FlyBase Allele Report: Dmel\NetA[UAS.cHa]

General Information

Symbol

Dmel\NetA^UAS.cHa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Harris

FlyBase ID

FBal0052148

Feature type

allele

Associated gene

Dmel\NetA

Associated Insertion(s)

Carried in Construct

P{UAS-NETA}

Also Known As

UAS-netA

Key Links

Transgenic product class

cDNA

(Harris et al., 1996)

wild_type

(Harris et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Harris et al., 1996)

wild_type

(Harris et al., 1996)

Mutagen

in vitro construct

(Harris et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-NETA}

Cytology

Description

UASt regulatory sequences drive expression of a NetA cDNA.

(Harris et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

NetA

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon, with Scer\GAL4^sca-537.4

(Harris et al., 1996)

lamina, with Scer\GAL4^Act5C.PP

(Gong et al., 1999)

larval transverse nerve, with Scer\GAL4^F63

(Winberg et al., 1998)

larval transverse nerve, with Scer\GAL4^how-24B

(Winberg et al., 1998)

optic nerve, with Scer\GAL4^GMR.PF

(Gong et al., 1999)

photoreceptor cell & axon, with Scer\GAL4^Act5C.PP

(Gong et al., 1999)

photoreceptor cell & axon, with Scer\GAL4^GMR.PF

(Gong et al., 1999)

symmetrical commissure, with Scer\GAL4^sca-537.4

(Harris et al., 1996)

Detailed Description

Statement

Reference

Pan-neural expression of NetA^Scer\UAS.cHa under the control of Scer\GAL4^sca-109-68 has no effect on apoptosis in the thoracic and abdominal segments of the ventral nerve cord.

(Newquist et al., 2013)

When expression is driven by Scer\GAL4^GMR.PF, retinal axons stall in the optic stalk, spread abnormally or criss-cross each other along the D/V axis. Photoreceptor axons also collapse onto each other along the A/P axis. They fail to innervate their target region properly. When expression is driven in clones in the anterior, undifferentiated portion of the eye disc or in the lamina anlage by Scer\GAL4^Act5C.PP there is no effect on retinal fibers. However when clones fall in the differentiating portion of the lamina, mild hypoinnervation and irregular spacing of retinal fibers occurs.

(Gong et al., 1999)

Scer\GAL4^F63-mediated expression elicits inappropriate innervation by the transverse nerve (ectopic innervation of muscles 7 and 6 and occasionally 13) but can rescue the embryonic phenotype of Ts(1Lt;YSt)B118+Ts(1Rt;YLt)T9. Scer\GAL4^how-24B-mediated expression allows the axons of the transverse nerve to ectopically innervate muscles 7 and 6.

(Winberg et al., 1998)

Df(1)KA9 and Df(1)RK5 embryos exhibit variable defects in the formation of commissural pathways, Scer\GAL4^rho.PL induced expression of P{UAS-NETA} significantly rescued the CNS phenotype. Scer\GAL4^sca-537.4 induced expression leads to a variety of CNS defects; reductions in the number of axons in commissural bundles and more extremely missing commissures.

(Harris et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

NetA^UAS.cHa, Scer\GAL4^how-24B has larval transverse nerve phenotype, suppressible by fra³

(Winberg et al., 1998)

NetA^UAS.cHa, Scer\GAL4^how-24B has larval transverse nerve phenotype, suppressible by fra⁴

(Winberg et al., 1998)

NetA^UAS.cHa, Scer\GAL4^how-24B has larval transverse nerve phenotype, suppressible by Sema2a^UAS.cKa, Scer\GAL4^how-24B

(Winberg et al., 1998)

Suppressor of

Statement

Reference

NetA^UAS.cHa, Scer\GAL4^how-24B is a suppressor of larval transverse nerve phenotype of Scer\GAL4^how-24B, Sema2a^UAS.cKa

(Winberg et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Loss of fra function suppresses the ectopic innervation of muscles 7 and 6 by the transverse nerve caused by Scer\GAL4^how-24B-mediated expression of NetA^Scer\UAS.cHa. Scer\GAL4^how-24B-mediated coexpression of NetA^Scer\UAS.cHa and Sema-2a^Scer\UAS.cKa allows correct innervation by the transverse nerve and the RP3.

