Expression of ci^{N:Zn.Scer\UAS} under the control of Scer\GAL4^{neur-GAL4-A101} results in the loss of the ventral vdaB class IV neuron in 24% of stage 15 parasegments; however, the more lateral v'ada is never lost. Posterior derived MD neurons are also lost. For example, vbd is lost in 75% of parasegments. Examination of stage 12 embryos reveals that vdaB-loss is preceded by SOP1a loss in 22% of cases. SOP4a are not affected.

Expression of ci^{N:Zn.Scer\UAS} under the control of Scer\GAL4^ems.HRE impairs filzkorper formation but does not affect stigmatophore formation. However, expression of this transgene under the control of Scer\GAL4^salm-459.2 has the reciprocal effect; stigmatophore formation is impaired while filzkorper formation is unaffected.

In ci^{N:Zn.Scer\UAS}; Scer\GAL4^btl.PS embryos, terminal tracheal branches are frequently duplicated. Time-lapse observation shows that the terminal branches initially develop normally, but that secondary terminal branches then form and migrate aberrantly anteriorly. About 1/3 of these duplicated branches arise from a single terminal cells.

Bolwig's organ formation is suppressed in embryos expressing ci^{N:Zn.Scer\UAS} under the control of Scer\GAL4^da.G32. Bolwig's organs are formed in embryos expressing ci^{N:Zn.Scer\UAS} under the control of Scer\GAL4^h-1J3.

Embryos expressing ci^{N:Zn.Scer\UAS} under the control of Scer\GAL4^en-e16E have an abnormal denticle pattern. In the middle of each belt, the denticle rows show a mirror-image 5, 4, 4, 5 pattern (numbers indicate the wild-type denticle row that the altered denticle rows resemble). This pattern is flanked by small denticles pointing away from the belt. The small anterior-pointing denticles at the anterior of each belt could either be type 1 or 6 by morphology.

When expression is driven by Scer\GAL4^ptc-559.1 in the embryo, naked cuticle is deleted and the denticle belts are partially duplicated.