FlyBase Allele Report: Dmel\arm[S2.UAS.Tag:MYC]

General Information

Symbol

Dmel\arm^{S2.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0061500

Feature type

allele

Associated gene

Dmel\arm

Associated Insertion(s)

Carried in Construct

P{UAS-arm.S2}

Also Known As

UAS-arm^S2, UAS-Arm, Arm-WT

Key Links

Transgenic product class

wild_type

(Pai et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Pai et al., 1997)

Mutagen

in vitro construct

(Pai et al., 1997)

Progenitor genotype

Carried in construct

P{UAS-arm.S2}

Cytology

Description

A wild-type arm protein, tagged with Tag:MYC, is expressed under the control of UASt regulatory sequences.

(Pai et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

arm

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neuropathy

CEA

(Hale et al., 2022)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^Mef2.PR

(Lee et al., 2014)

viable, with Scer\GAL4^e22c

(Pai et al., 1997)

Phenotype Manifest In

adult abdominal fat mass, with Scer\GAL4^Mef2.PR

(Lee et al., 2014)

lipid droplet | adult stage, with Scer\GAL4^Mef2.PR

(Lee et al., 2014)

wing, with Scer\GAL4^30A

(Kiger et al., 2007)

Detailed Description

Statement

Reference

Adults expressing arm^{S2.UAS.Tag:MYC} under the control of Scer\GAL4^Mef2.PR have a reduced abdominal fat body with smaller lipid droplets than controls.

(Lee et al., 2014)

Expression of arm^{S2.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^{Mef2.247.Switch}, has no effect on long-term memory (regardless of the presence or absence of RU486 in the food).

(Tan et al., 2013)

Expression of arm^{S2.Scer\UAS.T:Hsap\MYC} in the heart under the control of Scer\GAL4^tin.CΔ4 does not appear to alter cardiac function as fractional shortening is comparable with controls.

(Casad et al., 2012)

Expression of arm^{S2.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act88F.PD does not result in a mutant wing phenotype.

(Morris et al., 2008)

Expression of arm^{S2.Scer\UAS.T:Hsap\MYC} in the wing under the control of Scer\GAL4^30A has no effect; all cells delaminate, leave the wing, and wing surfaces bond normally.

(Kiger et al., 2007)

Somatic clones overexpressing arm^{S2.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^e22c, display 'wiggly' clone borders, with normal cell adhesion properties.

(Jaiswal et al., 2006)

Expression of arm^{S2.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^e22c has no effect on the embryonic pattern.

(Cox et al., 1999)

arm^{S2.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^C96 flies appear normal.

(Helms et al., 1999)

Expression of arm^{S2.Scer\UAS.T:Hsap\MYC} using Scer\GAL4^e22c or Scer\GAL4^69B has no phenotypic consequences in the embryo, and animals expressing arm^{S2.Scer\UAS.T:Hsap\MYC} using Scer\GAL4^e22c survive to adulthood. The integrity of the cuticle of embryos that are zygotically and maternally mutant for arm³ is dramatically improved if these embryos are also expressing arm^{S2.Scer\UAS.T:Hsap\MYC} (using Scer\GAL4^e22c), some of the secreted cuticle has denticles.

(Pai et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

arm^{S2.UAS.Tag:MYC}, Scer\GAL4^hs.2sev is an enhancer of abnormal cell polarity phenotype of CadN^UAS.cIa, Scer\GAL4^hs.2sev

(Mirkovic and Mlodzik, 2006)

Suppressor of

Statement

Reference

arm^{S2.UAS.Tag:MYC}, Scer\GAL4^Act88F.PD is a suppressor | partially of visible phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Morris et al., 2008)

Scer\GAL4^vg.PM/arm^{S2.UAS.Tag:MYC} is a suppressor of visible phenotype of Scer\GAL4^vg.PM, shg^UAS.cOa

(Kataoka et al., 2001)

NOT Suppressor of

Statement

Reference

arm^{S2.UAS.Tag:MYC}, Scer\GAL4^bbg-C96 is a non-suppressor of visible phenotype of Klp64D^NIG.10642R, Scer\GAL4^bbg-C96

(Vuong et al., 2014)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^hs.2sev/arm^{S2.UAS.Tag:MYC} is an enhancer of ommatidium phenotype of CadN^UAS.cIa

(Mirkovic and Mlodzik, 2006)

Suppressor of

Statement

Reference

arm^{S2.UAS.Tag:MYC}, Scer\GAL4^Act88F.PD is a suppressor of wing phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Morris et al., 2008)

Scer\GAL4^vg.PM/arm^{S2.UAS.Tag:MYC} is a suppressor of wing margin phenotype of Scer\GAL4^vg.PM, shg^UAS.cOa

(Kataoka et al., 2001)

NOT Suppressor of

Statement

Reference

arm^{S2.UAS.Tag:MYC}, Scer\GAL4^bbg-C96 is a non-suppressor of wing margin phenotype of Klp64D^NIG.10642R, Scer\GAL4^bbg-C96

(Vuong et al., 2014)

Scer\GAL4^vg.PM/arm^{S2.UAS.Tag:MYC} is a non-suppressor of wing margin phenotype of tws^j11C8

(Bajpai et al., 2004)

Scer\GAL4^bbg-C96/arm^{S2.UAS.Tag:MYC} is a non-suppressor of wing phenotype of Scer\GAL4^bbg-C96, mam^N.UAS

(Helms et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of arm^{S2.Scer\UAS.T:Hsap\MYC} does not suppress the wing notching seen when Klp64D^NIG.10642R is expressed under the control of Scer\GAL4^bbg-C96.

(Vuong et al., 2014)

Co-expression of arm^{S2.Scer\UAS.T:Hsap\MYC} strongly suppresses the gnarled wing phenotype caused by expression of E2f^Scer\UAS.cNa under the control of Scer\GAL4^Act88F.PD.

(Morris et al., 2008)

Coexpression of arm^{S2.Scer\UAS.T:Hsap\MYC} enhances the ommatidial rotational defects of Scer\GAL4^hs.2sev>CadN^Scer\UAS.cIa mutants and causes the loss of some photoreceptors.

(Mirkovic and Mlodzik, 2006)

Does not suppress the wing nicking phenotype seen in mam^N.Scer\UAS, Scer\GAL4^C96 flies.

(Helms et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^{Mef2.247.Switch}/arm^{S2.UAS.Tag:MYC} rescues arm^GD1372

(Tan et al., 2013)

Scer\GAL4^e22c/arm^{S2.UAS.Tag:MYC} rescues arm⁴

(Pai et al., 1997)

Partially rescues

Scer\GAL4^e22c/arm^{S2.UAS.Tag:MYC} partially rescues arm²

(Cox et al., 1999)

Fails to rescue

Scer\GAL4^e22c/arm^{S2.UAS.Tag:MYC} fails to rescue arm³

(Cox et al., 1999)

Comments

Co-expression of arm^{S2.Scer\UAS.T:Hsap\MYC} rescues the long term memory of RU486-fed arm^GD1372/+; Scer\GAL4^{Mef2.247.Switch}/+ flies.

(Tan et al., 2013)

Either completely or substantially rescues the segment polarity phenotype of embryos that are maternally and zygotically mutant for arm², when expressed under the control of Scer\GAL4^e22c.

(Cox et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

4783

y¹ w¹¹¹⁸; P{UAS-arm.S2}A1

Kyoto

107878

y¹ w¹¹¹⁸; P{UAS-arm.S2}A1

Notes on Origin