FlyBase Allele Report: Dmel\Mad[B1]

General Information

Symbol

Dmel\Mad^B1

Species

D. melanogaster

Name

FlyBase ID

FBal0052174

Feature type

allele

Associated gene

Dmel\Mad

Associated Insertion(s)

P{lacW}Mad^B1

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Wiersdorff et al., 1996)

Progenitor genotype

Associated Insertion(s)

P{lacW}Mad^B1

Cytology

Description

Insertion of P{lacW} into the first intron of Mad.

(Wiersdorff et al., 1996)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | larval stage

(Wiersdorff et al., 1996)

cell lethal | recessive | somatic clone

(Shen and Dahmann, 2005)

lethal (with Mad^k00223)

(Dunlop et al., 2004)

lethal - all die before end of larval stage | recessive

(Wiersdorff et al., 1996)

some die during larval stage | recessive

(Wiersdorff et al., 1996)

Phenotype Manifest In

Detailed Description

Statement

Reference

Mad^B1/Mad¹² clones in the wing pouch area of the wing disc have very poor survival rates.

(Shen and Dahmann, 2005)

When homozygous clones are made in the eye disc, furrow initiation is blocked in posterior margin cells of the eye disc.

(Janody et al., 2004)

Homozygous germ line clones (either germ line stem cells or spermatocytes) are generated in the testis, but they do not persist to the same extent as wild-type control clones.

(Shivdasani and Ingham, 2003)

Homozygous clones in the posterior of the eye disc do not appear to reorient the morphogenetic furrow in adjacent cells.

(Lee and Treisman, 2001)

20% of the dorsal branch fusion events are disrupted. The fusion cell extends a sprout but does not contact the appropriate fusion partner.

(Steneberg et al., 1999)

Homozygous clones at the posterior margin of the eye disc fail to differentiate as photoreceptors, and often have a non-autonomous inhibitory effect on photoreceptor differentiation in surrounding wild-type cells.

(Hazelett et al., 1998)

Class I allele. Homozygous larvae survive to the late 3rd instar larval stage and lack imaginal discs. Larval fat body and the optic lobes of the CNS are dramatically reduced. Anterior midgut formation is abnormal. Larval survival rate of homozygotes is 25% of heterozygotes with wild type. Clonal analysis revealed that Mad function is autonomously required in the eye imaginal disc cells for proliferation and/or survival.

(Wiersdorff et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Mad^B1 is an enhancer of eye phenotype of sca^hs.roE

(Wiersdorff et al., 1996)

NOT Enhancer of

Statement

Reference

Mad^B1 is a non-enhancer of | heat sensitive phenotype of Chc¹

(Gonzalez-Gaitan and Jackle, 1999)

Suppressor of

Statement

Reference

Mad^B1 is a suppressor of eye phenotype of tkv^Q253D.GMR

(Wiersdorff et al., 1996)

Other

Statement

Reference

Mad^B1, bsk^flp147E has anterior-posterior compartment boundary of the wing disc | somatic clone phenotype

(Shen and Dahmann, 2005)

Mad^B1/Mad¹², brk^XH has anterior-posterior compartment boundary of the wing disc | somatic clone phenotype

(Shen and Dahmann, 2005)

Mad^B1, smo² has eye disc & neuron | somatic clone phenotype

(Brennan et al., 2001)

Mad^B1, smo² has eye disc & nucleus | somatic clone phenotype

(Brennan et al., 2001)

Mad^B1, wg^l-15 has photoreceptor neuron phenotype

(Hazelett et al., 1998)

Mad^B1, wg^l-15 has eye disc | posterior phenotype

(Hazelett et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

brk^XH/brk^XH, Mad^B1/Mad¹² double mutant wing disc clones have normal apical-basal cell length.

(Widmann and Dahmann, 2009)

As Mad^B1 clones in the wing pouch do not survive, and bsk^flp147E single mutant clones show no segregation defects in the wing, Mad^B1 bsk^flp147E clones can be used to study whether Mad^B1 affects segregation of cells at the A/P boundary. The majority of bsk^flp147E Mad^B1 clones of A origin in contact with the A/P boundary of the wing disc are misplaced into the P territory, causing a displacement of the A/P compartment boundary toward P. However, clones of P origin remain in the P compartment. brk^XH Mad^B1/Mad¹² clones generated along the A/P boundary of the wing imaginal disc, using the FLP/FRT technique, show no defects in segregation behaviour. As with wild-type clones, these mutant clones stay in the compartment (anterior or posterior) in which they have been generated.

(Shen and Dahmann, 2005)

Neural differentiation is blocked in smo² ; Mad^B1 double mutant clones in the eye disc. Aberrant nuclear migration is seen in double mutant clones spanning the furrow.

(Brennan et al., 2001)

At 18^oC, 25^oC and 29^oC, Chc¹/+, Mad^B1/+ double heterozygotes have normal wing patterns.

(Gonzalez-Gaitan and Jackle, 1999)

Homozygous Mad^B1 wg^l-15 clones at the posterior margin of the eye disc autonomously fail to initiate photoreceptor development.

(Hazelett et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Mad^k00223

(Dunlop et al., 2004)

Rescued by

Mad^B1 is rescued by Scer\GAL4^Mhc.PW/Mad^UAS.EGFP

(Dudu et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Complements: gkt^A12. Complements: gkt^G85.

(Dunlop et al., 2004)

Excision of the P{lacW} is accompanied by reversion of the Mad mutant phenotype.

(Wiersdorff et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mad^B1

l(2)k5807

mad^B1

(Schwank et al., 2011, Corrigall et al., 2007, Dudu et al., 2006, Shen and Dahmann, 2005, Dunlop et al., 2004, Shivdasani and Ingham, 2003, Bessa et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (20)

Report Sections

Open Close