FlyBase Allele Report: Dmel\E(spl)m8-HLH[1.UAS]

General Information

Symbol

Dmel\E(spl)m8-HLH^1.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0063458

Feature type

allele

Associated gene

Dmel\E(spl)m8-HLH

Associated Insertion(s)

Carried in Construct

P{UAS-E(spl)m8-HLH^D}

Also Known As

UAS-m8*, UAS-m8^*

Key Links

Transgenic product class

dominant_negative_variant

(Giebel and Campos-Ortega, 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Giebel and Campos-Ortega, 1997)

Mutagen

in vitro construct

(Giebel and Campos-Ortega, 1997)

Progenitor genotype

Carried in construct

P{UAS-E(spl)m8-HLH^D}

Cytology

Description

UASt regulatory sequences drive expression of a cDNA fragment which encodes the protein produced in E(spl)m8-HLH¹ mutants (the protein lacks the C-terminal 56 amino acid residues of wild-type E(spl)m8-HLH and has nine new amino acids in their place). The encoded protein acts as a dominant negative (as assayed by effects on bristle development).

(Giebel and Campos-Ortega, 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

E(spl)m8-HLH

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^unspecified

(Nagel et al., 1999)

Phenotype Manifest In

adult abdomen & macrochaeta, with Scer\GAL4^sca-537.4

(Giebel and Campos-Ortega, 1997)

adult head & macrochaeta, with Scer\GAL4^sca-537.4

(Giebel and Campos-Ortega, 1997)

eye, with Scer\GAL4^unspecified

(Nagel et al., 1999)

macrochaeta | ectopic & scutellum, with Scer\GAL4^455.2

(Karandikar et al., 2004)

scutellar bristle | ectopic, with Scer\GAL4^455.2

(Kahali et al., 2009)

scutellar bristle | ectopic, with Scer\GAL4^sca-537.4

(Kahali et al., 2009)

scutum & macrochaeta & adult, with Scer\GAL4^sca-537.4

(Giebel and Campos-Ortega, 1997)

Detailed Description

Statement

Reference

Expression of E(spl)m8-HLH^1.Scer\UAS with Scer\GAL4^sca-537.4 has no effect on the eye or interommatidial bristles, but results in significantly more flies with ectopic scutellar macrochaetes than controls.

Expression of E(spl)m8-HLH^1.Scer\UAS with Scer\GAL4^455.2 results in ectopic scutellar macrochaetes.

(Kahali et al., 2009)

Expression of E(spl)^1.Scer\UAS under the control of Scer\GAL4^h-H10 leads to eyes of wild-type appearance.

(Bose et al., 2006)

When E(spl)^Scer\UAS.cKa is driven by Scer\GAL4^455.2, the number of macrochaetae seen on the scutellum is increased from 4 to about 5.5. When E(spl)^1.Scer\UAS is driven by Scer\GAL4^sca-537.4 no reduction in the eye is seen. When E(spl)^1.Scer\UAS is driven by Scer\GAL4^h-1J3, Scer\GAL4^GMR.PF or Scer\GAL4^hs.2sev no eye phenotype is seen.

(Karandikar et al., 2004)

When E(spl)^1.Scer\UAS is expressed in the eye under the control of Scer\GAL4^unspecified, a weak eye phenotype is seen.

(Nagel et al., 1999)

Scer\GAL4^sca-537.4-mediated expression enhances development of bristles on the notum and causes development of split bristles on the abdomen and head.

(Giebel and Campos-Ortega, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

E(spl)m8-HLH^1.UAS/Scer\GAL4^h-1J3 is an enhancer of eye phenotype of N^spl-1

(Nagel et al., 1999)

E(spl)m8-HLH^1.UAS, Scer\GAL4^455.2 is an enhancer of scutellar bristle | ectopic phenotype of Scer\GAL4^455.2, l(1)sc^UAS.cHa

(Giebel and Campos-Ortega, 1997)

NOT Enhancer of

Statement

Reference

E(spl)m8-HLH^1.UAS/Scer\GAL4^sca-109-68 is a non-enhancer of eye phenotype of N^spl-1

(Kahali et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of E(spl)m8-HLH^1.Scer\UAS mediated by Scer\GAL4^h-H10 in a N^spl-1/Y background elicits a severely reduced eye.

(Kahali et al., 2009)

Expression of E(spl)^1.Scer\UAS under the control of Scer\GAL4^h-H10 in a N^spl-1/Y background results in reduced eyes that are similar in appearance to those seen in N^spl-1/Y single mutants.

(Bose et al., 2006)

Strong increase in the number of ectopic scutellar macrochaetes of strain ZB1.4 (activator insertion Scer\GAL4^455.2 is combined with l(1)sc^Scer\UAS.cHa).

(Giebel and Campos-Ortega, 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

E(spl)^1.Scer\UAS

E(spl)^1.UAS

E(spl)m8-HLH^1.Scer\UAS

E(spl)m8-HLH^1.UAS

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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