FlyBase Allele Report: Dmel\Mad[1-2]

General Information

Symbol

Dmel\Mad^1-2

Species

D. melanogaster

Name

FlyBase ID

FBal0052185

Feature type

allele

Associated gene

Dmel\Mad

Associated Insertion(s)

P{?lacW}Mad^1-2

Carried in Construct

Also Known As

Mad^1.2, mad¹²

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Firth and Baker, 2005, Kim et al., 1997, Lecuit and Cohen, 1997, Lecuit et al., 1996, Wiersdorff et al., 1996)

Mutagen

P-element activity

(Wiersdorff et al., 1996)

Progenitor genotype

Mad^B1

(Wiersdorff et al., 1996)

Associated Insertion(s)

P{?lacW}Mad^1-2

Cytology

Description

A 5013bp fragment of the P{lacW} transposon is inserted in the first intron of Mad.

(Chen et al., 2022)

Imprecise excision: P{lacW} sequences are retained at the site of the original insertion.

(Wiersdorff et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with Df(2L)C28)

(Sulkowski et al., 2014)

abnormal neuroanatomy | larval stage | somatic clone

(Kaneko et al., 2024)

lethal | recessive

(Wiersdorff et al., 1996)

visible | recessive | somatic clone

(Royet and Finkelstein, 1997)

Phenotype Manifest In

Detailed Description

Statement

Reference

Mad^1-2/Df(2L)C28 mutants show a significant reduction in bouton number at muscles 6/7 and 4 NMJs compared to controls.

(Sulkowski et al., 2014)

R8 photoreceptor differentiation is normal in somatic clones of Mad^1-2 mutant cells.

(Fu and Baker, 2003)

Eye differentiation (as marked by the presence of developing photoreceptors) fails in homozygous clones that touch the posterior margin of the eye disc.

(Curtiss and Mlodzik, 2000)

Homozygous clones induced during the first larval instar stage cause a transformation of ommatidia into frons cuticle.

(Royet and Finkelstein, 1997)

Class II allele. Eye discs are very small. Larval survival rate of homozygotes is 35% of heterozygotes with wild type. Morphogenetic furrow and photoreceptor differentiation are limited to the dorsal region of the disc. Resulting eyes are reduced along the dorsal-ventral axis. Cells do survive in clones. Clones located within the eye field proper show no effect on furrow propagation or eye development in general. Clones at the posterior eye margin cause failure of morphogenetic furrow initiation and pattern duplications. Double mutant clones of Mad^1-2 with Pka-C1^k08918 located in posterior locations show a phenotype similar to Mad single mutant clones, except that they are more frequently associated with overgrowth. Double mutant clones in more anterior positions show a novel phenotype, and are always associated with overgrowth with no ectopic furrow or photoreceptor differentiation. These clones give rise to head cuticle in adult flies.

(Wiersdorff et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Mad^1-2 is an enhancer of partially lethal - majority die phenotype of Neto¹⁰⁹

(Sulkowski et al., 2014)

Suppressor of

Statement

Reference

Mad[+]/Mad^1-2 is a suppressor of visible phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

Other

Statement

Reference

Mad^1-2, myd[+]/myd^3PM71 has abnormal flight phenotype

(Sidisky et al., 2021)

Mad^1-2, Su(H)^del47, ci⁹⁴ has abnormal mitotic cell cycle | somatic clone | cell autonomous phenotype

(Firth and Baker, 2005)

Mad^1-2, Su(H)^del47 has abnormal mitotic cell cycle | somatic clone | cell autonomous phenotype

(Firth and Baker, 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

Mad^1-2 has eye disc | posterior | somatic clone phenotype, suppressible by Scer\GAL4^dpp.blk1/eya^UAS.cBa

(Curtiss and Mlodzik, 2000)

Mad^1-2 has photoreceptor neuron | somatic clone phenotype, suppressible by Scer\GAL4^dpp.blk1/eya^UAS.cBa

(Curtiss and Mlodzik, 2000)

Enhancer of

Statement

Reference

Mad^1-2 is an enhancer of photoreceptor cell R8 & eye disc | somatic clone phenotype of smo³

(Fu and Baker, 2003)

Suppressor of

Statement

Reference

Mad[+]/Mad^1-2 is a suppressor | partially of terminal bouton phenotype of ema^f07675

(Kim et al., 2010)

Mad[+]/Mad^1-2 is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

Other

Statement

Reference

Mad^1-2, Su(H)^del47, ci⁹⁴ has eye disc | somatic clone | cell autonomous phenotype

(Firth and Baker, 2005)

Mad^1-2, Su(H)^del47 has eye disc | somatic clone | cell autonomous phenotype

(Firth and Baker, 2005)

