FlyBase Allele Report: Dmel\spdo[C55]

General Information

Symbol

Dmel\spdo^C55

Species

D. melanogaster

Name

FlyBase ID

FBal0050554

Feature type

allele

Associated gene

Dmel\spdo

Associated Insertion(s)

Carried in Construct

Also Known As

sanpodo^C55

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Dye et al., 1998, Salzberg et al., 1994)

Mutagen

ethyl methanesulfonate

(Salzberg et al., 1994)

Progenitor genotype

Cytology

Description

Amino acid replacement: L504R.

(Skeath, 2005.2.14)

Amino acid replacement: L??.

(O'Connor-Giles and Skeath, 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:30,478,402..30,478,402 [+]

Nucleotide change:

T30478402G

Amino acid change:

L504R | spdo-PA

Reported amino acid change:

L504R

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Skeath, 2005.2.14, O'Connor-Giles and Skeath, 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Umesono et al., 2002)

increased cell number | embryonic stage

(Drake et al., 2025)

lethal (with spdo^3R6)

(Hutterer and Knoblich, 2005)

lethal | recessive

(Salzberg et al., 1994)

visible | somatic clone

(Upadhyay et al., 2013, Tong et al., 2010)

Phenotype Manifest In

abdominal dorsal bipolar neuron dbp | ectopic

(Umesono et al., 2002)

chaeta | somatic clone

(Upadhyay et al., 2013, Tong et al., 2010)

CNS glial cell

(Dye et al., 1998)

dorsal vessel wall cell

(Gajewski et al., 2000)

embryonic abdominal segment

(Drake et al., 2025)

embryonic peripheral nervous system & glial cell

(Dye et al., 1998)

eo neuron | somatic clone

(Roegiers et al., 2005)

larval anterior commissure

(Dye et al., 1998)

larval dorsal multidendritic neuron | ectopic

(Umesono et al., 2002)

larval longitudinal connective

(Dye et al., 1998)

larval posterior commissure

(Dye et al., 1998)

macrochaeta | somatic clone

(Roegiers et al., 2005)

neuron

(Drake et al., 2025)

presumptive embryonic/larval peripheral nervous system

(Dye et al., 1998)

thecogen cell & peripheral nervous system

(Salzberg et al., 1994)

Detailed Description

Statement

Reference

Homozygous clones in the adult thorax result in a significant loss of bristles.

(Upadhyay et al., 2013)

Homozygous clones in the thorax show extensive bristle loss.

(Tong et al., 2010)

Adult thoraces carrying spdo^C55 mutant clones contain large bald patches due to the absence of external sensory structures.

(Roegiers et al., 2005)

The dorsal bipolar dendritic (dbd) and dorsal dendritic arbor (dda) neurons are duplicated in mutant embryos.

(Umesono et al., 2002)

Increased numbers of svp expressing cardioblasts are seen in mutant embryos - up to seven or eight per segment.

(Gajewski et al., 2000)

Shows severe phenotype in trans to Df(3R)tll-g. spdo^C55 mutant embryos show a loss of all glial cells per hemisegement in the peripheral nervous system except one. These embryos also have reduced numbers of central nervous system glia and gaps in longitudinal tracts of the ventral nerve cord. The anterior and posterior commissures appear thicker and less condensed than normal.

(Dye et al., 1998)

Defect in embryonic PNS development: gain of neurons, glial support cells are transformed into neurons.

(Salzberg et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

spdo^H7

(Dye et al., 1998)

spdo^S097002b

(Salzberg et al., 1997)

Rescued by

spdo^C55 is rescued by spdo^UAS.EGFP/Scer\GAL4^sca-DB

(Upadhyay et al., 2013)

spdo^C55 is rescued by spdo^UAS.EGFP/Scer\GAL4^sca-109-68

(Upadhyay et al., 2013)

spdo^C55 is rescued by spdo^{LHAAA.UAS.EGFP}/Scer\GAL4^sca-DB

(Upadhyay et al., 2013)

spdo^C55 is rescued by spdo^{ΔNPAF.LHAAA.UAS.EGFP}/Scer\GAL4^sca-DB

(Upadhyay et al., 2013)

spdo^C55 is rescued by spdo^UAS.EGFP/Scer\GAL4^sca-DB

(Tong et al., 2010)

spdo^C55 is rescued by spdo^{Δ196-255.UAS.EGFP}/Scer\GAL4^sca-DB

(Tong et al., 2010)

spdo^C55 is rescued by spdo^{NPAA.UAS.EGFP}/Scer\GAL4^sca-DB

(Tong et al., 2010)

spdo^C55 is rescued by spdo^{ΔN18.UAS.EGFP}/Scer\GAL4^sca-DB

(Tong et al., 2010)

Not rescued by

spdo^C55 is not rescued by Scer\GAL4^sca-109-68/spdo^{Δ100-125.UAS.EGFP}

(Upadhyay et al., 2013)

spdo^C55 is not rescued by spdo^{Δ424-565.UAS.EGFP}/Scer\GAL4^sca-DB

(Tong et al., 2010)

spdo^C55 is not rescued by spdo^{Δ1-424.UAS.EGFP}/Scer\GAL4^sca-DB

(Tong et al., 2010)

spdo^C55 is not rescued by spdo^{Δ1-180.UAS.EGFP}/Scer\GAL4^sca-DB

(Tong et al., 2010)

spdo^C55 is not rescued by spdo^{mCD8.1-424.UAS.EGFP}/Scer\GAL4^sca-DB

(Tong et al., 2010)

Comments

Expression of spdo^{Scer\UAS.T:Avic\GFP-EGFP} under the control of either Scer\GAL4^sca-DB or Scer\GAL4^sca-109-68 rescues loss of bristle phenotype of spdo^C55 clones in the adult thorax.

Expression of spdo^{LHAAA.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^sca-DB rescues loss of bristle phenotype of spdo^C55 clones in the adult thorax.

Expression of spdo^{Δ100-125.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^sca-109-68 fails to rescue loss of bristle phenotype of spdo^C55 clones in the adult thorax.

Expression of spdo^{ΔNPAF.LHAAA.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^sca-DB rescues the loss of bristle phenotype of spdo^C55 clones in the adult thorax. However, 29.9% of the rescued bristles have supernumerary socket cells.

(Upadhyay et al., 2013)

Expression of spdo^{Scer\UAS.T:Avic\GFP-EGFP}, spdo^{ΔN18.Scer\UAS.T:Avic\GFP-EGFP} or spdo^{Δ196-255.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^sca-DB rescues the wild-type bristle pattern in spdo^C55 clones in the thorax.

Expression of either spdo^{Δ424-565.Scer\UAS.T:Avic\GFP-EGFP}, spdo^{Δ1-424.Scer\UAS.T:Avic\GFP-EGFP}, spdo^{Δ1-180.Scer\UAS.T:Avic\GFP-EGFP}, spdo^{NPAA.Scer\UAS.T:Avic\GFP-EGFP} or spdo^{1-424.Scer\UAS.T:Mmus\Cd8a,T:Avic\GFP-EGFP} under the control of Scer\GAL4^sca-DB fails to rescue the loss of bristles seen in spdo^C55 clones in the thorax.