FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\ine2
Open Close
General Information
Symbol
Dmel\ine2
Species
D. melanogaster
Name
FlyBase ID
FBal0057250
Feature type
allele
Associated gene
Dmel\ine
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Progenitor genotype
    Cytology
    Description

    Mutation is in 'ine-RA' isoform.

    (Huang et al., 2002)

    Amino acid replacement: ?126term.

    (Huang et al., 2002)
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    point_mutation
    2L:4,474,602..4,474,602 [-]
    Nucleotide change:

    C4474602T

    Amino acid change:

    Q126term | ine-PB; Q126term | ine-PC; Q126term | ine-PE; Q126term | ine-PF

    Reported amino acid change:

    ?126term

    Comment:

    Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Curator note: The mutation was reported to be at position 126 with respect to transcript RA, but Figure 3 makes it clear that the position is relative to transcripts RB, RC, RE, and RF, but not RA.

    (Huang et al., 2002)
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        In contrast to control flies, ine2 mutant flies die within 10 days on hypertonic media. As in wild type, no tissue damage is observed in the anterior hindgut.

        The median survival rate of ine2 mutant flies in response to lack of water is reduced compared to wild type controls. Survival rate following starvation (in the presence of sufficient water) is unchanged compared to controls.

        (Luan et al., 2015)

        ine2 flies maintained for 4 days on food medium containing 0M, 0.1M or 0.2M NaCl have similar viability to control wild-type flies. Flies maintained on food medium containing 0.4M NaCl show only slight viability (in contrast to wild type flies, where 90% survive). The flies also show increased sensitivity to KCl and sorbitol compared to wild-type flies.

        (Huang et al., 2002)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Phenotype Manifest In
        Additional Comments
        Genetic Interactions
        Statement
        Reference
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Rescued by

        ine2 is rescued by ineRA.UAS/Scer\GAL4Irk2.PL

        (Luan et al., 2015)

        ine2 is rescued by ineRB.UAS.cLa/Scer\GAL4Irk2.PL

        (Luan et al., 2015)
        Not rescued by

        ine2 is not rescued by Scer\GAL4elav.PU/ineRA.UAS

        (Luan et al., 2015)

        ine2 is not rescued by ineRA.UAS/Scer\GAL4repo.PU

        (Luan et al., 2015)

        ine2 is not rescued by ineRB.UAS.cLa/Scer\GAL4elav.PU

        (Luan et al., 2015)

        ine2 is not rescued by ineRB.UAS.cLa/Scer\GAL4repo.PU

        (Luan et al., 2015)
        Comments

        Expression of ineRA.Scer\UAS in the hindgut under the control of Scer\GAL4Irk2.PL rescues the lethality seen when ine2 mutant flies are raised on hypertonic medium. Expressing ineRA.Scer\UAS in neurons (Scer\GAL4elav.PU) or glia (Scer\GAL4repo.PU) does not rescue the lethality.

        Expression of ineRB.Scer\UAS in the hindgut under the control of Scer\GAL4Irk2.PL rescues the lethality seen when ine2 mutant flies are raised on hypertonic medium. Expressing ineRB.Scer\UAS in neurons (Scer\GAL4elav.PU) or glia (Scer\GAL4repo.PU) does not rescue the lethality.

        Expression of ineRA.Scer\UAS in the hindgut under the control of Scer\GAL4Irk2.PL rescues the increased sensitivity to lack of water seen when ine2 mutant flies. Expressing ineRA.Scer\UAS in neurons (Scer\GAL4elav.PU) or glia (Scer\GAL4repo.PU) does not rescue this increased sensitivity.

        Expression of ineRB.Scer\UAS in the hindgut under the control of Scer\GAL4Irk2.PL rescues the increased sensitivity to lack of water seen when ine2 mutant flies. Expressing ineRB.Scer\UAS in neurons (Scer\GAL4elav.PU) or glia (Scer\GAL4repo.PU) does not rescue this increased sensitivity.

        (Luan et al., 2015)
        Images (0)
        Mutant
        Wild-type
        Stocks (0)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (1)
        Reported As
        Symbol Synonym
        ine2
        (Luan et al., 2015)
        Name Synonyms
        Secondary FlyBase IDs
          References (3)