Mad^1-2, Su(H)^del47, ci⁹⁴ has photoreceptor cell R8 & eye disc | somatic clone phenotype

(Fu and Baker, 2003)

Mad^1-2, ci⁹⁴ has photoreceptor cell R8 & eye disc | somatic clone phenotype

(Fu and Baker, 2003)

Mad^1-2, smo³ has eye disc | somatic clone phenotype

(Curtiss and Mlodzik, 2000)

Mad^1-2, smo³ has photoreceptor neuron | somatic clone phenotype

(Curtiss and Mlodzik, 2000)

Additional Comments

Genetic Interactions

Statement

Reference

myd^3PM71/+, Mad^1-2/+ double heterozygotes show a decrease in flight ability.

(Sidisky et al., 2021)

Mad^1-2/+ partially suppresses the synaptic bouton number phenotype of homozygous ema^f07675 mutants.

(Kim et al., 2010)

Eye disc cells simultaneously mutant for Mad^1-2, ci⁹⁴ and Su(H)^del47 arrest in G1 after a severe delay but they never enter the second mitotic wave.

Cells mutant for Mad^1-2 and Su(H)^del47 do not enter the second mitotic wave, while cell cycle entry does occur in cells mutant for Mad^1-2 and ci⁹⁴.

(Firth and Baker, 2005)

R8 photoreceptor differentiation is delayed in ci⁹⁴; Mad^1-2 double mutant clones, and fails in ci⁹⁴; Mad^1-2; Su(H)^del47 triple mutant clones. This is despite normal R8 differentiation in any of the single mutants (although sometimes early in Su(H)^del47 single mutant clones), and in Mad^1-2; Su(H)^del47 double mutants.

(Fu and Baker, 2003)

Eye differentiation is rescued in Mad^1-2 clones by eya^Scer\UAS.cBa expressed under the control of Scer\GAL4^dpp.blk1. smo³ Mad^1-2 double mutant clones in the eye never develop photoreceptors whether they lie at the margin or in the interior of the eye disc. Occasionally an ommatidium forms along the edge of the clone, such that some of the component photoreceptors lie within the clone.

(Curtiss and Mlodzik, 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (5)

Bloomington

7323

w^*; Mad^1-2 P{neoFRT}40A/CyO

53718

w^*; smo³ Mad^1-2 P{neoFRT}40A/CyO

94931

y¹; Df(2L)H15-x4, Mad^1-2 mid^1a5 P{neoFRT}40A/SM1

51287

P{hsFLP}12, y¹ w^*; Df(2L)del47, Mad^1-2 CIAPIN1^del47 Su(H)^del47 P{neoFRT}40A P{l(2)35Bg^+t6.8}2/CyO

51299

P{hsFLP}12, y¹ w^*; Df(2L)del47, Mad^1-2 CIAPIN1^del47 Su(H)^del47 P{neoFRT}40A P{l(2)35Bg^+t6.8}2/CyO; ci⁹⁴/Dp(1;4)1021, y⁺, sv^spa-pol

Notes on Origin

Discoverer

Separable from: Pka-C1^3UTR.

(Chen et al., 2022)

The Mad^1-2 stock contained second-site mutations in Pka-C1; these mutations are represented by the Pka-C1^3UTR allele, and a Mad-mutant chromosome in which the second-site Pka-C1 mutations have been removed is referred to as 'Mad[1-2a]'.

(Chen et al., 2022)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Mad^1-2

(Kaneko et al., 2024, Chen et al., 2022, Ruiz-Losada et al., 2021, Sidisky et al., 2021, Li et al., 2020, La Marca et al., 2019, Li and Baker, 2019, Newcomb et al., 2018, Kim et al., 2017, Ayyaz et al., 2015, Karandikar et al., 2014, Li et al., 2013, Huang et al., 2011, Salzer et al., 2010, Estella et al., 2008, Fromental-Ramain et al., 2008, Oishi et al., 2006, Firth and Baker, 2005)

Mad^1-2a

(Chen et al., 2022)

Mad^1.2

(Svendsen et al., 2019, Svendsen et al., 2015, Joshi et al., 2006, Hays et al., 1999, Minami et al., 1999, Kim et al., 1997, Lecuit and Cohen, 1997, Lecuit et al., 1996)

Mad¹²

(Sulkowski et al., 2014)

mad^1-2

(Kang et al., 2014, Atkins et al., 2013, Chang et al., 2013, Li et al., 2013, Giorgianni and Mann, 2011, Kim et al., 2010, Corrigall et al., 2007, Pappu et al., 2005)

mad¹²

(Ruiz-Losada et al., 2021, Kim et al., 2010)

Name Synonyms

Secondary FlyBase IDs

FBal0052184

References (42)

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