FlyBase Allele Report: Dmel\wit[unspecified]

General Information

Symbol

Dmel\wit^unspecified

Species

D. melanogaster

Name

FlyBase ID

FBal0046066

Feature type

allele

Associated gene

Dmel\wit

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

FlyBase curator comment: this entry is used to capture phenotypic information when the particular allele (or allele combination) used by the author could not be determined but the context of the experiment suggests that the phenotype being described is some kind of loss of function.

(FlyBase Curators, 2025-)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with wit^A12)

(Sulkowski et al., 2014)

lethal - all die before end of P-stage

(Aberle et al., 2002)

lethal - all die before end of P-stage | recessive

(Harrison et al., 1995)

some die during pharate adult stage

(Aberle et al., 2002)

some die during pharate adult stage | recessive

(Harrison et al., 1995)

Phenotype Manifest In

synapse | larval stage

(Aberle et al., 2002)

terminal bouton (with wit^A12)

(Sulkowski et al., 2014)

Detailed Description

Statement

Reference

Packing of inter-ommatidial cells is normal in somatic clones of wit^unspecified homozygous cells in the pupal retina.

(Cordero et al., 2007)

While muscle size remains normal in mutant larvae, all neuromuscular synapses are smaller than wild-type with nearly 100% penetrance. the size of individual synaptic boutons appeared normal but boutons are often spaced further apart from one another. The specificity of the neuromuscular connections was not affected. The size of the sensory neurons appear normal and the dendritic tree of multidendritic neurons is not visibly reduced in mutants. Neuromuscular synapses in mutant stage 16 embryos appear similar to wild-type, those of the first instar larvae have visibly smaller synapses compared to wild-type, as so those of second instar wit mutants.

(Aberle et al., 2002)

Mutants appear wild-type for axon guidance, and all the synapses of all larval motoneurons appear to be established with their proper targets.

(Marques et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

wit^unspecified has lethal phenotype, suppressible by tkv::wit^{tkv-EC.wit-IC.Ubi-p63E}/tkv::wit^{wit-EC.tkv-IC.Ubi-p63E}

(McCabe et al., 2004)

NOT suppressed by

Statement

Reference

wit^unspecified has lethal phenotype, non-suppressible by sax::wit^{wit-EC.sax-IC.Ubi-p63E}/sax::wit^{sax-EC.wit-IC.Ubi-p63E}

(McCabe et al., 2004)

Enhancer of

Statement

Reference

wit^unspecified is an enhancer of partially lethal - majority die phenotype of Neto¹⁰⁹

(Sulkowski et al., 2014)

Suppressor of

Statement

Reference

wit^unspecified/wit[+] is a suppressor of abnormal neuroanatomy | larval stage phenotype of Vps35^EY14200

(Korolchuk et al., 2007)

Other

Statement

Reference

GluRIIA^SP16/Df(2L)cl-h4, wit^unspecified has lethal | larval stage phenotype

(Haghighi et al., 2003)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

wit^unspecified has neuromuscular junction phenotype, non-suppressible by sax::wit^{wit-EC.sax-IC.Ubi-p63E}/sax::wit^{sax-EC.wit-IC.Ubi-p63E}

(McCabe et al., 2004)

Suppressor of

Statement

Reference

wit^unspecified/wit[+] is a suppressor of NMJ bouton phenotype of Vps35^EY14200

(Korolchuk et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The increased bouton number at the neuromuscular junction that is seen in homozygous Vps35^EY14200 larvae is suppressed by wit^unspecified/+.

(Korolchuk et al., 2007)

Co-expression of sax::wit^{wit-EC.sax-IC.Ubi-p63E} and sax::wit^{sax-EC.wit-IC.Ubi-p63E} only poorly rescues neuromuscular junction size in wit^unspecified mutants. The increase in bouton number at the neuromuscular junction seen in larvae expressing Scer\UBP2^Scer\UAS.cDa under the control of Scer\GAL4^elav.PLu in a wild-type background is not seen if they express Scer\UBP2^Scer\UAS.cDa under the control of Scer\GAL4^elav.PLu in a wit^unspecified background.