FlyBase Allele Report: Dmel\Ras85D[G13Q.UAS]

General Information

Symbol

Dmel\Ras85D^G13Q.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0058376

Feature type

allele

Associated gene

Dmel\Ras85D

Associated Insertion(s)

Carried in Construct

P{UAS-Ras85B.Act}

Also Known As

UAS-Ras1^Act, RAS1^Act, UAS-Ras1^Q13

Key Links

Transgenic product class

gain_of_function_variant

(Gisselbrecht et al., 1996)

missense_variant

(Gisselbrecht et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

gain_of_function_variant

(Gisselbrecht et al., 1996)

missense_variant

(Gisselbrecht et al., 1996)

Mutagen

in vitro construct

(Gajewski et al., 1998, Michelson et al., 1998, Michelson et al., 1998, Gisselbrecht et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-Ras85B.Act}

Cytology

Description

Expression of an activated form of Ras85D (carries the amino acid replacement G13Q) is governed by UAS regulatory sequences.

(Gisselbrecht et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Ras85D

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA

(Xu et al., 2023)

CEA with Pten^HMS00044, Rbf^HMS03004

(Parkhitko et al., 2021)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of cancer

is exacerbated by ITP^UAS.F

(Xu et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Ras85D^{G13Q.Scer\UAS} under the control of Scer\GAL4^twi.PG results in extra eve-expressing progenitors in the dorsal mesoderm.

(Halfon et al., 2000)

Supernumerary muscle founder cells are formed in embryos expressing Ras85D^{G13Q.Scer\UAS} under the control of Scer\GAL4^twi.PB.

(Schulz and Gajewski, 1999)

Expression of Ras85D^{G13Q.Scer\UAS} under the control of Scer\GAL4^unspecified results in the production of extra eve-positive mesodermal cells in a wild-type background.

(Carmena et al., 1998)

Ras85D^{G13Q.Scer\UAS} expressed under the control of Scer\GAL4^twi.PG in a wild-type background has no effect on early mesodermal migration.

(Michelson et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Ras85D^G13Q.UAS, Scer\GAL4^twi.PG has mesoderm phenotype, enhanceable by twi^UAS.cBa, Scer\GAL4^twi.PG

(Halfon et al., 2000)

Ras85D^G13Q.UAS, Scer\GAL4^twi.PG has mesoderm phenotype, enhanceable by wg^UAS.cGa/twi^UAS.cBa, Scer\GAL4^twi.PG

(Halfon et al., 2000)

Ras85D^G13Q.UAS, Scer\GAL4^twi.PG has mesoderm phenotype, enhanceable by wg^UAS.cGa, Scer\GAL4^twi.PG

(Halfon et al., 2000)

Suppressed by

Statement

Reference

Ras85D^G13Q.UAS, Scer\GAL4^twi.PB has muscle founder cell | increased number phenotype, suppressible by htl^DN.UAS.cMb, Scer\GAL4^twi.PB

(Schulz and Gajewski, 1999)

Ras85D^G13Q.UAS, Scer\GAL4^unspecified has somatic muscle cell | embryonic stage phenotype, suppressible by dpp^H46

(Carmena et al., 1998)

Ras85D^G13Q.UAS, Scer\GAL4^unspecified has somatic muscle cell | embryonic stage phenotype, suppressible by wg^unspecified

(Carmena et al., 1998)

Enhancer of

Statement

Reference

Ras85D^G13Q.UAS, Scer\GAL4^twi.PG is an enhancer of mesoderm phenotype of Scer\GAL4^twi.PG, twi^UAS.cBa

(Halfon et al., 2000)

wg^UAS.cGa/Ras85D^G13Q.UAS, Scer\GAL4^twi.PG is an enhancer of mesoderm phenotype of Scer\GAL4^twi.PG, twi^UAS.cBa

(Halfon et al., 2000)

Suppressor of

Statement

Reference

Scer\GAL4^twi.PB/Ras85D^G13Q.UAS is a suppressor of hindgut visceral muscle fiber phenotype of htl^S1-28

(San Martin and Bate, 2001)

Scer\GAL4^twi.PB/Ras85D^G13Q.UAS is a suppressor of hindgut visceral muscle primordium phenotype of htl^S1-28

(San Martin and Bate, 2001)

Ras85D^G13Q.UAS, Scer\GAL4^twi.PB is a suppressor | partially of muscle founder cell phenotype of Scer\GAL4^twi.PB, htl^DN.UAS.cMb

(Schulz and Gajewski, 1999)

Scer\GAL4^twi.PG/Ras85D^G13Q.UAS is a suppressor of mesoderm phenotype of htl^AB42

(Michelson et al., 1998)

Scer\GAL4^twi.PG/Ras85D^G13Q.UAS is a suppressor of phenotype of stumps^YY202

(Michelson et al., 1998)

Scer\GAL4^twi.PG/Ras85D^G13Q.UAS is a suppressor of mesoderm phenotype of htl^ZZ81

(Gisselbrecht et al., 1996)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of wg^Scer\UAS.cGa and Ras85D^{G13Q.Scer\UAS} under the control of Scer\GAL4^twi.PG results in a lateral expansion of the eve-expressing progenitor clusters in the mesoderm. Co-expression of twi^Scer\UAS.cBa and Ras85D^{G13Q.Scer\UAS} under the control of Scer\GAL4^twi.PG results in a lateral expansion of the eve-expressing progenitor clusters in the mesoderm. Co-expression of twi^Scer\UAS.cBa, Ras85D^{G13Q.Scer\UAS} and wg^Scer\UAS.cGa under the control of Scer\GAL4^twi.PG results in an almost continuous anteroposterior stripe of eve-expressing progenitor cells confined to the dorsal mesoderm.

(Halfon et al., 2000)

An intermediate number of muscle founder cells are produced when both htl^{DN.Scer\UAS.cMb} and Ras85D^{G13Q.Scer\UAS} are co-expressed under the control of Scer\GAL4^twi.PB compared to when either htl^{DN.Scer\UAS.cMb} or Ras85D^{G13Q.Scer\UAS} alone is expressed under the control of Scer\GAL4^twi.PB (no muscle founder cells or supernumerary muscle founder cells respectively).

(Schulz and Gajewski, 1999)

The production of extra eve-positive mesodermal cells caused by expression of Ras85D^{G13Q.Scer\UAS} under the control of Scer\GAL4^unspecified is suppressed if the embryos are also carrying dpp^H46 or wg^unspecified. Expression of Ras85D^{G13Q.Scer\UAS} under the control of Scer\GAL4^unspecified results in the production of extra eve-positive mesodermal cells in mbc^unspecified or stg² background.

(Carmena et al., 1998)

The failure of mesodermal migration in early htl^AB42 embryos is partially rescued by Ras85D^{G13Q.Scer\UAS} expressed under the control of Scer\GAL4^twi.PG.

(Michelson et al., 1998)

Partially rescues the stumps^YY202 phenotype when expressed under the control of Scer\GAL4^twi.PG.

(Michelson et al., 1998)

Partially rescues the htl^ZZ81 phenotype when expressed under the control of Scer\GAL4^twi.PG.

(Gisselbrecht et al., 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

RAS1^Act

(Michelson et al., 1998)

Ras1^Act

(Gisselbrecht et al., 1996)

Ras85D^{G13Q.Scer\UAS}

Ras85D^G13Q.UAS

Name Synonyms

Secondary FlyBase IDs

References (23)

Report Sections

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