FlyBase Allele Report: Dmel\Bsg[k13638]

General Information

Symbol

Dmel\Bsg^k13638

Species

D. melanogaster

Name

FlyBase ID

FBal0064127

Feature type

allele

Associated gene

Dmel\Bsg

Associated Insertion(s)

P{lacW}Bsg^k13638

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Curtin et al., 2005)

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}Bsg^k13638

Cytology

Description

P-element insertion in Bsg, 1145bp upstream of the ATG codon of the Bsg-265 isoform and 981bp upstream of the start of the first coding exon.

(Curtin et al., 2005)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:8,088,375..8,088,375 [+]

(Besse et al., 2007, Curtin et al., 2005, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates autosomal recessive intellectual developmental disorder

modeled by Nab2^EP3716

(Rounds et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

lethal - all die before end of larval stage | recessive

(Curtin et al., 2005)

partially lethal - majority die | larval stage (with Df(2L)Exel7034)

(Besse et al., 2007)

partially lethal - majority die | late embryonic stage (with Df(2L)Exel7034)

(Besse et al., 2007)

some die during larval stage | recessive

(Curtin et al., 2005)

Phenotype Manifest In

neuron & eye photoreceptor cell | somatic clone

(Curtin et al., 2005)

Detailed Description

Statement

Reference

Bsg^k13638/Df(2L)Exel7034 transheterozygotes are embryonic/early larval lethal.

(Besse et al., 2007)

Mosaic animals in which the eyes are homozygous for Bsg^k13638 mostly show normal location of photoreceptor nuclei in the retina, although a few of the nuclei are displaced.

(Curtin et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Bsg^k13638/Bsg[+] is a suppressor | partially of visible | adult stage phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Rounds et al., 2022)

Bsg^k13638/Bsg[+] is a suppressor of abnormal eye color phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Rounds et al., 2022)

Phenotype Manifest In

Enhanced by

Statement

Reference

Bsg^k13638 has neuron & eye photoreceptor cell | somatic clone phenotype, enhanceable by mys^b45

(Curtin et al., 2005)

Suppressor of

Statement

Reference

Bsg^k13638/Bsg[+] is a suppressor | partially of eye phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Rounds et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

The phenotype of mild displacement of photoreceptor nuclei that is seen in the retina of mosaic animals in which the eyes are homozygous for Bsg^k13638 is enhanced if the animals are also hemizygous for mys^b45.

(Curtin et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

Bsg^k06338

(BDGP Project Members, 1994-1999)

Bsg^k07602

(BDGP Project Members, 1994-1999)

Bsg^k09030

(BDGP Project Members, 1994-1999)

Bsg^k13510

(BDGP Project Members, 1994-1999)

Fails to complement

Bsg⁰⁶²⁴³

(Curtin et al., 2005)

Bsg^k04604

(BDGP Project Members, 1994-1999)

Bsg^k09402

(BDGP Project Members, 1994-1999)

Rescued by

Df(2L)Exel7034/Bsg^k13638 is rescued by Scer\GAL4^Tub.PU/Bsg^UAS.cBa

(Besse et al., 2007)

Df(2L)Exel7034/Bsg^k13638 is rescued by Scer\GAL4^elav-C155/Bsg^{Δintra.UAS.GFP}

(Besse et al., 2007)

Partially rescued by

Df(2L)Exel7034/Bsg^k13638 is partially rescued by Scer\GAL4^elav-C155/Bsg^NGG.UAS.GFP

(Besse et al., 2007)

Not rescued by

Df(2L)Exel7034/Bsg^k13638 is not rescued by Scer\GAL4^elav-C155/Bsg^{extra.UAS.Tag:M(rCd2),GFP}

(Besse et al., 2007)

Df(2L)Exel7034/Bsg^k13638 is not rescued by Bsg^NGG.UAS.GFP/Scer\GAL4^Tub.PU

(Besse et al., 2007)

Comments

Bsg^k13638/Df(2L)Exel7034 transheterozygote lethality is rescued through expression of Bsg^Scer\UAS.cBa under the control of Scer\GAL4^tub.

Expression of Bsg^{Δextra.T:Rnor\CD2.Scer\UAS.T:Avic\GFP} presynaptically (using Scer\GAL4^elav-C155) fails to rescue defects in bouton size and number and only poorly rescues bouton growth defects in Bsg^k13638/Df(2L)Exel7034 transheterozygotes.

Expression of Bsg^{Δextra.Scer\UAS.T:Avic\GFP} presynaptically (using Scer\GAL4^elav-C155) fails to rescue defects in bouton size and number and only poorly rescues bouton growth defects in Bsg^k13638/Df(2L)Exel7034 transheterozygotes.

Expression of Bsg^{NGG.Scer\UAS.T:Avic\GFP} presynaptically (using Scer\GAL4^elav-C155) poorly rescues defects in bouton size and number in Bsg^k13638/Df(2L)Exel7034 transheterozygotes.

Ubiquitous expression of Bsg^{NGG.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^tub does not significantly rescue the early lethality associated with Bsg^k13638/Df(2L)Exel7034.

Expression of Bsg^{Δintra.Scer\UAS.T:Avic\GFP} presynaptically (using Scer\GAL4^elav-C155) rescues defects in bouton size and number in Bsg^k13638/Df(2L)Exel7034 transheterozygotes.

(Besse et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

11096

y¹ w^67c23; P{lacW}Bsg^k13638/CyO

Kyoto

111327

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}Bsg^k13638 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Complements: Cka⁰⁵⁸³⁶. Complements: l(2)k06327^k06327. Complements: l(2)k16919^k16919. Complements: l(2)s4199^s4199.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Bsg^k13638

(Rounds et al., 2022)

P1096

(Curtin et al., 2005)

gel^k13638

l(2)k13638

(Spradling et al., 1999)

l(2)k13638^k13638

(BDGP Project Members, 1994-1999)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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