outgrowths of leg that consist entirely of mutant cells. The mutant cells do not affect the fate of the wild type cells around them. Clones expressing wg in the lateral region of the leg do not respecify cell fate. Pattern respecification occurs only when the clones are located dorsally, near the endogenous anterior-posterior compartment boundary. Duplicated legs always bifurcate from the dorsal side of the endogenous leg.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

wgAct5C.PS has visible | somatic clone phenotype, suppressible by ken[+]/ken02970

wgAct5C.PS has visible | somatic clone phenotype, suppressible by oho23B[+]/RpS2103575

wgAct5C.PS has visible | somatic clone phenotype, suppressible by CycA[+]/CycA03946

wgAct5C.PS has visible | somatic clone phenotype, suppressible by polo[+]/polo01673

wgAct5C.PS has visible | somatic clone phenotype, suppressible by eEF1α100248/l(2)00248[+]

wgAct5C.PS has visible | somatic clone phenotype, suppressible by CG4638501482a/CG30497[+]

wgAct5C.PS has visible | somatic clone phenotype, suppressible by jing[+]/jing01094

wgAct5C.PS has visible | somatic clone phenotype, suppressible by cg[+]/cg07659

wgAct5C.PS has visible | somatic clone phenotype, suppressible by Nup15401501/Nup154[+]

wgAct5C.PS has visible | somatic clone phenotype, suppressible by Aly[+]/Ref102267

wgAct5C.PS has visible | somatic clone phenotype, suppressible by Kr-h110642/Kr-h1[+]

wgAct5C.PS has visible | somatic clone phenotype, suppressible by Syx1301470/Syx13[+]

Phenotype Manifest In
Suppressed by
Statement
Reference

wgAct5C.PS has leg | somatic clone phenotype, suppressible by polo[+]/polo01673

wgAct5C.PS has wing | ectopic | somatic clone phenotype, suppressible by polo[+]/polo01673

wgAct5C.PS has wing | ectopic | somatic clone phenotype, suppressible by ken[+]/ken02970

wgAct5C.PS has leg | somatic clone phenotype, suppressible by oho23B[+]/RpS2103575

wgAct5C.PS has wing | ectopic | somatic clone phenotype, suppressible by oho23B[+]/RpS2103575

wgAct5C.PS has leg | somatic clone phenotype, suppressible by CycA[+]/CycA03946

wgAct5C.PS has wing | ectopic | somatic clone phenotype, suppressible by CycA[+]/CycA03946

wgAct5C.PS has leg | somatic clone phenotype, suppressible by eEF1α100248/l(2)00248[+]

wgAct5C.PS has wing | ectopic | somatic clone phenotype, suppressible by eEF1α100248/l(2)00248[+]

wgAct5C.PS has leg | somatic clone phenotype, suppressible by CG4638501482a/CG30497[+]

wgAct5C.PS has wing | ectopic | somatic clone phenotype, suppressible by CG4638501482a/CG30497[+]

wgAct5C.PS has leg | somatic clone phenotype, suppressible by jing[+]/jing01094

wgAct5C.PS has wing | ectopic | somatic clone phenotype, suppressible by jing[+]/jing01094

wgAct5C.PS has leg | somatic clone phenotype, suppressible by cg[+]/cg07659

wgAct5C.PS has wing | ectopic | somatic clone phenotype, suppressible by cg[+]/cg07659

wgAct5C.PS has leg | somatic clone phenotype, suppressible by Kr-h110642/Kr-h1[+]

wgAct5C.PS has leg | somatic clone phenotype, suppressible by Nup15401501/Nup154[+]

wgAct5C.PS has wing | ectopic | somatic clone phenotype, suppressible by Nup15401501/Nup154[+]

wgAct5C.PS has leg | somatic clone phenotype, suppressible by Aly[+]/Ref102267

wgAct5C.PS has wing | ectopic | somatic clone phenotype, suppressible by Aly[+]/Ref102267

wgAct5C.PS has wing | ectopic | somatic clone phenotype, suppressible by Kr-h110642/Kr-h1[+]

wgAct5C.PS has leg | somatic clone phenotype, suppressible by Syx1301470/Syx13[+]

wgAct5C.PS has wing | ectopic | somatic clone phenotype, suppressible by Syx1301470/Syx13[+]

wgAct5C.PS has leg | somatic clone phenotype, suppressible by ken[+]/ken02970

Other
Additional Comments
Genetic Interactions
Statement
Reference

The frequency of leg-to-wing transdetermination caused by expression of wgAct5C.PS in clones during the mid-second larval instar is dominantly suppressed by l(3)0162901629, tai01351, Sec61β07214, polo01673, Kr-h110642, Syx1301470, ken02970, oho23B03575, CycA03946, l(2)0024800248, CG3049701482a, jing01094, cg07659, Nup15401501 or Aly02267.

The formation of a regeneration blastema that is seen in the proximodistal portion of the leg disc after induction of wgAct5C.PS expression in the early third larval instar is delayed by rgn1/+; no blastema is seen in these animals 48 hours after induction of wgAct5C.PS expression, but a regeneration blastema is seen 72 hours after induction of wgAct5C.PS expression.

The overgrowth phenotype that is seen in leg discs after induction of wgAct5C.PS expression in the early third larval instar is strongly suppressed if the animals are also carrying either Alr1/+ or Alr2/+. A blastema is still formed at the normal time in these animals, but it is smaller than that seen in animals in which wgAct5C.PS expression has been induced in an otherwise wild-type background.

The formation of a regeneration blastema that is seen in the proximodistal portion of the leg disc after induction of wgAct5C.PS expression in the early third larval instar still occurs at the normal time if the animals are also carrying Mmp1Q112stop/+. However, the non-blastema cells (ventral leg cells) do not undergo cell-cycle arrest in the wing disc carrying Mmp1Q112stop/+, in contrast to non-blastema cells in animals in which wgAct5C.PS expression has been induced in an otherwise wild-type background.

The frequency of adults that show transdetermination (wing structures in the adult legs) after induction of wgAct5C.PS 72 hours after egg deposition is reduced to 7% if the animals are also carrying rgn1/+.

The frequency of adults that show transdetermination (wing structures in the adult legs) after induction of wgAct5C.PS 72 hours after egg deposition is increased to 53% if the animals are also carrying Alr1/+.

The frequency of adults that show transdetermination (wing structures in the adult legs) after induction of wgAct5C.PS 72 hours after egg deposition is increased to 42% if the animals are also carrying Mmp1Q112stop/+.

wgAct5C.PS; Scer\GAL4dpp.blk1/dppScer\UAS.cSa flies die before adulthood, transplanted second leg discs exhibit wg-induced transdetermination, legs with wing tissue. Coxa, trochanter and femur segments are reduced. Ventral wing hinge structures are observed at opposite regions within the mesothoracic leg tissue. Very few wgAct5C.PS; dppd6/dppd8 and wgAct5C.PS; vg83b27/vg83b27 flies exhibit wg-induced transdetermination, legs with wing tissue. wgAct5C.PS; Scer\GAL4dpp.blk1/biScer\UAS.cBa pupae die before differentiating adult cuticle, transplanted leg discs exhibit an extensive area of wing tissue.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
wgAct5C.PS
Name Synonyms
Actin 5C promoter construct of Struhl
Secondary FlyBase IDs
    References (19)