(Winberg et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

NetA^UAS.cHa/Scer\GAL4^sim.P3.7 partially rescues Df(1)NetAB^Δ

(Newquist et al., 2013)

NetA^UAS.cHa/Scer\GAL4^AD.elav partially rescues Df(1)NetAB^Δ

(Newquist et al., 2013)

NetA^UAS.cHa/Scer\GAL4^AD.elav partially rescues Df(1)NetAB^Δ

(Newquist et al., 2013)

Fails to rescue

NetA^UAS.cHa/Scer\GAL4^rho.NEE fails to rescue Df(1)NetAB^Δ

(Newquist et al., 2013)

NetA^UAS.cHa/Scer\GAL4^sim.P3.7 fails to rescue Df(1)NetAB^Δ

(Newquist et al., 2013)

NetA^UAS.cHa/Scer\GAL4^rho.NEE fails to rescue Df(1)NetAB^Δ

(Newquist et al., 2013)

NetA^UAS.cHa/Scer\GAL4^sca-109-68 fails to rescue Df(1)NetAB^Δ

(Newquist et al., 2013)

Comments

Expression of NetA^Scer\UAS.cHa under the control of Scer\GAL4^sim.P3.7 almost completely rescues the axon scaffold defects seen in stage 16 Df(1)NetAB^Δ mutant embryos, although a few minor defects are still visible. Expression of NetA^Scer\UAS.cHa under the control of Scer\GAL4^rho.NEE does not rescue the phenotypes, and commissural and longitudinal defects are still present. Expression of NetA^Scer\UAS.cHa in post-mitotic neurons under the control of Scer\GAL4^{AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2} enhances the Df(1)NetAB^Δ axon phenotypes, whereas the increased apoptosis seen in Df(1)NetAB^Δ mutant embryos from stage 14 onwards is partially rescued when NetA^Scer\UAS.cHa is expressed under the control of Scer\GAL4^{AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2}, with embryos displaying an intermediate phenotype that is not statistically significant from either wild type or Df(1)NetAB^Δ.

Expression of NetA^Scer\UAS.cHa under the control of Scer\GAL4^sim.P3.7 rescues the negative geotaxis defects seen in Df(1)NetAB^Δ mutant flies. No rescue is seen when NetA^Scer\UAS.cHa is expressed under the control of either Scer\GAL4^rho.NEE or Scer\GAL4^{AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2}. Expression under the control of either Scer\GAL4^{AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2} and Scer\GAL4^sim.P3.7 (but not Scer\GAL4^rho.NEE) is able to rescue the mechanical-startle induced locomotor defects.

(Newquist et al., 2013)

NetA^Scer\UAS.cHa fails to rescue Df(1)NetAB^Δ when expressed under the control of any of Scer\GAL4^{AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2}, Scer\GAL4^rho.NEE, Scer\GAL4^sca-109-68 or Scer\GAL4^sim.P3.7.

Expression of NetA^Scer\UAS.cHa under the control of any of Scer\GAL4^{AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2}, Scer\GAL4^rho.NEE or Scer\GAL4^sim.P3.7 has no effect on the male courtship 'mirror movement' phenotype seen in Df(1)NetAB^Δ mutants.

Expression of NetA^Scer\UAS.cHa under the control of Scer\GAL4^{AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2} is able to rescue the flightlessness seen in Df(1)NetAB^Δ mutant flies. No rescue is seen when NetA^Scer\UAS.cHa is expressed under the control of either Scer\GAL4^rho.NEE or Scer\GAL4^sca-109-68.

(Newquist et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

NetA^Scer\UAS.cHa

NetA^UAS.cHa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Harris

(Harris et al., 1996)

Secondary FlyBase IDs

References (6)

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