wing vein L4 & sensillum campaniformium | ectopic | somatic clone
wing vein L4 & wing sensillum | ectopic | somatic clone
Eye disc cells simultaneously mutant for Mad12, ci94 and Su(H)del47 continue proliferating instead of arresting in G1 ahead of the morphogenetic furrow. The same phenotype is seen in cells mutant for Mad12 and ci94 - the presence of Su(H)del47 has no autonomous effect on G1 arrest.
R8 photoreceptor differentiation is delayed in ci94; Mad1-2 and ci94; Su(H)del47 double mutant clones, and fails in ci94; Mad1-2; Su(H)del47 triple mutant clones. This is despite normal R8 differentiation in any of the single mutants (although it is sometimes early in Su(H)del47 single mutant clones), and in Mad1-2; Su(H)del47 double mutants. Ectopic R8 photoreceptor differentiation in ci94; Su(H)del47 somatic clones in eye discs, is much reduced compared to that seen in Su(H)del47 single mutant clones.
When tshScer\UAS.cGa (driven by Scer\GAL469B) is added to ci94 homozygotes, denticles fail to form. When armS10.Scer\UAS.T:Hsap\MYC (driven by Scer\GAL469B) is added to ci94 homozygotes, denticles fail to form, except for the T1 beard, (already present and enlarged in armS10.Scer\UAS.T:Hsap\MYC embryos), which is enhanced by the addition of ci94.
ptc16 ci94 and ptcS2 ci94 double mutant clones in the anterior of the wing disc have "wiggly" boundaries indistinguishable from wild-type control clones. ptc16 ci94 double mutant clones in the wing show the same features as ci94 single mutant clones. hhAC ci94 double mutant embryos are indistinguishable from ci94 single mutant embryos. ci94 wgl-17 double mutant embryos are short and show a "lawn of denticles" phenotype. ci94 ptc16 double mutant embryos show a higher degree of naked cuticle compared with ci94 single mutants. They also differ from ptc16 single mutant embryos. ci94 ptc16 double mutant embryos derived from ci94 ptc16 female germline clones have the same phenotype as ci single mutant embryos.
Df(2R)enE; ci94 double mutant clones of anterior origin in the wing disc straddle the anterior/posterior (A/P) boundary, similar to ci94 single mutant clones. Unlike Df(2R)enE single mutant posterior clones, however, Df(2R)enE; ci94 double mutant clones of posterior origin only partially sort into anterior territory and straddle the A/P boundary (similar to ci94 or Df(2R)enE; ci94 clones originating in the anterior compartment). Df(2R)enE; ci94 double mutant clones of both anterior and posterior origin define straight borders with neighbouring wild-type anterior and posterior cells. Df(2R)enE; ci94 double mutant clones show smooth borders with adjacent wild-type cells when situated entirely within the posterior compartment.
In ptc9 ; ci94 double mutant embryos, naked cuticle is present but is more limited than in ptc9 single mutant embryos. This double mutant cuticle phenotype is "intermediate", resembling neither single mutant. Some segments are fused, and thoracic segments do not produce any naked cuticle. Abdominal denticle diversity is restored. Expansion of denticle types 2 and 3 is seen and type 4 and 5 denticles are seen laterally. The ectopic groove seen in ptc9 single mutant segments is not seen in the double mutant embryos. The mirror image duplication of denticle belts seen in abdominal segments 3, 5 and 7 of embryos expressing hhScer\UAS.cIa under the control of Scer\GAL4prd.RG1 is rescued if they are also mutant for ci94. hhAC ; ci94 double mutant embryos show a cuticle phenotype that is weaker than hhAC but stronger than ci94 single mutant embryos. Type 4 denticles are present. Cuticle from tsh8 ; ci94 double mutant embryos produces only weakly pigmented, type 5 denticles and no naked cuticle.
Hsap\GLIαTub84B.T:Hsap\MYC,T:Ivir\HA1 rescues ci94 mutants to late larval stages. The wing discs of these animals are grossly enlarged. When Hsap\GLI3αTub84B.T:Hsap\MYC,T:Ivir\HA1 is also added, animals die as pupae or late larvae, and exhibit wing discs of normal shape and size.
Expression of ciScer\UAS.cHb under the control of ciScer\UAS.cHb completely rescues the cuticle phenotype of ci94 embryos. Rescued embryos develop into adults that have normal morphology but are sterile.
Expression of ciN:Zn.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ci.PC in ci94 mutants completely restores the wild-type repetitive pattern of denticles and naked cuticle in embryos. These animals complete development and eclose into normal-looking, sterile adults.
Homozygotes are rescued to adulthood by one copy of ci+t16; the rescued flies are fertile, viable and have no obvious phenotypes. ciCe-2/ci94 animals are rescued to adulthood by one copy of ci+t16. These animals show an extreme form of the dominant ciCe-2 phenotype (fusion of veins L3 and L4). ciCe-2/ci94 animals rescued by two copies of ci+t16 do not show fusion of veins L3 and L4. ci94 animals carrying ciU survive to the early pupal stage, but their wing imaginal discs have grossly enlarged anterior compartments.