FlyBase Allele Report: Dmel\Hsc70-4[D206S.UAS]

General Information

Symbol

Dmel\Hsc70-4^D206S.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0060646

Feature type

allele

Associated gene

Dmel\Hsc70-4

Associated Insertion(s)

Carried in Construct

P{UAS-Hsc70-4.D206S}

Key Links

Transgenic product class

missense_variant

(Elefant and Palter, 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Elefant and Palter, 1999)

Mutagen

in vitro construct

(Elefant and Palter, 1999, Elefant and Palter, 1997)

Progenitor genotype

Hsc70-4^UAS.cEa

(Elefant and Palter, 1999)

Carried in construct

P{UAS-Hsc70-4.D206S}

Cytology

Description

Amino acid replacement: D206S.

(Elefant and Palter, 1999)

Encodes a Hsc70-4 protein with a single point mutation in the ATP binding site.

(Elefant and Palter, 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsc70-4

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior, with Scer\GAL4^156Y

(Elefant and Palter, 1999)

lethal, with Scer\GAL4^how-24B

(Elefant and Palter, 1999)

lethal | dominant, with Scer\GAL4^unspecified

(Elefant and Palter, 1997)

lethal - all die before end of larval stage, with Scer\GAL4^337Y

(Elefant and Palter, 1999)

some die during larval stage, with Scer\GAL4^337Y

(Elefant and Palter, 1999)

Phenotype Manifest In

larval muscle system, with Scer\GAL4^how-24B

(Elefant and Palter, 1999)

Detailed Description

Statement

Reference

Animals expressing Hsc70-4^{D206S.Scer\UAS} under the control of Scer\GAL4^337Y die as first or second instar larvae. Animals expressing Hsc70-4^{D206S.Scer\UAS} under the control of Scer\GAL4^how-24B generally die as embryos or first instar larvae. Animals expressing Hsc70-4^{D206S.Scer\UAS} under the control of Scer\GAL4^how-24B that die as embryos are unable to puncture the egg membrane and hatch and those larvae that do hatch are unable to crawl. Embryos expressing Hsc70-4^{D206S.Scer\UAS} under the control of Scer\GAL4^how-24B have a range of severe muscle pattern defects, including gaps in the muscle pattern indicating loss of muscle fibres and irregularities in the muscle pattern. The majority of newly hatched or 2 day old larvae expressing Hsc70-4^{D206S.Scer\UAS} under the control of Scer\GAL4^156Y are unable to locate a yeast paste food source within 20 minutes in a behavioural assay.